Faculty versus Student Repeatability on Evaluating Translucency of the Anterior Dentition

The objective was to compare the repeatability between dental faculty, whose clinical practice was primarily restorative dentistry, and final year dental students in categorizing the inherent translucency of images selected at random using either a 3- or 7-point scale (translucent to opaque). Digital images of anterior dentition were randomly selected based on inherent translucency. Thirty images (five were repeated) were randomized and categorized by 20 dental students and 20 faculty on their inherent translucency. Statistical analysis was performed using an F test for analysis of variance at 95% confidence interval. A covariance parameter estimate (CPE) was accomplished to compare the inter-rater variability of the dental faculty and dental students. Statistically, more variability occurred between Slides (CPE of 0.185 (p = 0.001)) and between Subject and Slide (CPE of 0.122 (p = 0.0002)) than within subjects (CPE of 0.021 (p = 0.083)). Viewing repeat Slides, Students (CPE = 0.16) were more consistent (p \u3c 0.05) than faculty (CPE = 1.8) using the 3- point scale, while the CPE was the same (CPE = 0.669) using 7-point scale. Dental students and faculty were consistent using the 7-point scale to judge repeat slides, while dental students in this limited pilot study were more consistent when viewing a repeat slide using the 3-point scale

Polymerization cycles on hardness and surface gloss of denture bases

The aim of this study was to evaluate different polymerization cycles on the hardness and surface gloss of acrylic resins for denture bases. Classico and Vip Cril Plus acrylic resins samples were made in dental stone molds. Powder-liquid ratio and resin manipulation were according to the manufacturers’ instructions, and the resulting mass pressed in metallic fl asks. The polymerization cycles were A - hot water bath at 74°C for 9 hours; B - hot water bath at 74°C for 8 hours + 100°C for 1 hour, and C - hot water bath at 74°C for 2 hours + 100°C for 1 hour. After polymerization, the samples were defl asked and submitted to finishing and polishing procedures, and stored in water at 37°C for 24 hours. A hardness indenter with load of 25 gf for 10 s evaluated the Knoop hardness values. A gloss meter evaluated the surface gloss using a light incidence of 60°. Data were submitted to ANOVA and Tukey’s test (α = 0.05). Hardness: There were signifi cant differences between resins regardless of polymerization cycles (Classico = 22.28 and Vip Cril Plus = 25.83). Significant differences occurred among polymerization cycles regardless of resins (A = 25.83, B = 24.64 and C = 21.73). There was similarity for the resin and cycle interaction (Classico: A = 24.51, B = 22.68 and C = 19.65; Vip Cril Plus: A = 27.15, B = 26.53 and C = 23.81). Surface gloss. Significant differences were shown between resins regardless of polymerization cycle (Classico = 57.26 and Vip Cril Plus = 49.38) and between polymerization cycles regardless of resin (A = 48.82, B = 53.46 and C = 57.68). Statistical diff erences were also found for the resin and cycle interaction (Classico: A = 52.32, B = 63.79 and C = 55.67; Vip Cril Plus: A = 45.32, B = 43.14 and C = 59.69). Diff erent polymerization cycles showed similar eff ects on hardness and different effects on the surface gloss of denture base acrylic resins

Faculty versus Student Repeatability on Evaluating Translucency of the Anterior Dentition

The objective was to compare the repeatability between dental faculty, whose clinical practice was primarily restorative dentistry, and final year dental students in categorizing the inherent translucency of images selected at random using either a 3- or 7-point scale (translucent to opaque). Digital images of anterior dentition were randomly selected based on inherent translucency. Thirty images (five were repeated) were randomized and categorized by 20 dental students and 20 faculty on their inherent translucency. Statistical analysis was performed using an F test for analysis of variance at 95% confidence interval. A covariance parameter estimate (CPE) was accomplished to compare the inter-rater variability of the dental faculty and dental students. Statistically, more variability occurred between Slides (CPE of 0.185 (p = 0.001)) and between Subject and Slide (CPE of 0.122 (p = 0.0002)) than within subjects (CPE of 0.021 (p = 0.083)). Viewing repeat Slides, Students (CPE = 0.16) were more consistent (p < 0.05) than faculty (CPE = 1.8) using the 3- point scale, while the CPE was the same (CPE = 0.669) using 7-point scale. Dental students and faculty were consistent using the 7-point scale to judge repeat slides, while dental students in this limited pilot study were more consistent when viewing a repeat slide using the 3-point scale

