Vrednotenje strupenosti in genotoksičnosti vzorcev zemlje z biotesti

Soil samples were obtained from Slovenian industrial region Šaleska dolina and aqueous leachates were prepared. The toxicity and genotoxicity potential of these aqueous fractions have been evaluated by in vitro toxicity and genotoxicity bioassays. Freshwater toxicity test (PROTOXKIT FTM) with a ciliate protozoan Tetrahymena thermophila, and genotoxicity comet assay using Tetrahymena thermophila, Caco-2 and HepG2 cell lines were performed. Biological data were completed through chemical analyses. It has been shown that physico-chemical analyses alone may not be sufficient to characterize soil hazards. To study soil ecotoxcity it is therefore necessary to take into consideration both, the physico-chemical analyses, toxicity and genotoxicity assays.Na industrijskem področju Slovenije, v Šaleški dolini, smo vzorčili zemljo in pripravili vodne izlužke zemlje iz štirih vzrčnih mest. Toksični in genotoksični potencial vodnih izlužkov smo ovrednotili z uporabo in vitro biotestov za toksičnost in genotoksičnost. Izvedli smo test toksičnosti s sladkovodno praživaljo, migetalkarjem, Tetrahymena thermophila, s t.i. PROTOXKIT FTM testom in test genotoksičnosti: kometni test s praživaljo Tetrahymena thermophila, in Caco-2 ter HepG2 celičnima linijama. Rezultate biotestov smo dopolnili s fizikalno-kemijskimi analizami. Za relevantnejše ekotoksikološko vrednotenje vzorcev zemlje je pomembno združiti fizikalno-kemijske analize in teste toksičnosti in genotoksičnosti