Contribution of haematological factors to the distributions and estimation of Eustrongyliides africanus larvae densities in Clarias gariepinus and Clarias anguillaris from Bida floodplain of Nigeria

The contributions of hematological factors to the distribution and estimations of Eustrongylides africanus larvae densities in Clarias gariepinus and C. anguillaris of Bida floodplain of Nigeria were documented for the first time. The hematological factors making the most important contributions to the distributions of E. africanus larvae infections in clarias species are mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH), mean corpuscular volume (MCV) and neutrophils count, in descending order of magnitude; having the manifestations for the months of January, March, September, and December of the year being closely related. Five haematological factors (neutrophils, lymphocytes and eosinophils counts; MCH and MCV) having positive or negative correlation coefficient (r) between 0.50 and 0.85 contributed to the estimated of E.africanus larvae densities in the wild population of Clarias specie

Unconventional Substance Use among Young People in Urban Lagos and the Implications for Sexual and Reproductive Health

Previous studies have examined conventional substance use among young people but little is known about unconventional substance use in this population. Hence, this study sought to explore unconventional substance use among young people in urban Lagos and the implications for sexual and reproductive health (SRH). The study employed cluster sampling strategy in administering 1200 copies of a structured questionnaire among in-school and out-of-school young people (10-24 years old) across selected areas in urban Lagos. Results indicated that the social environment of young people influenced their involvement in unconventional substance use (p<0.05) in the study setting. Older young people, those out-of-school, exposure to unconventional substance use through peers, through television and those less religious were more likely to report ever use of any unconventional substance. Tramadol, cough syrup, and rohypnol were the most popular unconventional substances ever used among both male and female respondents. Also, while young females who used various unconventional substances were vulnerable to STIs owing to poor preventive behaviour, multiple sexual partnership was rampant among their male counterparts though rich preventive culture prevailed among them. Thus, unconventional drug use among young people has a strong probability of increasing health burden through risky SRH in the study setting

Isolation, characterization and immunochemical studies on fibrous proteins from cowry shell (Cypraea moneta, Linnaeus)

Background: Biomaterials are non-drug substances used to treat, enhance or replace functions of body tissues or organs. Natural sources of biomaterials have recently become the focus of several research activities. Cowry shell constitutes one of the most promising natural sources of biomaterials because of its chemical stability, biodegradability and biocompatibility in the body. However, its applications may be limited due to immunogenic and toxic responses that may occur following implantation, hence this study.Materials and Methods: Crude fibrous protein extracted with citrate buffer from pulverised cowry shells (Cypraea moneta (L)), was resolved into two components (CSP1 and CSP2) by gel filtration. Immunological studies were performed with antisera obtained from rabbits by double immunodiffusion and immunoelectrophoresis techniques. Mice treated with the proteins were observed for signs of toxicity and their liver, kidney, lungs and spleen were processed histologically.Results: The native molecular weight of CSP1 and CSP2 determined by gel filtration were 91kDa and 33kDa respectively. CSP1 and CSP2 displayed single bands on SDS-PAGE with subunit molecular weight values of 19kDa and 19.5kDa respectively. Antisera obtained from rabbits immunised with the crude citrate buffer extracts precipitated the antigen in double immunodiffusion tests. Histopathological examinations revealed a dose-dependent damaging effect of the shell proteins on liver, kidney, lung and spleen tissues of the treated mice.Conclusion: This study showed that cowry shells contain fibrous proteins which are immunogenic and toxic in mice at relatively high concentrations, causing visible organ damage without concurrent physical manifestations.Keywords: cowry shell protein, purification, immune activation, toxicit

Sustainability of multifaceted usage of biomass: A review

The paper focuses on collection of information on recent multifaceted usage of biomass materials with critical examination on its sustainability. The use of biomass is becoming popular, with wide global acceptance as it is considered as green technology. The use of biomass products across industrial parallels, the material combination and production processes were elucidated in this paper. Biomass materials are seen as affordable alternative to conventional materials for domestic and industrial applications. The multifaceted use of biomass, which includes, energy generation, metallurgical applications, construction purposes, reinforcement in metal matrix composite, microelectromechanical system, biochemical and traditional medicine were discussed. This underscores the need to develop a sustainable plan to meet with its diverse usage to be beyond laboratory efforts. This paper examined whether the availability of biomass can sustain its multifaceted usage or not. It also examined the modalities to ensure sustainable use of biomass. Different policies were highlighted and discussed in line with continuous multifaceted use of biomas

Global age-sex-specific mortality, life expectancy, and population estimates in 204 countries and territories and 811 subnational locations, 1950–2021, and the impact of the COVID-19 pandemic: a comprehensive demographic analysis for the Global Burden of Disease Study 2021

