Research on Curriculum Design of Internet of Things Major in Higher Vocational Colleges based on “Integration of courses and Competitions”

Competition course integration mode is a new teaching mode, which combines the traditional competition course mode and integration teaching mode, aiming at improving students’ learning eff ect and ability. Through the introduction and analysis of the existing problems of the competition course integration mode, this paper discusses its application in the course design of “competition course integration” for the Internet of Things major in higher vocational colleges, including the course design objectives, contents, evaluation methods and so on. The research shows that the competition course integration mode has great application potential in higher vocational education, which can effectively improve students’ learning interest and learning effect, and promote the improvement of students’ comprehensive quality and ability

Long-term open-water season stream temperature variations and changes over Lena River Basin in Siberia

This study systematically analyzes long-term (1950-1992) stream temperature records for the major sub-basins within the Lena River watershed in order to describe water temperature regimes over the various parts of the Lena watershed and document significant stream temperature changes induced by reservoir regulation, and by natural variations/changes. The results show that the open water season can be divided into three consecutive stages---"increasing temperature stage" in the early open water season, "stable temperature stage" in the mid-warm season, and "decreasing temperature stage" in the late open water season. Temperature conditions are similar over the Aldan and Upper Lena regions. However, stream temperatures at the Lena basin outlet are up to 8 °C lower than those over the southern sub-basins. This suggests that the latitudinal difference in climatic variables, such as air temperature, might be the major control on stream temperature regime. Results also demonstrate that the reservoir regulation has a strong influence on the regional water temperature regime and change in the regulated sub-basin. Reservoir regulation has increased (decreased) the downstream water temperatures in the Vilui valley during the early (mid) open water season. Trend analyses show consistent warming trends across the entire Lena River basin in the early open water season. This may indicate a response to earlier snowmelt over the Lena River watershed. Trend results also demonstrate that the Aldan tributary, without much human impact, experiences warming (cooling) trends in the first (second) half of the open water season, leading to a stream temperature regime shift toward early open water season. The upper Lena River has warming (cooling) trends in the early (mid-late) open water season. Over the regulated Vilui tributary, however, stream temperatures have significantly increased in the early and late parts of the warm season due to combined effects of natural changes and reservoir regulation. Over the Lena basin as a whole, strong positive correlations have been found between the basin mean monthly air and water temperatures during the warm season. Increasing water temperatures were observed during the early and mid-June. Because of stream temperature increase in this peak flow period, the Lena River heat flux has gone up by 23% in June. This may have considerable impact on the thermal conditions of the Laptev Sea in the early summer season

A New Liquid Chromatography/Tandem Mass Spectrometry Method for Quantification of Gangliosides in Human Plasma

Gangliosides are a family of glycosphingolipids characterized by mono- or polysialic acid-containing oligosaccharides linked through 1,3- and 1,4-β glycosidic bonds with subtle differences in structure that are abundantly present in the central nervous systems of many living organisms. Their cellular surface expression and physiological malfunction are believed to be pathologically implicated in considerable neurological disorders, including Alzheimer and Parkinson diseases. Recently, studies have tentatively elucidated that mental retardation or physical stagnation deteriorates as the physiological profile of gangliosides becomes progressively and distinctively abnormal during the development of these typical neurodegenerative syndromes. In this work, a reverse-phase liquid chromatography/tandem mass spectrometry (LC/MS/MS) assay using standard addition calibration for determination of GM2, GM3, GD2, and GD3 in human plasma has been developed and validated. The analytes and internal standard were extracted from human plasma using a simple protein precipitation procedure. Then the samples were analyzed by reverse-phase ultra-performance liquid chromatography (UPLC)/MS/MS interfaced to mass spectrometry with electrospray ionization using a multiple reaction monitoring mode to obtain superior sensitivity and specificity. This assay was validated for extraction recovery, calibration linearity, precision, and accuracy. Our quick and sensitive method can be applied to monitor ganglioside levels in plasma from normal people and neurodegenerative patients

Oxygen Reduction Activity and Stability of Composite Pdx/Co-Nanofilms/C Electrocatalysts in Acid and Alkaline Media

