6 research outputs found
Correlaci贸n entre recuento de micron煤cleos y h谩bito tab谩quico: Un estudio hospitalario.
Background: Micronucleus is a microscopically visible cyto-plasmic chromatin mass in the extranuclear vicinity, originating from aberrant mitosis, which consists of eccentric chromosomes that have failed to reach spindle poles during mitosis. The present study was designed to evaluate and compare cytogenetic changes in the buccal mucosa of smokers and non-smokers based on the occurrence of micronuclei. The study aimed to determine the correlation between the micronuclei count and the frequency and duration of smoking habit. Materials and Methods: Two groups (smokers and non-smokers) of 34 individuals each were examined. Cytological buccal smears were taken from participants using a moistened wooden spatula and stained with standard Papanicolaou stain. Presence of micronuclei was assessed at 40X magnification using a light microscope and a count per 500 cells was determined. The results of the study were analyzed statistically using Mann-Whitney U test, Spearman's rank correlation coefficient and Student t-test. Result: Smears from smokers showed a significant increase in the total number of micronuclei per 500 cell count compared to non-smokers. There was a strong positive correlation between the occurrence of micronuclei and the frequency and duration of smoking. A age-related increase in older age groups was also observed. Conclusion: The study reveals a strong positive correlation between the occurrence of micronuclei and the frequency and duration of smoking. This observation is vital in the utilization of the micronuclei detection in smears as a prognostic, educational and interventional tool in the management of patients with smoking habits.Antecedentes: El micron煤cleo es una masa de cromatina citoplasm谩tica microsc贸picamente visible en el 谩rea extranuclear, que se origina a partir de la mitosis aberrante, y que consiste en cromosomas exc茅ntricos que no han podido alcanzar los polos del huso durante la mitosis. El presente estudio fue dise帽ado para evaluar y comparar los cambios citogen茅ticos en la mucosa bucal de fumadores y no fumadores en funci贸n de la aparici贸n de micron煤cleos. El estudio tuvo como objetivo determinar la correlaci贸n entre el recuento de micron煤cleos y la frecuencia y duraci贸n del h谩bito de fumar. Materiales and M茅todos: Se examinaron dos grupos (fumadores y no fumadores) de 34 individuos cada uno. Se tomaron frotis bucales citol贸gicos de todos los participantes con una esp谩tula de madera humedecida y se ti帽eron con la tinci贸n est谩ndar de Papanicolaou. La presencia de micron煤cleos se evalu贸 al microscopio 贸ptico con un aumento de 40X y se determin贸 un recuento por 500 c茅lulas. Los resultados del estudio se analizaron estad铆sticamente utilizando la prueba U de Mann-Whitney, el coeficiente de correlaci贸n de rango de Spearman y la prueba t de Student. Resultados: Los frotis de fumadores mostraron un aumento significativo en el n煤mero total de micron煤cleos por 500 c茅lulas en comparaci贸n con los no fumadores. Hubo una fuerte correlaci贸n positiva entre la aparici贸n de micron煤cleos y la frecuencia y duraci贸n del tabaquismo. Tambi茅n se observ贸 un aumento relacionado con la edad en los grupos de mayor edad. Conclusi贸n: el estudio revela una fuerte correlaci贸n positiva entre la aparici贸n de micron煤cleos y la frecuencia y duraci贸n del tabaquismo. Esta observaci贸n es vital en la utilizaci贸n de la detecci贸n de micron煤cleos en frotis como una herramienta pronostica, educativa e intervencionista en el manejo de pacientes con h谩bitos de fumar
The correlation between micronuclei count and smoking behavior: A hospital-based study.