The Effect of Smoking and Brushing on the Color Stability and Stainability of Different CAD/CAM Restorative Materials

This study aimed to investigate and compare the color stability and stainability of computer-aided design/computer-aided manufacturing (CAD/CAM) restorative materials in their glazed (G) and polished (P) state when exposed to cigarette smoke, as well as after brushing. Three CAD/CAM restorative materials were investigated: lithium disilicate CAD (LD), zirconia (Zr), and Telio PMMA CAD (PMMA), according to their surface finishing and assignment to cigarette smoking exposure or soaking in the saliva (control) group. The color change (∆E) was calculated before and after the intervention performed for all specimens, using the L*a*b values to quantitatively assess the shade differences. Statistical analysis was performed using one-way repeated measures ANOVA and Bonferroni multiple comparison analysis (α = 0.05). The surface finishing did not influence the materials’ stainability. Color change was noted after smoking, LD and Zr-G and Zr-P had a comparable color change (p > 0.05), while PMMA presented lower ∆E values (p < 0.05). After brushing, all specimens had a significant color change that was high for LD-G and LD-P, and Zr-G, compared with Zr-P and PMMA (p < 0.05). In conclusion, the exposure to cigarette smoke showed that LD, Zr, and PMMA are all susceptible to staining, but brushing decreases surface staining

Evaluation of a two-step pouring technique for implant-supported prostheses impression

The aim of this study was to evaluate the efficacy of a pouring technique for implant-supported prostheses impressions. A metallic matrix (control group) with two implants positioned at 90 and 65 degrees was fabricated. The matrix was submitted to the direct transfer impression technique. In group CP (conventional pouring - n = 10), casts were obtained by the conventional pouring technique. In group EP (experimental pouring - n = 10), the analogs were embraced with latex tubes before the first pouring and then submitted to a second pouring. Vertical misfit and implants/analogs inclinations were evaluated. Data were analyzed by analysis of variance and Tukey's test (p < .05). Results demonstrated significant difference (p < .05) between control and experimental groups for misfit measurement in perpendicular implant/analog and between control group and group EP in leaning implant/analog. Considering inclination, there were significant differences (p < .05) between control and experimental groups for leaning analogs. Independently of the pouring technique, perpendicular implants produced more accurate casts

The Potential Role of a Surface-Modified Additive-Manufactured Healing Abutment on the Expression of Integrins &alpha;2, &beta;1, &alpha;v, and &beta;6 in the Peri-Implant Mucosa: A Preliminary Human Study

The stability of peri-implant soft tissues is essential for long-term success. Integrins play a vital role in biological processes through developing and maintaining cell interactions; however, few studies have evaluated the effects of modifications to abutment surfaces on cell adhesion across integrin expression. Therefore, this pilot study assessed the influence of different surface topographies of titanium healing abutments prepared by additive manufacturing (AM) on the gene expression levels of the integrin subunits &alpha;2, &beta;1, &alpha;v, and &beta;6 in the human peri-implant mucosa. Thirteen healthy adults were included. Depending on the number of required implants, the subjects were distributed in different groups as a function of healing abutment topography: group 1 (fully rough surface); group 2 (upper machined + lower rough); group 3 (rough upper surface + lower machined); group 4 (fully machined). A total of 40 samples (n = 10/group) of the peri-implant mucosa around the abutments were collected 30 days after implant placement, and subsequently, the gene expression levels were evaluated using real-time PCR. The levels of gene expression of &beta;1-subunit integrin were upregulated for individuals receiving fully rough surface abutments compared with the other surface topographies (p &lt; 0.05). However, the healing abutment topography did not affect the gene expression levels of the &alpha;2, &alpha;v, and &beta;6 integrin subunits in the human peri-implant mucosa (p &gt; 0.05). This preliminary study suggested that controlled modifications of the surface topography of titanium healing abutments produced by AM may influence the quality of the peri-implant mucosa in the early stages of the soft tissue healing process

