464 research outputs found
Liposomes: A targeted drug delivery system- A review
Liposomes are a novel drug delivery system (NDDS), which are vesicular structures consisting of hydrated bilalyers which form spontaneously whenphospholipids are dispersed in water. They are simple microscopic vesicles in which an aqueous volume is entirely enclosed by a membrane composed of lipid bilayers. Novel drug delivery system aims to deliver the drug at a rate directed by the needs of the body during the period of treatment, and channel the active entity to the siteof action. It has been a study interest in the development of a NDDS. Liposomes are colloidal spheres of cholesterol non-toxic surfactants, sphingolipids, glycolipids, long chain fatty acids and even membrane proteins and drug molecules or it is also called vesicular system. It is differ in size, composition and charge. It is a drug carrier loaded with great variety of molecules such as small drug molecules, proteins, nucleotides and even plasmids. Few drugs are also formulated as liposomes to improve their therapeutic index. Consequently a number of vesicular drug delivery systems such as liposomes, niosomes, transfersomes, and pharmacosomes were developed. The focus of this review is to the various method of preparation, characterization of liposomes, advantages and applications etc
Ionophore-mediated transmembrane movement of divalent cations in small unilamellar liposomes: an evaluation of the chlortetracycline fluorescence technique and correlations with black lipid membrane studies
Conceptual advances in the field of membrane transport have, in the main, utilized artificial membranes, both planar and vesicular. Systems of biological interest, viz., cells and organelles, resemble vesicles in size and geometry. Methods are, therefore, required to extend the results obtained with planar membranes to liposome systems. In this report we present an analysis of a fluorescence technique, using the divalent cation probe chlortetracycline, in small, unilamellar vesicles, for the study of divalent cation fluxes. An ion carrier (X537 A) and a pore former (alamethicin) have been studied. The rate of rise of fluorescence signal and the transmembrane ion gradient have been related to transmembrane current and potential, respectively. A second power dependence of ion conduction-including the electrically silent portion thereof - on X537 A concentration, has been observed. An exponential dependence of "current" on "transmembrane potential" in the case of alamethicin is also confirmed. Possible errors in the technique are discussed
ΠΠ»ΠΈΡΠ½ΠΈΠ΅ Ρ Π»ΠΎΡΠΈΠ΄Π° Π°Π»ΡΠΌΠΈΠ½ΠΈΡ ΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΊΡΠΈΠΎΠΏΡΠΎΡΠ΅ΠΊΡΠΎΡΠΎΠ² Π½Π° ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ Π³ΠΈΠΏΠ΅ΡΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΡΠΈΠΎΠ³Π΅ΠΌΠΎΠ»ΠΈΠ·Π° ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
Niosome: A Novel Drug Delivery System
The concept of drug targeting or site specific drug delivery was introduced first time by Paul Elrich in 1909, when he reported magic bullet to deliver a drug to the desired site of action without affecting the non target organs or tissues (Juliano, 1980) by associating the drug with a pharmacologically inactive carrier capable of conveying the drug selectively towards its target cells. The main goal of a site specific drug delivery system is not only to increase the selectivity and drug therapeutic index, but also to reduce the toxicity of the drug
Evaluation of liposomes coated with a pH responsive polymer
Liposomes have been coated with the pH responsive polymer, Eudragit S100, and the formulation's potential for lower gastrointestinal (GI) targeting following oral administration assessed. Cationic liposomes were coated with the anionic polymer through simple mixing. The evolution of a polymer coat was studied using zeta potential measurements and laser diffraction size analysis. Further evidence of an association between polymer and liposome was obtained using light and cryo scanning electron microscopy. Drug release studies were carried out at pH 1.4, pH 6.3 and pH 7.8, representing the pH conditions of the stomach, small intestine and ileocaecal junction, respectively.\ud
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The polymer significantly reduced liposomal drug release at pH 1.4 and pH 6.3 but drug release was equivalent to the uncoated control at pH 7.8, indicating that the formulation displayed appropriate pH responsive release characteristics. While the coating layer was not able to withstand the additional challenge of bile salts this reinforces the importance of evaluating these types of formulations in more complex media.\ud
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ΠΡΠΈΠΎΠΏΡΠΎΡΠ΅ΠΊΡΠΈΡ ΠΏΠΎΠ»ΠΈΡΡΠΈΠ»Π΅Π½Π³Π»ΠΈΠΊΠΎΠ»Π΅ΠΌ ΠΈ Π΄Π΅ΠΊΡΡΡΠ°Π½ΠΎΠΌ ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ²
Molecular Weight Dependence of Polymersome Membrane Elasticity and Stability
Vesicles prepared in water from a series of diblock copolymers and termed
"polymersomes" are physically characterized. With increasing molecular weight
, the hydrophobic core thickness for the self-assembled bilayers
of polyethyleneoxide - polybutadiene (PEO-PBD) increases up to 20 -
considerably greater than any previously studied lipid system. The mechanical
responses of these membranes, specifically, the area elastic modulus and
maximal areal strain are measured by micromanipulation. As expected
for interface-dominated elasticity, ( 100 ) is found to be
independent of . Related mean-field ideas also predict a limiting
value for which is universal and about 10-fold above that typical of
lipids. Experiments indeed show generally increases with
, coming close to the theoretical limit before stress relaxation is
opposed by what might be chain entanglements at the highest . The
results highlight the interfacial limits of self-assemblies at the nano-scale.Comment: 16 pages, 5 figures, and 1 tabl
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