HYDRODYNAMICS OF A SPOUT-FLUID BED WITH AN ENLARGED COLUMN FOR FINE PARTICLES

In order to improve the spoutability for fine particles, a new type of spout-fluid bed with an enlarged column section was proposed. Comparison of flow regime maps for the new design with a conventional spout-fluid bed showed that the range of stable spouting regime was extended when an enlarged column section was employed

Development of SCAR Marker Related to Summer Stress Tolerance in Tall Fescue (Festuca arundinacea)

Summer stress tolerance (SST) is one of the most important breeding objectives in tall fescue (Festuca arundinacea), an important perennial cool-season grass. However, breeding for better SST is generally complicated by the many environmental factors involved during the growing season. Utilizing the bulked segregant analysis (BSA), we were able to identify one marker related to SST from 100 inter-simple sequence repeat (ISSR) markers and 800 random amplified polymorphic DNA (RAPD) markers, and successfully developed a dominant sequence characterized amplified region (SCAR) marker T_SC856 from the UBC856 sequence. Furthermore, the SCAR marker was tested in different clones of new populations, which were identified under complex summer stress (high temperature and humidity, Pythium blight, and brown patch), and it exhibited relatively high consistency (77%) with the phenotype. We believe that with more markers obtained in the future, better efficiency is likely to be achieved in breeding for improved SST in tall fescue and possibly other species as well. Further studies that analyze the factors relating to the SCAR marker are needed

Scaling Relationships of Gas-Solid Spouted Beds

An additional parameter, the particle-particle coefficient of restitution was added to the scaling relationship of spouted beds proposed by He et al. (4) by granular kinetic theory analysis of the particle movements in both spout and annulus regions. The experimental verification was conducted in two spouted beds with 80 and 120 mm ID. It has been found that good similarity could not be obtained when the coefficients of restitution were not matched

Characterization of Diversity and Probiotic Efficiency of the Autochthonous Lactic Acid Bacteria in the Fermentation of Selected Raw Fruit and Vegetable Juices

The diversity of indigenous lactic acid bacteria (LAB) in fermented broccoli, cherry, ginger, white radish, and white-fleshed pitaya juices was analyzed using culture-independent and -dependent approaches. The major properties of selected probiotic strains, including dynamic variations in pH, viable cell counts, antibiotic resistance, bacterial adhesion to hydrophobic compounds, and survivability during simulated gastrointestinal transit, were investigated using broccoli as the fermentation substrate. In broccoli and ginger juices, the genus Lactobacillus occupied the dominant position (abundances of 79.0 and 30.3%, respectively); in cherry and radish juices, Weissella occupied the dominant position (abundances of 78.3 and 83.2%, respectively); and in pitaya juice, Streptococcus and Lactococcus occupied the dominant positions (52.2 and 37.0%, respectively). Leuconostoc mesenteroides, Weissella cibaria/soli/confusa, Enterococcus gallinarum/durans/hirae, Pediococcus pentosaceus, Bacillus coagulans, and Lactococcus garvieae/lactis subspecies were identified by partial 16S rRNA gene sequencing. In general, the selected autochthonous LAB isolates displayed no significant differences in comparison with commercial strains with regard to growth rates or acidification in fermented broccoli juice. Among all the isolates, L. mesenteroides B4-25 exhibited the highest antibiotic resistance profile (equal to that of L. plantarum CICC20265), and suitable adhesion properties (adhesion of 13.4 ± 5.2% ∼ 36.4 ± 3.2% and 21.6 ± 1.4% ∼ 69.6 ± 2.3% to ethyl acetate and xylene, respectively). Furthermore, P. pentosaceus Ca-4 and L. mesenteroides B-25 featured the highest survival rates (22.4 ± 2.6 and 21.2 ± 1.4%, respectively), after simulated gastrointestinal transit. These results indicated a high level of diversity among the autochthonous bacterial community in fermented fruit and vegetable juices, and demonstrated the potential of these candidate probiotics for applications in fermentation

Human pluripotent stem cell-derived epicardial progenitors can differentiate to endocardial-like endothelial cells.

During heart development, epicardial progenitors contribute various cardiac lineages including smooth muscle cells, cardiac fibroblasts, and endothelial cells. However, their specific contribution to the human endothelium has not yet been resolved, at least in part due to the inability to expand and maintain human primary or pluripotent stem cell (hPSC)-derived epicardial cells. Here we first generated CDH5-2A-eGFP knock-in hPSC lines and differentiated them into self-renewing WT1+ epicardial cells, which gave rise to endothelial cells upon VEGF treatment in vitro. In addition, we found that the percentage of endothelial cells correlated with WT1 expression in a WT1-2A-eGFP reporter line. The resulting endothelial cells displayed many endocardium-like endothelial cell properties, including high expression levels of endocardial-specific markers, nutrient transporters and well-organized tight junctions. These findings suggest that human epicardial progenitors may have the capacity to form endocardial endothelium during development and have implications for heart regeneration and cardiac tissue engineering