The morphology tuning of Pd and Pd-M nanoparticles is one of the significant strategies to control the catalytic activity toward oxygen reduction reaction (ORR). In this study, composite Pdx/Co-nanofilms/C electrocatalysts of Pd nanoparticles implanted onto Co nanofilms were synthesized on an immiscible ionic liquid (IL)/water interface for ORR. The Pd nanoparticles implanted onto Co nanofilms show a marked distortion of crystal lattice and surface roughness. These Pdx/Co-nanofilms/C electrocatalysts exhibit enhanced activity for ORR compared with Pd/C and PdxCo/C catalysts in both acid and alkaline solutions, in which the Pd3/Co-nanofilms/C catalyst displays the highest ORR mass activity. The superior ORR mass activities of the fabricated Pdx/Co-nanofilms/C catalysts may be mainly attributed to their larger catalytic areas, which are conferred by the rough surface of Pd nanoparticles with a distorted crystal lattice, and the synergistic effect between the surface Pd atoms and the 2D Co nanofilm substrate. The relationship between ORR mass activity and Pd/Co atom ratio varies in different electrolytes. Furthermore, by using proper heat-treatment methods, the Pdx/Co-nanofilms/C catalysts exhibit improved cycling stability compared with pure Pd/C catalyst after extended potential cycling

High level soluble expression, one-step purification and characterization of HIV-1 p24 protein

<p>Abstract</p> <p>Background</p> <p>P24 protein is the major core protein of HIV virus particle and has been suggested as a specific target for antiviral strategies. Recombinant p24 protein with natural antigenic activity would be useful for various studies, such as diagnostic reagents and multi-component HIV vaccine development. The aim of this study was to express and purify the p24 protein in soluble form in <it>E.coli</it>.</p> <p>Results</p> <p>According to the sequence of the p24 gene, a pair of primers was designed, and the target sequence of 700 bp was amplified using PCR. The PCR product was cloned into pQE30 vector, generating the recombinant plasmid pQE30-p24. SDS-PAGE analysis showed that the His-tagged recombinant p24 protein was highly expressed in soluble form after induction in <it>E. coli </it>strain BL21. The recombinant protein was purified by nickel affinity chromatography and used to react with HIV infected sera. The results showed that the recombinant p24 protein could specifically react with the HIV infected sera. To study the immunogenicity of this soluble recombinant p24 protein, it was used to immunize mice for the preparation of polyclonal antibody. Subsequent ELISA and Western-Blot analysis demonstrated that the p24 protein had proper immunogenicity in inducing mice to produce HIV p24 specific antibodies.</p> <p>Conclusion</p> <p>In this work, we report the high level soluble expression of HIV-1 p24 protein in <it>E. coli</it>. This soluble recombinant p24 protein specifically react with HIV infected sera and elicit HIV p24 specific antibodies in mice, indicating this soluble recombinant p24 protein could be a promising reagent for HIV diagnosis.</p

Synthesis of nanoflower-shaped MXene derivative with unexpected catalytic activity for dehydrogenation of sodium alanates.

Surface group modification and functionalization of two-dimensional materials in many cases are deemed as effective approaches to achieve some distinctive properties. Herein, we present a new nanoflower-shaped TiO2/C composite which was synthesized by in situ alcoholysis of two-dimensional layered MXene (Ti3C2(OHxF1-x)2) in a dilute HF solution (0.5 wt %) for the first time. Furthermore, it is demonstrated that it bestows a strong catalytic activity for the dehydrogenation of NaAlH4. The results show that the NaAlH4 containing 10 wt % A0.9R0.1-TiO2/C (containing 90% anatase TiO2 and 10% rutile TiO2) composite merely took ∼85 min to reach a stable and maximum dehydrogenation capacity of ∼3.08 wt % at 100 °C, and it maintains stable after ten cycles, which is the best Ti-based catalyst for the dehydrogenation of NaAlH4 reported so far. Theoretical calculation confirms that this C-doping TiO2 crystals remarkably decreases desorption energy barrier of Al-H bonding in NaAlH4, accelerating the breakdown of Al-H bonding. This finding raises the potential for development and application of new fuel cells

Research Progress in the Formation of Quality of Dry-Aged Beef and Approaches for Its Added Value

Dry aging is an effective method to improve the quality and value of meat. Meat quality characteristics such as tenderness and flavor can be significantly improved through a series of biochemical reactions. In recent years, dry-aged beef has gained huge popularity among consumers and research attention due to its unique flavor. However, there are some problems with the dry-aged beef industry, such as large quality differences, high losses during maturation, and high production costs. This article systematically summarizes quality characteristics of dry-aged beef, including flavor, tenderness, safety, color and water-holding capacity (WHC). The mechanism of formation of its characteristic flavor is briefly described. The effects of different dry-aging conditions on beef quality are summarized. New dry aging technologies and value-added ways to reuse the crust are introduced. This article will hopefully provide theoretical guidance for the standardized, efficient and high-value development of the dry-aged beef industry