Background: Estimates of demographic metrics are crucial to assess levels and trends of population health outcomes. The profound impact of the COVID-19 pandemic on populations worldwide has underscored the need for timely estimates to understand this unprecedented event within the context of long-term population health trends. The Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2021 provides new demographic estimates for 204 countries and territories and 811 additional subnational locations from 1950 to 2021, with a particular emphasis on changes in mortality and life expectancy that occurred during the 2020–21 COVID-19 pandemic period. Methods: 22 223 data sources from vital registration, sample registration, surveys, censuses, and other sources were used to estimate mortality, with a subset of these sources used exclusively to estimate excess mortality due to the COVID-19 pandemic. 2026 data sources were used for population estimation. Additional sources were used to estimate migration; the effects of the HIV epidemic; and demographic discontinuities due to conflicts, famines, natural disasters, and pandemics, which are used as inputs for estimating mortality and population. Spatiotemporal Gaussian process regression (ST-GPR) was used to generate under-5 mortality rates, which synthesised 30 763 location-years of vital registration and sample registration data, 1365 surveys and censuses, and 80 other sources. ST-GPR was also used to estimate adult mortality (between ages 15 and 59 years) based on information from 31 642 location-years of vital registration and sample registration data, 355 surveys and censuses, and 24 other sources. Estimates of child and adult mortality rates were then used to generate life tables with a relational model life table system. For countries with large HIV epidemics, life tables were adjusted using independent estimates of HIV-specific mortality generated via an epidemiological analysis of HIV prevalence surveys, antenatal clinic serosurveillance, and other data sources. Excess mortality due to the COVID-19 pandemic in 2020 and 2021 was determined by subtracting observed all-cause mortality (adjusted for late registration and mortality anomalies) from the mortality expected in the absence of the pandemic. Expected mortality was calculated based on historical trends using an ensemble of models. In location-years where all-cause mortality data were unavailable, we estimated excess mortality rates using a regression model with covariates pertaining to the pandemic. Population size was computed using a Bayesian hierarchical cohort component model. Life expectancy was calculated using age-specific mortality rates and standard demographic methods. Uncertainty intervals (UIs) were calculated for every metric using the 25th and 975th ordered values from a 1000-draw posterior distribution. Findings: Global all-cause mortality followed two distinct patterns over the study period: age-standardised mortality rates declined between 1950 and 2019 (a 62·8% [95% UI 60·5–65·1] decline), and increased during the COVID-19 pandemic period (2020–21; 5·1% [0·9–9·6] increase). In contrast with the overall reverse in mortality trends during the pandemic period, child mortality continued to decline, with 4·66 million (3·98–5·50) global deaths in children younger than 5 years in 2021 compared with 5·21 million (4·50–6·01) in 2019. An estimated 131 million (126–137) people died globally from all causes in 2020 and 2021 combined, of which 15·9 million (14·7–17·2) were due to the COVID-19 pandemic (measured by excess mortality, which includes deaths directly due to SARS-CoV-2 infection and those indirectly due to other social, economic, or behavioural changes associated with the pandemic). Excess mortality rates exceeded 150 deaths per 100 000 population during at least one year of the pandemic in 80 countries and territories, whereas 20 nations had a negative excess mortality rate in 2020 or 2021, indicating that all-cause mortality in these countries was lower during the pandemic than expected based on historical trends. Between 1950 and 2021, global life expectancy at birth increased by 22·7 years (20·8–24·8), from 49·0 years (46·7–51·3) to 71·7 years (70·9–72·5). Global life expectancy at birth declined by 1·6 years (1·0–2·2) between 2019 and 2021, reversing historical trends. An increase in life expectancy was only observed in 32 (15·7%) of 204 countries and territories between 2019 and 2021. The global population reached 7·89 billion (7·67–8·13) people in 2021, by which time 56 of 204 countries and territories had peaked and subsequently populations have declined. The largest proportion of population growth between 2020 and 2021 was in sub-Saharan Africa (39·5% [28·4–52·7]) and south Asia (26·3% [9·0–44·7]). From 2000 to 2021, the ratio of the population aged 65 years and older to the population aged younger than 15 years increased in 188 (92·2%) of 204 nations. Interpretation: Global adult mortality rates markedly increased during the COVID-19 pandemic in 2020 and 2021, reversing past decreasing trends, while child mortality rates continued to decline, albeit more slowly than in earlier years. Although COVID-19 had a substantial impact on many demographic indicators during the first 2 years of the pandemic, overall global health progress over the 72 years evaluated has been profound, with considerable improvements in mortality and life expectancy. Additionally, we observed a deceleration of global population growth since 2017, despite steady or increasing growth in lower-income countries, combined with a continued global shift of population age structures towards older ages. These demographic changes will likely present future challenges to health systems, economies, and societies. The comprehensive demographic estimates reported here will enable researchers, policy makers, health practitioners, and other key stakeholders to better understand and address the profound changes that have occurred in the global health landscape following the first 2 years of the COVID-19 pandemic, and longer-term trends beyond the pandemic. Funding: Bill & Melinda Gates Foundation

