Bioavailability, metabolism and antioxidative defence of polyphenols in different organs of C57BL/6 mice

U ovome radu je istražena in vivo farmakokinetika-apsorpcija, metabolizam i raspodjela polifenolnih spojeva iz vodenog ekstrakta cvijeta trnine (Prunus spinosa L.), te vodenog ekstrakta cvijeta trnine u kombinaciji s prehranom obogaćenom proteinima, mastima i ugljikohidratima u organima (tanko crijevo, jetra, mozak, bubreg) miša C57BL/6. Također je istražen bioakumulacijski potencijal nakupljanja molekula polifenola u navedenim organima, te biološki raspoložive maksimalne koncentracije pojedinih skupina molekula polifenola. Osim navedenog, istraženi su glavni biomarkeri oksidacijskog stresa (MDA, PC), aktivacija antioksidacijskih enzima (SOD, GSH, CAT) i antioksidacijski kapaciteti navedenih tkiva. Dobiveni rezultati pokazuju da se od ukupno 32 vrste polifenolnih spojeva koje nalazimo u ekstraktu cvijeta trnine, apsorpcijom i bioraspodjelom po organima u različitim vremenima bioakumuliraju različite vrste polifenolnih spojeva što potvrđuje postavljenu hipotezu o selektivnoj preferenciji za bioakumulacijom i zadržavanjem pojedinog tipa polifenolnih spojeva unutar različitih organa. Rezultati analize antioksidacijskog sustava su također pokazali koje pojedine skupine polifenola i njihovih metabolita aktiviraju enzime uključene u antioksidacijski sustav obrane (SOD, GSH, CAT), a izmjereni antioksidacijski kapaciteti tkiva ukazuju da pojedini organi u pokusnim skupinama pokazuju relativno visoki antioksidacijski kapacitet u odnosu na kontrolnu skupinu životinja. Dobiveni rezultati ovog istraživanja pridonose boljem razumijevanju afiniteta pojedinih skupina polifenola prema pojedinim tkivima i organima s temeljnim ciljem postizanja boljeg antioksidacijskog statusa organizma.This thesis deals with the in vivo pharmacokinetics-absorption, metabolism and distribution of polyphenolic compounds from the sloe (Prunus spinosa L.) flower water extract and the water extract of sloe in combination with a diet enriched by proteins, fat and carbohydrates in the organs (small intestine, liver, brain, kidney) of the mouse C57BL/6. Furthermore, the bioaccumulative potential of polyphenolic compounds in these organs was also investigated, as well as the bioavailable maximum concentration of polyphenolic molecules. In addition, major oxidative stress biomarkers (MDA, PC), antioxidant enzyme activation (SOD, GSH, CAT), and antioxidative capacity of selected tissues were investigated. The obtained results show that out of a total of 32 types of polyphenolic compounds found in the sloe flower extract, various types of polyphenolic compounds bioaccumulate by absorption and biodistribution within organs at different times which confirms the set hypothesis of selective preference for bioaccumulation and retention of a certain type of polyphenolic compounds within different organs. The results of the antioxidation system analysis have also shown which groups of polyphenols and their metabolites activate specific enzymes involved in the antioxidative defence system (SOD, GSH, CAT) and the measured antioxidant tissue capacity indicates that certain organs in the test groups exhibit a relatively high antioxidative capacity in relation to the animal control group. The results of this study contribute to a better understanding of the affinity of certain groups of polyphenols to certain tissues and organs with the fundamental aim of achieving a better antioxidative status of the organism

Association between aryl hydrocarbon receptor and 4-hydroxynonenal in oxidative stress-mediated chronic rhinosinusitis with nasal polyps