Projection of future climate change in the Poyang Lake Basin of China under the global warming of 1.5–3°C

This study projected the future climate changes in the Poyang Lake Basin (PLB) of China under various global warming targets (1.5–3°C), based on the latest Coupled Model Intercomparison Project Phase 6 (CMIP6) and 4 statistical downscaling methods, including Quantile Mapping (QM), Daily Translation (DT), Delta, and Local Intensity Scaling (LOCI). The RMSE, R$^{2}$ and KGE indicators were used to evaluate the competency of the aforementioned methods applied to daily precipitation (Pre), daily mean temperature (Tas), daily maximum temperature (Tasmax), and daily minimum temperature (Tasmin). The global warming of 1.5, 2 and 3°C will occur around 2040, from 2045 to 2080 and around 2075, respectively, for the emission scenarios of SSP1-2.6, SSP2-4.5, SSP3-7.0 and SSP5-8.5. The results demonstrated that under the 1.5, 2 and 3°C global warming targets, the projected annual precipitation declined by 14.82, 11.92 and 8.91% relative to the reference period (1986–2005), respectively. The Tas increased significantly by 0.43, 0.94 and 1.92°C and the Tasmax increased by 0.58, 1.11 and 2.09°C. The Tasmin decreased by 0.29°C under the 1.5°C warming target, while it increased by 0.19 and 1.18°C under the 2 and 3°C warming targets. The spatial distributions of future annual precipitation in the PLB were relative consistent. However, the regional variability was significant, which the southern and eastern regions experienced more precipitation than the northern and western regions. The south-central part of the Ganjiang basin was the high-value area while the northeastern part was the low-value area. The Tas, Tasmax and Tasmin had a consistent spatial variation characteristic that the high latitude areas were warmer than the low latitude areas, and the western regions were warmer than the central and eastern regions while the northeastern regions were cooler than the remaining regions

Spatial and temporal heterogeneity of tropical cyclone precipitation over China from 1959 to 2018

Tropical cyclone precipitation (TCP) can cause serious floods and urban waterlogs as well as cause various secondary disasters, such as landslides and debris flows, which negatively affect human lives and the sustainable development of the economy. This study applied the prewhitening Mann-Kendall test, empirical orthogonal function, and continuous wavelet transform to investigate the long-term trend, spatiotemporal pattern, and periodicity of TCP at monthly, interannual, and interdecadal timescales over China. The recurrence risks of extreme TCP were analyzed using the return period estimation model. The results showed that 1) TCP displayed a significant increasing trend, especially in eastern China, inland areas, and Guangxi Province. The TCP periodicities were 2.5 and 4.9 years across all of China. However, TCP cycles had large discrepancies in the time and frequency domains in different subregions. 2) Monthly TCP demonstrated a decreasing trend in May and an increasing trend from June to October in all of China. The TCP in northeastern China and southern China tended to decrease in July and August, respectively. 3) TCP demonstrated a decreasing tendency from the 1960s–1980s followed by a rebounding trend in the 1990s–2010s. In addition, TCP showed a dipole mode in the 1970s and 2000s. 4) There was an increasing recurrence risk of extreme TCP in the Yangtze River Delta, Hainan Province, southeastern Guangxi Province, and southwestern Guangdong Province. It is therefore necessary to improve forecasting of extreme TCP events to improve risk management and prevention capacity of natural disasters, especially in regions with high population and economy exposure

Efficient Differentiation of Human Pluripotent Stem Cells to Endothelial Progenitors via Small-Molecule Activation of WNT Signaling

SummaryHuman pluripotent stem cell (hPSC)-derived endothelial cells and their progenitors may provide the means for vascularization of tissue-engineered constructs and can serve as models to study vascular development and disease. Here, we report a method to efficiently produce endothelial cells from hPSCs via GSK3 inhibition and culture in defined media to direct hPSC differentiation to CD34+CD31+ endothelial progenitors. Exogenous vascular endothelial growth factor (VEGF) treatment was dispensable, and endothelial progenitor differentiation was β-catenin dependent. Furthermore, by clonal analysis, we showed that CD34+CD31+CD117+TIE-2+ endothelial progenitors were multipotent, capable of differentiating into calponin-expressing smooth muscle cells and CD31+CD144+vWF+I-CAM1+ endothelial cells. These endothelial cells were capable of 20 population doublings, formed tube-like structures, imported acetylated low-density lipoprotein, and maintained a dynamic barrier function. This study provides a rapid and efficient method for production of hPSC-derived endothelial progenitors and endothelial cells and identifies WNT/β-catenin signaling as a primary regulator for generating vascular cells from hPSCs