Background: Micronucleus is a microscopically visible cyto-plasmic chromatin mass in the extranuclear vicinity, originating from aberrant mitosis, which consists of eccentric chromosomes that have failed to reach spindle poles during mitosis. The present study was designed to evaluate and compare cytogenetic changes in the buccal mucosa of smokers and non-smokers based on the occurrence of micronuclei. The study aimed to determine the correlation between the micronuclei count and the frequency and duration of smoking habit. Materials and Methods: Two groups (smokers and non-smokers) of 34 individuals each were examined. Cytological buccal smears were taken from participants using a moistened wooden spatula and stained with standard Papanicolaou stain. Presence of micronuclei was assessed at 40X magnification using a light microscope and a count per 500 cells was determined. The results of the study were analyzed statistically using Mann-Whitney U test, Spearman's rank correlation coefficient and Student t-test. Result: Smears from smokers showed a significant increase in the total number of micronuclei per 500 cell count compared to non-smokers. There was a strong positive correlation between the occurrence of micronuclei and the frequency and duration of smoking. A age-related increase in older age groups was also observed. Conclusion: The study reveals a strong positive correlation between the occurrence of micronuclei and the frequency and duration of smoking. This observation is vital in the utilization of the micronuclei detection in smears as a prognostic, educational and interventional tool in the management of patients with smoking habits.Antecedentes: El micron煤cleo es una masa de cromatina citoplasm谩tica microsc贸picamente visible en el 谩rea extranuclear, que se origina a partir de la mitosis aberrante, y que consiste en cromosomas exc茅ntricos que no han podido alcanzar los polos del huso durante la mitosis. El presente estudio fue dise帽ado para evaluar y comparar los cambios citogen茅ticos en la mucosa bucal de fumadores y no fumadores en funci贸n de la aparici贸n de micron煤cleos. El estudio tuvo como objetivo determinar la correlaci贸n entre el recuento de micron煤cleos y la frecuencia y duraci贸n del h谩bito de fumar. Materiales and M茅todos: Se examinaron dos grupos (fumadores y no fumadores) de 34 individuos cada uno. Se tomaron frotis bucales citol贸gicos de todos los participantes con una esp谩tula de madera humedecida y se ti帽eron con la tinci贸n est谩ndar de Papanicolaou. La presencia de micron煤cleos se evalu贸 al microscopio 贸ptico con un aumento de 40X y se determin贸 un recuento por 500 c茅lulas. Los resultados del estudio se analizaron estad铆sticamente utilizando la prueba U de Mann-Whitney, el coeficiente de correlaci贸n de rango de Spearman y la prueba t de Student. Resultados: Los frotis de fumadores mostraron un aumento significativo en el n煤mero total de micron煤cleos por 500 c茅lulas en comparaci贸n con los no fumadores. Hubo una fuerte correlaci贸n positiva entre la aparici贸n de micron煤cleos y la frecuencia y duraci贸n del tabaquismo. Tambi茅n se observ贸 un aumento relacionado con la edad en los grupos de mayor edad. Conclusi贸n: el estudio revela una fuerte correlaci贸n positiva entre la aparici贸n de micron煤cleos y la frecuencia y duraci贸n del tabaquismo. Esta observaci贸n es vital en la utilizaci贸n de la detecci贸n de micron煤cleos en frotis como una herramienta pronostica, educativa e intervencionista en el manejo de pacientes con h谩bitos de fumar
Radiographic assessment of distribution of mandibular third molar impaction: A retrospective study
Introduction: Third molars are the most common teeth that may follow an abortive eruption path and become impacted as a result of pathology, anatomical structures or insufficient osseous space posterior to the second molars. Aims and Objectives: The present study evaluated (1) the distribution of the impaction of mandibular third molar; (2) the distribution of the patterns of impaction radiographically; and (3) the gender distribution for pattern of impaction. Materials and Methods: This hospital-based retrospective study was conducted over a course of 6 months in the Department of Oral Medicine and Radiology and presents the analysis of 122 panoramic radiographs of patients between the age group of 18-30 years. They were interpreted and assessed for the impaction of mandibular third molars. Statistical analysis was done by Chi-square test. Results: Bilateral impaction of mandibular third molar is more common than unilateral in both the sexes, with mesioangular being the most common pattern. In males, mesioangular pattern was followed by horizontal, whereas in females it was followed by vertical. Conclusion: The present study provides useful data regarding the radiographic status of impacted mandibular third molars in patients