Background: Сhronic rhinosinusitis with nasal polyps (CRSwNPs) is a distinct entity within the chronic rhinosinusitis group of diseases, which are chronic upper airway diseases with several pheno- and endotypes. Oxidative stress plays an important role in the pathogenesis of CRSwNPs. Aim: The aim was to assess the association between the expression of the aryl hydrocarbon receptor (AhR) and 4-hydroxynonenal (4-HNE) in patients with CRSwNPs. Methods: The study included 26 patients who underwent endoscopic sinus surgery – 14 patients with CRSwNPs, and 12 controls with healthy sinus mucosa. The expression of AhR and 4-HNE was assessed in tissue samples using immunohistochemistry. The level of 4-HNE in serum samples was measured using the ELISA assay. The total oxidative capacity (TOC) was assessed by measuring the peroxidase activity. Results: Higher levels of 4-HNE expression were observed in tissues (3, range 1-3 vs. 0, range 0-0, p<0.001) and serum (27.7±11.5 vs. 9.8±7.7 pmol/mg, p<0.001) samples of CRSwNPs patients, as compared to healthy controls. A higher expression of AhR was found in inflammatory cells (plasma cells, lymphocytes, eosinopholes) of CRSwNPs patients, compared to controls (3, range 1-3 vs. 2, range 1-2, p=0.001). There were no differences in TOC across groups (0.0285±0.0207 vs. 0.02978±0.0197 µM H2O2 eq., p=0.848). Patients with bronchial asthma (57%) had abundant eosinophiles in tissue samples. Patients with recalcitrant CRSwNPs had higher 4-HNE serum levels, compared to non-recalcitrant cases (27.3 vs. 24.2 pmol/mg, p=0.339). Conclusion: Patients suffering from CRSwNPs have oxidative stress mediated overexpression of AhR, which is linked to a chronic inflammatory response in the paranasal sinus tissues

Utjecaj ekstrakta cvijeta trnine na homeostazu glukoze u normoglikemijskom i aloksanom induciranom hiperglikemijskom C57BL/6 mišu

Research background. The use of plants and their extracts in treatments of chronic diseases is widely known in traditional medicine. The aim of this study is to determine the effects of 10-day consumption of blackthorn (Prunus spinosa L.) flower extract on blood glucose, glycaemic load, serum α-amlyase activity and insulin concentration in normoglycaemic and hyperglycaemic (alloxan-induced) mice model. Experimental approach. Normoglycaemic and hyperglycaemic (treated with alloxan, 150 mg per kg body mass) C57BL/6 mice were administered daily, during 10 days, blackthorn flower extract by gavage. The sugar mass concentration within the extract was determined by HPLC analysis. In mice, blood and serum blood glucose concentrations, and oral glucose tolerance test were determined by blood glucometer. Serum insulin concentration was determined by ELISA assay and α-amylase activity by colourimetric assay. Results and conclusions. The blackthorn flower extract increased glucose concentrations in normoglycaemic mice by 30% after the 1st and 5th day and by 17% after the 10th day of consumption. It is a consequence of released sugars because sugar analysis revealed 59.8 mg/L monosaccharides, mainly fructose (55.7 mg/L) and glucose (24.3 mg/L) in the extract. On the contrary, the extract consumption reduced serum blood glucose in hyperglycaemic mice by 29% after 10 days of treatment. Oral glucose tolerance test also confirmed that in the hyperglycaemic group treated with blackthorn flower extract glucose homeostasis was improved and showed decrease in blood glucose. Serum insulin concentration increased by 49% and serum α-amylase activity by 46% after 10 days of treatment with blackthorn flower extract in hyperglycaemic group. Thus, it can be concluded that blackthorn flower extract improved glucose tolerance, enhanced insulin secretion and lowered serum α-amylase activity. Novelty and scientific contribution. The obtained results show for the first time the potential of blackthorn (Prunus spinosa L.) flower extract in hyperglycaemia management.Pozadina istraživanja. Primjena biljaka i njihovih ekstrakata u liječenju kroničnih bolesti nadaleko je poznata u tradicionalnoj medicini. Svrha je ovoga rada bila utvrditi utjecaj desetodnevne konzumacije ekstrakta cvijeta trnine na koncentraciju glukoze u krvi, glikemijsko opterećenje, aktivnost α-amilaze i koncentraciju inzulina u serumu normoglikemijskih i hiperglikemijskih (induciranih aloksanom) miševa. Eksperimentalni pristup. Normoglikemijski i hiperglikemijski (inducirani s 150 mg aloksana po kg tjelesne mase) C57BL/6 miševi tretirani su tijekom 10 dana ekstraktom cvijeta trnine. Koncentracija šećera u ekstraktu određena je HPLC analizom, a koncentracija glukoze u krvi i oralna podnošljivost glukoze (oralni glukoza tolerans test) ispitane su glukometrom. Koncentracija inzulina u serumu određena je ELISA testom, a aktivnost α-amilaze kolorimetrijskom metodom. Rezultati i zaključci. Ekstrakt cvijeta trnine povećao je koncentraciju glukoze u krvi u normoglikemijskom mišu za 30 % nakon prvog i petog dana, te za 17 % nakon desetog dana konzumacije. To je bila posljedica prisustva slobodnih šećera, što je potvrđeno analizom njihovog sastava, kojom je utvrđeno da ekstrakt sadržava 59,8 mg/L monosaharida, uglavnom fruktoze (55,7 mg/L) i glukoze (24,3 mg/L). Suprotno tome, tretman ekstraktom cvijeta trnine je nakon 10 dana smanjio koncentraciju glukoze u krvi hiperglikemijskog miša za 29 %. Oralni glukoza tolerans test potvrdio je da se u hiperglikemijskoj skupini tretiranoj ekstraktom poboljšala podnošljivost glukoze te da je brže uspostavljena njezina homeostaza. Koncentracija inzulina u serumu povećala se za 49 %, a aktivnost α-amilaze za 46 % nakon desetodnevnog tretmana hiperglikemijske skupine ekstraktom cvijeta trnine. Može se zaključiti da ekstrakt cvijeta trnine poboljšava podnošljivost glukoze, potiče lučenje inzulina te smanjuje aktivnost serumske α-amilaze. Novina i znanstveni doprinos. Dobiveni rezultati prvi put pokazuju mogućnost primjene ekstrakta cvijeta trnine u regulaciji hiperglikemije

