Feto maternal outcome in obstructed labor: a tertiary centre study

Background: The number of maternal deaths as a result of obstructed labor is 8% globally but this number varies in developing country, it ranges 4-70% of all maternal deaths and it is also associated to high perinatal mortality rate. Objective of the study was to find out the proportion of obstructed labour cases and their feto-maternal outcome during last 3 years at tertiary level institute.Methods: A cross sectional observation study was done at Department of Obstetrics and Gynecology, People’s College of Medical Sciences and Research Centre Bhopal. All pregnant women presenting with obstructed labor who delivered at our hospital during last 3 years duration (January 2015 to December 2017) were studied for their feto -maternal outcome.Results: 53% cases had duration of trail more than 16 hours. 84% were referred from primary health centers of nearby rural areas. All  cases  of obstructed labor  delivered by cesarean section (100%). 44% were primigravida. 72% of cases had Cephalopelvic disproportion as the cause. 28% of cases had longer stay more than 7 days at hospital. 32% had  fever  during post operative period 12.5% cases had wound sepsis and 6% of cases required re-suturing of wound during post operative period. 72% baby's birth weight was between 3 to 3.4 kg. 94% of the babies survived where as 6% of babies were still birth. 16% of babies born to obstructed labor mother had APGAR less than 7 at 5 minutes of birth. 6% fetus were IUFD.Conclusions: Cephalopelvic disproportion was the most common cause for obstructed labor. Timely identification of prolonged labor and timely referral and management at place where operation theatre, NICU and blood bank facilities are available can save the life of both baby and mother

An inhibitor of the mitotic kinase, MPS1, is selective towards pancreatic cancer cells

Indiana University-Purdue University Indianapolis (IUPUI).The abysmal five year pancreatic cancer survival rate of less than 6% highlights the need for new treatments for this deadly malignancy. Cytotoxic drugs normally target rapidly dividing cancer cells but unfortunately often target stem cells resulting in toxicity. This warrants the development of compounds that selectively target tumor cells. An inhibitor of the mitotic kinase, MPS1, which has been shown to be more selective towards cancer cells than non-tumorigenic cells, shows promise but its effects on stem cells has not been investigated. MPS1 is an essential component of the Spindle Assembly Checkpoint and is proposed to be up-regulated in cancer cells to maintain chromosomal segregation errors within survivable limits. Inhibition of MPS1 kinase causes cancer cell death accompanied by massive aneuploidy. Our studies demonstrate that human adipose stem cells (ASCs) and can tolerate higher levels of a small molecule MPS1 inhibitor than pancreatic cancer cells. In contrast to PANC-1 cancer cells, ASCs and telomerase-immortalized pancreatic ductal epithelial cells did not exhibit elevated chromosome mis-segregation after treatment with the MPS1 inhibitor for 72hrs. In contrast, PANC-1 pancreatic cancer cells exhibited a large increase in chromosomal mis-segregation under similar conditions. Furthermore, growth of ASCs was minimally affected post treatment whereas PANC-1 cells were severely growth impaired suggesting a favorable therapeutic index. Our studies, demonstrate that MPS1 inhibition is selective towards pancreatic cancer cells and that stem cells are less affected in vitro. These data suggest MPS1 inhibition should be further investigated as a new treatment approach in pancreatic cancer

Use of an Episodic Food Intake Monitoring System to Evaluate Feeding Behavior in Mice

poster abstractThe measurement of food consumption in laboratory animals is critical to studies in metabolism and obesity. Unfortunately, feeding behavior is very sensitive to the environment. Many factors such as the change of cages, diet, and human interactions can introduce undesired experimental variation. Here we describe our experiences with a commercially available episodic food intake monitoring system, the BioDAQ Monitor. This system is designed to quantitatively record feeding behavior in mice. It continuously monitors the weight of the food and uses this information to determine bout length and size. Bouts that occur soon after one another can then be defined as meals. When an animal jostles the food hopper while eating, the weight of the hopper fluctuates and eating is considered to be in progress. Once the hopper weight has been stable for a specified time, that period of feeding is considered to be concluded. The system also has the capability to assess either food or liquid choice paradigms and to directly measure the administration of orally available drugs in either the feed or the water. In addition to these functions, the system uses an environment monitor to record temperature, humidity and lighting of the room every five minutes. Here we present data showing measurements taken in hyperphagic mutant mice, altered feeding paradigms, and under different drug and protein hormone treatments. Future studies using this system will continue to focus on the hyperphagia associated obesity phenotype observed in mice upon conditional disruption of primary cilia

Significance of Correct Diagnosis of Odontogenic Extraoral Sinus: A Report Of Two Cases

Cutaneous draining sinus tracts of odontogenic origin often are a diagnostic challenge. A delay in correctly diagnosing these types of lesions can result in unnecessary antibiotic therapy and surgical treatment. This case report presents the clinical course of two cases with extra-oral sinus tract formation, from diagnosis and treatment to short-term follow-up and evaluation. These facial lesions were initially misdiagnosed as lesions of non-odontogenic origin. Later on an odontogenic cause was identified and endodontic intervention resulted in resolution of the problem, confirming the initial misdiagnosis