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Antimalarial and toxicological effects of aqueous leaf extract of Lawsonia inermis Lythraceae (Linn) alone and in combination with Alstonia boonei on Plasmodium berghei- infected mice

Background: Lawsonia inermis Lythraceae (Linn) and Alstonia boonei De wild are well known for their antimalarial potentials in traditional medicine. However, there is no scientific evidence for this claim.Objective: The aim was to evaluate the in vivo antimalarial and toxicological effects of the aqueous extracts of Lawsonia inermis alone and in combination with Alstonia boonei in Plasmodium berghei infected miceMethod: Fifty four mice were divided into 9 groups of 6 mice each. Groups 1 – 3 were healthy control, P. berghei infected and P. berghei infected mice treated with Chloroquine dose (positive control), respectively. Groups 4 – 6 were P. berghei infected mice orally administered 50, 100, and 200 mg/kg of Lawsonia inermis while groups 7 – 9 were also P. berghei infected mice but received 50, 100, and 200 mg/kg of Lawsonia inermis and Alstonia boonei extracts. All infected mice were inoculated with malaria parasites on the first day and extracts administrationcommenced on the fourth day and lasted for 7 days. The mice were sacrificed on the 14th day post-inoculation and the activities of liver, kidney and serum enzymes were analyzed.Results: The treatment of parasitized mice with Lawsonia inermis at 100 and 200 mg/kg body weight showed the most significant (p&lt;0.05) antimalarial activity against P. berghei infection. A combined treatment with Lawsonia inermis and Alstonia boonei suppressed malarial infection significantly (p&lt;0.05) at 50 mg/kg and increased packed cell volume (PCV). Administration of 100 mg/kg L. inermis significantly (p&lt;0.05) elevated the activities of alanine amino transaminase (ALT) and aspartate amino transaminase (AST) in the liver and kidneys but not in theserum. Also, alkaline phosphatase (ALP) activity was increased in the liver, kidneys and serum by 100 mg/kg Lawsonia inermis alone.Conclusion: Findings revealed the chemosuppressive potential of Lawsonia inermis against P. berghei as well as its toxicity to the liver and kidney.Keywords:  Lawsonia inermis, Alstonia boonei, Plasmodium berghei, Parasitemia, Amino transferase

Obesity prevalence in adult residents of ile-ife, nigeria

Background and Purpose: Few data on obesity exist on Sub-Sahara population in Africa. This study investigated the prevalence of obesity and Body Mass Index (BMI) percentile and quartiles in accordance with sex and age in adult residents of the historic ancient semi-urban communityof Ile-Ife, South-West, Nigeria.Materials and Methods: 2097 adults aged 21 years and above were recruited into the door-to-door survey through a multi-stage cluster sampling technique. The World Health Organization (WHO) criteria based on BMI was used in the definition of overweight and obesity. Height and weight were measured using standardized procedures.Results: The mean age and BMI of the participants were 44.2 years and 24.2 Kg/m2 respectively. Although agematched; the females had higher BMI values compared to males (23.8 vs. 24.5 Kg/m2). The overall crude prevalence of overweight (25.0-29.9 Kg/m2) and obesity (>30 Kg/m2) were 20.3% and 12.5% respectively. The rates of overweight (17.9 vs. 22.1 Kg/m2) and obesity (9.7 vs. 14.5 Kg/m2) were both higher in women than men. Obesity increased across age gradient from young to old adults; peaking in the 60-69- year age group. The first to fourth BMI quartiles were = 20.4 Kg/m2, 20.5-24.1 Kg/m2, 24.2-25.2 Kg/m2, = 25.3 Kg/m2respectively in the study population. At all ages; more females (32.4%) than males (24.7%) were placed within fourth BMI quartile. The 95th percentile BMI in the study population was 33.4Kg/m2.Conclusion: Overweight and obesity are common in Nigerians, particular among females and elderly. The prevalence estimates of overweight and obesity in Nigerians is comparable with prevalence among Blacks in otherpopulations. Keywords: Overweight, Obesity, Prevalence, Pattern, Sub Saharan Africa, Ile-Ife, Nigeria