Polyphenol bioavailability and modulatory potential on brain antioxidative markers in C57BL/6 mouse

Background and purpose: Prunus spinose L. is a traditionally consumed, recently scientifically reexamined plant. Brain bioavailability and functionality of polyphenols (PPH) of blackthorn flower extract (PSE) was investigated.Materials and methods: C57BL/6 mice received oral daily repeated doses of 25 mg/kg body weight of total PSE polyphenols for 28 days. Brain concentrations of individual polyphenols from PSE were determined by UPLC/MS on 1st,7th, 14th, 21st, and 28th day. Brain antioxidative defense markers were examined as indicators of functionality after bioaccumulation.Results: A total of 68.7% PPH present in PSE were detected in the brain. Higher (p≤0.05) Cmax/AUClast in the PSE treatment vs. control group was recorded for 59.1% of brain detected compounds, indicating relatively good bioaccumulation in the brain. The highest present compounds in PSE were not necessarily the ones mostly bioapsorbed in the brain. Kaempherols were not significantly distributed, opposite to phenolic acids, quercetins or epicgaloatechin-3-gallate. The compounds with the highest concentrations on 28th day were 4-p-coumaroylquinonic acid, (-)-epicatechin, quercetin-3-O-rutinoside, quercetin–rhamnoside, kaempherol-3-rutinoside and quercetin-3-gucoside. Brain lipid peroxidation (MDA) decreased (p&lt;0.05) on the 21st and 28th day in the PSE group. Increase (p&lt;0.05) in GSH concentration was observed on the 21st and 28th and SOD activity on the 28th day. Catalase activity was unchanged. It could be hypothesized that highest PPH concentration-ratios, caused reduction of lipid peroxidation by radical scavenging and simultaneous induction of glutathione and SOD pathways.Conclusions: Screened compounds could be candidates for examining or creation of brain targeted “neuro-nutriceuticals” polyphenol mixtures

Association between aryl hydrocarbon receptor and 4-hydroxynonenal in oxidative stress-mediated chronic rhinosinusitis with nasal polyps

Objective: Сhronic rhinosinusitis with nasal polyps (CRSwNPs) is a distinct entity within the chronic rhinosinusitis group of diseases, which are chronic upper airway diseases with several pheno- and endotypes. Oxidative stress plays an important role in the pathogenesis of CRSwNPs. The aim was to assess the association between expression of the aryl hydrocarbon receptor (AhR) and 4-hydroxynonenal (4-HNE) in patients with CRSwNPs. Methods: The study included 26 patients who underwent endoscopic sinus surgery: 14 patients with CRSwNPs, and 12 controls with healthy sinus mucosa. Expression of AhR and 4-HNE was assessed in tissue samples using immunohistochemistry. The level of 4-HNE in serum samples was measured using the ELISA assay. Total oxidative capacity (TOC) was assessed by measuring the peroxidase activity. Results: Higher levels of 4-HNE expression were observed in tissues (3, range 1-3 vs. 0, range 0-0 p&lt;0.001) and serum (27.7±11.5 vs. 9.8±7.7 pmol/mg, p &lt; 0.001) samples of CRSwNPs patients, as compared to healthy controls. Higher expression of AhR was found in inflammatory cells (plasma cells, lymphocytes, eosinopholes) of CRSwNPs patients, compared to controls (3, range 1-3 vs. 2, range 1- 2, p = 0.001). There were no differences in TOC across groups (0.0285±0.0207 vs. 0.02978±0.0197 µM H2O2 eq., p=0.848). Patients with bronchial asthma (57%) had abundant eosinophiles in tissue samples. Patients with recalcitrant CRSwNPs had higher 4- HNE serum levels, compared to non-recalcitrant cases (27.3 vs. 24.2 pmol/mg, p=0.339). Conclusion: Patients suffering from CRSwNPs have oxidative stress mediated overexpression of AhR, which is linked to a chronic inflammatory response in the paranasal sinus tissues

Efficacy of Caffeic Acid on Diabetes and Its Complications in the Mouse

Diabetic dyslipidemia and hyperglycemia contribute to excessive reactive oxygen species (ROS) production, leading to deleterious complications, such as nephropathy, atherosclerosis and cardiac dysfunction, and target major organs in the body. The aim of this study was to investigate the effect of caffeic acid (CA) on mouse weight and survival, serum level of fasting blood glucose (FBG), serum lipid parameters and atherogenic indices, oxidative damage in blood, liver and kidney tissue, pathophysiological changes and their function markers in healthy and alloxan-induced type 1 diabetic mice. Diabetes was induced in mice with a single intravenous injection of alloxan (75 mg/kg). Two days later, CA (50 mg/kg) was given intraperitoneally for seven days in diabetic mice. Diabetes affected glucose level, lipid profile, hematological and biochemical parameters, induced DNA damage and apoptotic/necrotic death in whole blood cells, liver and kidney, leading to weight loss and a decreased lifespan. CA treatment of diabetic mice revealed a protective effect on the liver and kidney, hypoglycemic and hypolipidemic properties and high protection against atherogenic outcomes. The obtained results suggest that CA is a safe and potent agent against diabetes that acts as an effective antioxidant in reducing serum glucose, lipid profile and atherogenic indices, leading to increased lifespan in mice

Ružička days : International conference 16th Ružička Days “Today Science – Tomorrow Industry” : Proceedings

Proceedings contains articles presented at Conference divided into sections: open lecture (1), chemical analysis and synthesis (3), chemical and biochemical engineering (8), food technology and biotechnology (8), medical chemistry and pharmacy (3), environmental protection (11) and meeting of young chemists (2)

Animal model of psoriasis: anti inflammatory and antioxidant epigallocatechin gallate efficiency