Mapping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coating

Chemical and topographical surface modifications on dental implants aim to increase the bone surface contact area of the implant and improve osseointegration. This study analyzed the cellular response of undifferentiated mesenchymal stem cells (MSC), derived from senile rats’ femoral bone marrow, when cultured on a bioactive coating (by plasma electrolytic oxidation, PEO, with Ca2+ and P5+ ions), a sandblasting followed by acid-etching (SLA) surface, and a machined surface (MSU). A total of 102 Ti-6Al-4V discs were divided into three groups (n = 34). The surface chemistry was analyzed by energy dispersive spectroscopy (EDS). Cell viability assay, gene expression of osteoblastic markers, and mineralized matrix formation were investigated. The cell growth and viability results were higher for PEO vs. MSU surface (p = 0.001). An increase in cell proliferation from 3 to 7 days (p p p = 0.001) among groups. In addition, the PEO surface showed a higher mineralized matrix bone formation (p = 0.003). In conclusion, MSC from senile female rats cultured on SLA and PEO surfaces showed similar cellular responses and should be considered for future clinical investigations

Surface-treated commercially pure titanium for biomedical applications: electrochemical, structural, mechanical and chemical characterizations

Modified surfaces have improved the biological performance and biomechanical fixation of dental implants compared to machined (polished) surfaces. However, there is a lack of knowledge about the surface properties of titanium (Ti) as a function of different surface treatment. This study investigated the role of surface treatments on the electrochemical, structural, mechanical and chemical properties of commercial pure titanium (cp-Ti) under different electrolytes. Cp-Ti discs were divided into 6 groups (n = 5): machined (M—control); etched with HCl + H2O2 (Cl), H2SO4 + H2O2 (S); sandblasted with Al2O3 (Sb), Al2O3 followed by HCl + H2O2 (SbCl), and Al2O3 followed by H2SO4 + H2O2 (SbS). Electrochemical tests were conducted in artificial saliva (pHs 3; 6.5 and 9) and simulated body fluid (SBF—pH 7.4). All surfaces were characterized before and after corrosion tests using atomic force microscopy, scanning electron microscopy, energy dispersive microscopy, X-ray diffraction, surface roughness, Vickers microhardness and surface free energy. The results indicated that Cl group exhibited the highest polarization resistance (Rp) and the lowest capacitance (Q) and corrosion current density (Icorr) values. Reduced corrosion stability was noted for the sandblasted groups. Acidic artificial saliva decreased the Rp values of cp-Ti surfaces and produced the highest Icorr values. Also, the surface treatment and corrosion process influenced the surface roughness, Vickers microhardness and surface free energy. Based on these results, it can be concluded that acid-etching treatment improved the electrochemical stability of cp-Ti and all treated surfaces behaved negatively in acidic artificial saliva65251261FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP2013/24112-2; 2013/08451-

The role of nicotine, cotinine and caffeine on the electrochemical behavior and bacterial colonization to cp-Ti

Although smoking promotes deleterious effect to bone healing, there is a lack of study investigating its role on the implant structure and biofilm growth. We hypothesized that nicotine, cotinine and caffeine would impair the corrosion resistance of commercially-pure titanium (cp-Ti) and would enhance Streptococcus sanguinis biofilm growth. Neither the smoking products nor the caffeine affected the corrosion tendency (P>.05) and the oxide layer resistance (P=.762) of cp-Ti. Lower capacitance values were noted in the presence of nicotine (P=.001) and cotinine (P=.0006). SEM showed no pitting corrosion, and the EDS spectra did not differ among groups. Nicotine (300μg/mL) induced higher surface roughness (P=.03) and greater surface change of cp-Ti. Nicotine at 3μg/mL, and cotinine at 0.3 and 3μg/mL increased the number of viable cells (P.05). These findings suggest a greater biofilm accumulation in smokers, a risk factor that may lead to peri-implantitis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP