Crystallization, data collection and data processing of maltose-binding protein (MalE) from the phytopathogen Xanthomonas axonopodis pv. citri

Maltose-binding protein is the periplasmic component of the ABC transporter responsible for the uptake of maltose/maltodextrins. The Xanthomonas axonopodis pv. citri maltose-binding protein MalE has been crystallized at 293 Kusing the hanging-drop vapour-diffusion method. The crystal belonged to the primitive hexagonal space group P6_122, with unit-cell parameters a = 123.59, b = 123.59, c = 304.20 Å, and contained two molecules in the asymetric unit. It diffracted to 2.24 Å resolution

Mass Generation from Lie Algebra Extensions

Applied to the electroweak interactions, the theory of Lie algebra extensions suggests a mechanism by which the boson masses are generated without resource to spontaneous symmetry breaking. It starts from a gauge theory without any additional scalar field. All the couplings predicted by the Weinberg-Salam theory are present, and a few others which are nevertheless consistent within the model.Comment: 11 pages; revtex; title and PACS have been changed; comments included in the manuscrip

Analytical results for a Bessel function times Legendre polynomials class integrals

When treating problems of vector diffraction in electromagnetic theory, the evaluation of the integral involving Bessel and associated Legendre functions is necessary. Here we present the analytical result for this integral that will make unnecessary numerical quadrature techniques or localized approximations. The solution is presented using the properties of the Bessel and associated Legendre functions.Comment: 4 page

The Stellar Content of Obscured Galactic Giant HII Regions. VI: W51A

We present K-band spectra of newly born OB stars in the obscured Galactic giant H II region W51A and ~ 0.8'' angular resolution images in the J, H and K_S-bands. Four objects have been spectroscopically classified as O-type stars. The mean spectroscopic parallax of the four stars gives a distance of 2.0 \pm 0.3 kpc (error in the mean), significantly smaller than the radio recombination line kinematic value of 5.5 kpc or the values derived from maser propermotion observations (6--8 kpc). The number of Lyman continuum photons from the contribution of all massive stars (NLyc ~ 1.5 x 10^{50} s^{-1}) is in good agreement with that inferred from radio recombination lines (NLyc = 1.3 x 10^{50} s^{-1}) after accounting for the smaller distance derived here. We present analysis of archival high angular resolution images (NAOS CONICA at VLT and T-ReCS at Gemini) of the compact region W51 IRS2. The K_S--band images resolve the infrared source IRS~2 indicating that it is a very young compact HII region. Sources IRS2E was resolved into compact cluster (within 660 AU of projected distance) of 3 objects, but one of them is just bright extended emission. W51d1 and W51d2 were identified with compact clusters of 3 objects (maybe 4 in the case of W51d1) each one. Although IRS~2E is the brightest source in the K-band and at 12.6 \micron, it is not clearly associated with a radio continuum source. Our spectrum of IRS~2E shows, similar to previous work, strong emission in Br$\gamma$ and HeI, as well as three forbidden emission lines of FeIII and emission lines of molecular hydrogen (H_2) marking it as a massive young stellar object.Comment: 31 pages and 9 figures, submitted to A

Solution of an associating lattice gas model with density anomaly on a Husimi lattice

We study a model of a lattice gas with orientational degrees of freedom which resemble the formation of hydrogen bonds between the molecules. In this model, which is the simplified version of the Henriques-Barbosa model, no distinction is made between donors and acceptors in the bonding arms. We solve the model in the grand-canonical ensemble on a Husimi lattice built with hexagonal plaquettes with a central site. The ground-state of the model, which was originally defined on the triangular lattice, is exactly reproduced by the solution on this Husimi lattice. In the phase diagram, one gas and two liquid (high density-HDL and low density-LDL) phases are present. All phase transitions (GAS-LDL, GAS-HDL, and LDL-HDL) are discontinuous, and the three phases coexist at a triple point. A line of temperatures of maximum density (TMD) in the isobars is found in the metastable GAS phase, as well as another line of temperatures of minimum density (TmD) appears in the LDL phase, part of it in the stable region and another in the metastable region of this phase. These findings are at variance with simulational results for the same model on the triangular lattice, which suggested a phase diagram with two critical points. However, our results show very good quantitative agreement with the simulations, both for the coexistence loci and the densities of particles and of hydrogen bonds. We discuss the comparison of the simulations with our results.Comment: 12 pages, 5 figure