Psorijaza je kronična upalna bolest, čije imunopatogeneza, prevencija i liječenje još uvijek nisu u cijelosti razriješene. Istražili smo moguće korisno djelovanje pripravka epigalokatehin galata (EGCG) na animalnom modelu psorijaze, prouzročene iritantom di-n-propil disulfidom (PPD), tijekom 5 dana. Antiupalno i antioksidativno djelovanje EGCG-a pratili smo kroz više različitih metoda kao što su određivanje broja stanica u trbušnoj šupljini, funkcionalna aktivnost makrofaga u peritonealnoj tekućini, patohistološka analiza promjena na koži pokusnih životinja, hematološke i biokemijske parametre krvi te metoda za procjenu antioksidativne učinkovitosti (reducirani glutation, lipidna peroksidacija). Rezultati ukazuju da pripravak EGCG-a smanjuje ukupan broj upalnih stanica u peritonealnoj tekućini miševa. Sve uočene promjene hematoloških i biokemijskih pokazatelja kretale su se unutar normalnih vrijednosti, a rezultati određivanja citokina u plazmi pokazali su da je PPD sam ili združen s EGCG-om izazvao veću razinu upalnih citokina, posebice, čimbenika stimulacije kolonija granulocita (G-CSF), zatim interleukina-6 (IL-6). Patohistološka analiza uzoraka kože pokazala je da združena topička primjena iritanta s testiranim tvarima u svim skupinama ima upalnu reakciju znatno slabijeg intenziteta od PPD-a. Genotoksični učinak istraživanih pripravaka procijenili smo pomoću komet testa i mikronukleus testa. Rezultati komet testa i mikronukleus testa pokazuju da EGCG nije genotoksičan za stanice periferne krvi miševa. Temeljem rezultata zaključujemo da istraživani pripravak EGCG-a, pokazuje protuupalni učinak, te da bi njegova primjena mogla poslužiti u sprječavanju i liječenju upalnih promjena psorijaze, bez toksičnih posljedica.Psoriasis is a chronic, immune-mediated disease, which immunopathogenesis, prevention and treatments are not completely resolved. We did some researches about positive effects of epigallocatehin gallate (EGCG) preparations on an animal model of psoriasis, in which the psoriasis was caused by di-n-propyl disulfide (PPD) irritant during 5 days. Anti-inflammatory and anti-oxidative activity of ECGC preparations were followed by various methods: valuation of cell numbers in abdominal cavity, functional activity of macrophages in peritoneal fluid, pathohistological analyses of transformations on experimental animals, hematological and biochemical parameters of blood and method of valuation of anti-oxidative efficiency (reduced glutathione, lipid peroxidation). The results indicate that EGCG preparations reduce number of inflammatory cells in peritoneal fluid of mice. All hematological and biochemical transformations indicate that it falls within the reference range and the results of cytokines' values in plasma indicate that PPD alone, or in aggregation with EGCG, caused a bigger level of anti-inflammatory cytokines, especially colony stimulating factore hormones (G-CSF) and interleukins-6 (IL-6). Pathohistological analysis of skin samples indicates that a topical use of the irritant with tested substances, in all groups, causes significantly lower inflammatory reaction than PPD itself. We estimated genotoxic effect of examined preparations with the comet test and the micronucleus test. The results of tests show no genotoxicity induced by ECGC preparations in peripheral blood cells of mice. We concluded that researched ECGC preparations showed anti-inflammatory effect. It could be used for suppression and treatment of inflammatory presents in psoriasis, without any toxical effects

Bioavailability, metabolism and antioxidative defence of polyphenols in different organs of C57BL/6 mice

U ovome radu je istražena in vivo farmakokinetika-apsorpcija, metabolizam i raspodjela polifenolnih spojeva iz vodenog ekstrakta cvijeta trnine (Prunus spinosa L.), te vodenog ekstrakta cvijeta trnine u kombinaciji s prehranom obogaćenom proteinima, mastima i ugljikohidratima u organima (tanko crijevo, jetra, mozak, bubreg) miša C57BL/6. Također je istražen bioakumulacijski potencijal nakupljanja molekula polifenola u navedenim organima, te biološki raspoložive maksimalne koncentracije pojedinih skupina molekula polifenola. Osim navedenog, istraženi su glavni biomarkeri oksidacijskog stresa (MDA, PC), aktivacija antioksidacijskih enzima (SOD, GSH, CAT) i antioksidacijski kapaciteti navedenih tkiva. Dobiveni rezultati pokazuju da se od ukupno 32 vrste polifenolnih spojeva koje nalazimo u ekstraktu cvijeta trnine, apsorpcijom i bioraspodjelom po organima u različitim vremenima bioakumuliraju različite vrste polifenolnih spojeva što potvrđuje postavljenu hipotezu o selektivnoj preferenciji za bioakumulacijom i zadržavanjem pojedinog tipa polifenolnih spojeva unutar različitih organa. Rezultati analize antioksidacijskog sustava su također pokazali koje pojedine skupine polifenola i njihovih metabolita aktiviraju enzime uključene u antioksidacijski sustav obrane (SOD, GSH, CAT), a izmjereni antioksidacijski kapaciteti tkiva ukazuju da pojedini organi u pokusnim skupinama pokazuju relativno visoki antioksidacijski kapacitet u odnosu na kontrolnu skupinu životinja. Dobiveni rezultati ovog istraživanja pridonose boljem razumijevanju afiniteta pojedinih skupina polifenola prema pojedinim tkivima i organima s temeljnim ciljem postizanja boljeg antioksidacijskog statusa organizma.This thesis deals with the in vivo pharmacokinetics-absorption, metabolism and distribution of polyphenolic compounds from the sloe (Prunus spinosa L.) flower water extract and the water extract of sloe in combination with a diet enriched by proteins, fat and carbohydrates in the organs (small intestine, liver, brain, kidney) of the mouse C57BL/6. Furthermore, the bioaccumulative potential of polyphenolic compounds in these organs was also investigated, as well as the bioavailable maximum concentration of polyphenolic molecules. In addition, major oxidative stress biomarkers (MDA, PC), antioxidant enzyme activation (SOD, GSH, CAT), and antioxidative capacity of selected tissues were investigated. The obtained results show that out of a total of 32 types of polyphenolic compounds found in the sloe flower extract, various types of polyphenolic compounds bioaccumulate by absorption and biodistribution within organs at different times which confirms the set hypothesis of selective preference for bioaccumulation and retention of a certain type of polyphenolic compounds within different organs. The results of the antioxidation system analysis have also shown which groups of polyphenols and their metabolites activate specific enzymes involved in the antioxidative defence system (SOD, GSH, CAT) and the measured antioxidant tissue capacity indicates that certain organs in the test groups exhibit a relatively high antioxidative capacity in relation to the animal control group. The results of this study contribute to a better understanding of the affinity of certain groups of polyphenols to certain tissues and organs with the fundamental aim of achieving a better antioxidative status of the organism

Bioavailability, metabolism and antioxidative defence of polyphenols in different organs of C57BL/6 mice

U ovome radu je istražena in vivo farmakokinetika-apsorpcija, metabolizam i raspodjela polifenolnih spojeva iz vodenog ekstrakta cvijeta trnine (Prunus spinosa L.), te vodenog ekstrakta cvijeta trnine u kombinaciji s prehranom obogaćenom proteinima, mastima i ugljikohidratima u organima (tanko crijevo, jetra, mozak, bubreg) miša C57BL/6. Također je istražen bioakumulacijski potencijal nakupljanja molekula polifenola u navedenim organima, te biološki raspoložive maksimalne koncentracije pojedinih skupina molekula polifenola. Osim navedenog, istraženi su glavni biomarkeri oksidacijskog stresa (MDA, PC), aktivacija antioksidacijskih enzima (SOD, GSH, CAT) i antioksidacijski kapaciteti navedenih tkiva. Dobiveni rezultati pokazuju da se od ukupno 32 vrste polifenolnih spojeva koje nalazimo u ekstraktu cvijeta trnine, apsorpcijom i bioraspodjelom po organima u različitim vremenima bioakumuliraju različite vrste polifenolnih spojeva što potvrđuje postavljenu hipotezu o selektivnoj preferenciji za bioakumulacijom i zadržavanjem pojedinog tipa polifenolnih spojeva unutar različitih organa. Rezultati analize antioksidacijskog sustava su također pokazali koje pojedine skupine polifenola i njihovih metabolita aktiviraju enzime uključene u antioksidacijski sustav obrane (SOD, GSH, CAT), a izmjereni antioksidacijski kapaciteti tkiva ukazuju da pojedini organi u pokusnim skupinama pokazuju relativno visoki antioksidacijski kapacitet u odnosu na kontrolnu skupinu životinja. Dobiveni rezultati ovog istraživanja pridonose boljem razumijevanju afiniteta pojedinih skupina polifenola prema pojedinim tkivima i organima s temeljnim ciljem postizanja boljeg antioksidacijskog statusa organizma.This thesis deals with the in vivo pharmacokinetics-absorption, metabolism and distribution of polyphenolic compounds from the sloe (Prunus spinosa L.) flower water extract and the water extract of sloe in combination with a diet enriched by proteins, fat and carbohydrates in the organs (small intestine, liver, brain, kidney) of the mouse C57BL/6. Furthermore, the bioaccumulative potential of polyphenolic compounds in these organs was also investigated, as well as the bioavailable maximum concentration of polyphenolic molecules. In addition, major oxidative stress biomarkers (MDA, PC), antioxidant enzyme activation (SOD, GSH, CAT), and antioxidative capacity of selected tissues were investigated. The obtained results show that out of a total of 32 types of polyphenolic compounds found in the sloe flower extract, various types of polyphenolic compounds bioaccumulate by absorption and biodistribution within organs at different times which confirms the set hypothesis of selective preference for bioaccumulation and retention of a certain type of polyphenolic compounds within different organs. The results of the antioxidation system analysis have also shown which groups of polyphenols and their metabolites activate specific enzymes involved in the antioxidative defence system (SOD, GSH, CAT) and the measured antioxidant tissue capacity indicates that certain organs in the test groups exhibit a relatively high antioxidative capacity in relation to the animal control group. The results of this study contribute to a better understanding of the affinity of certain groups of polyphenols to certain tissues and organs with the fundamental aim of achieving a better antioxidative status of the organism