Post-activation Potentiation In Propulsive Force After Specific Swimming Strength Training

We investigated whether a conditioning activity (8x12.5m with 2.5min-interval using both hand paddles and parachute) induced post-activation potentiation in swimming propulsive force and whether a swimmer's force level affected a post-activation potentiation response. 8 competitive swimmers (5 males and 3 females, age: 18.4 +/- 1.3 years; IPS=796 +/- 56) performed a 10s maximum tethered swimming test 8 and 4min before (the highest value was considered as PRE), and 2.5 and 6.5min after (POST1 and POST2, respectively) the conditioning activity. Rate of force development was not affected, but peak force in POST1 (p=0.02) and impulse in both POST1 (p=0.007) and POST2 (p=0.004) were reduced. Possibly the conditioning activity induced greater fatigue than post-activation potentiation benefits. For instance, the number of repetitions might have been excessive, and rest intervals between the conditioning activity and POST1 and POST2 were possibly too short. There were positive correlations between PRE peak force and changes in peak force and rate of force development. Although conditioning activity was detrimental, positive correlations suggest that weaker swimmers experience a deterioration of performance more than the stronger ones. This conditioning activity is not recommended for swimmers with the current competitive level before a competitive event.37431331

Network conduciveness with application to the graph-coloring and independent-set optimization transitions

We introduce the notion of a network's conduciveness, a probabilistically interpretable measure of how the network's structure allows it to be conducive to roaming agents, in certain conditions, from one portion of the network to another. We exemplify its use through an application to the two problems in combinatorial optimization that, given an undirected graph, ask that its so-called chromatic and independence numbers be found. Though NP-hard, when solved on sequences of expanding random graphs there appear marked transitions at which optimal solutions can be obtained substantially more easily than right before them. We demonstrate that these phenomena can be understood by resorting to the network that represents the solution space of the problems for each graph and examining its conduciveness between the non-optimal solutions and the optimal ones. At the said transitions, this network becomes strikingly more conducive in the direction of the optimal solutions than it was just before them, while at the same time becoming less conducive in the opposite direction. We believe that, besides becoming useful also in other areas in which network theory has a role to play, network conduciveness may become instrumental in helping clarify further issues related to NP-hardness that remain poorly understood

Early appraisal of the fixation probability in directed networks

In evolutionary dynamics, the probability that a mutation spreads through the whole population, having arisen in a single individual, is known as the fixation probability. In general, it is not possible to find the fixation probability analytically given the mutant's fitness and the topological constraints that govern the spread of the mutation, so one resorts to simulations instead. Depending on the topology in use, a great number of evolutionary steps may be needed in each of the simulation events, particularly in those that end with the population containing mutants only. We introduce two techniques to accelerate the determination of the fixation probability. The first one skips all evolutionary steps in which the number of mutants does not change and thereby reduces the number of steps per simulation event considerably. This technique is computationally advantageous for some of the so-called layered networks. The second technique, which is not restricted to layered networks, consists of aborting any simulation event in which the number of mutants has grown beyond a certain threshold value, and counting that event as having led to a total spread of the mutation. For large populations, and regardless of the network's topology, we demonstrate, both analytically and by means of simulations, that using a threshold of about 100 mutants leads to an estimate of the fixation probability that deviates in no significant way from that obtained from the full-fledged simulations. We have observed speedups of two orders of magnitude for layered networks with 10000 nodes