1,069 research outputs found
Analytical Validation and Capabilities of the Epic CTC Platform: Enrichment-Free Circulating Tumour Cell Detection and Characterization
The Epic Platform was developed for the unbiased detection and molecular characterization of circulating tumour cells (CTCs). Here, we report assay performance data, including accuracy, linearity, specificity and intra/inter-assay precision of CTC enumeration in healthy donor (HD) blood samples spiked with varying concentrations of cancer cell line controls (CLCs). Additionally, we demonstrate clinical feasibility for CTC detection in a small cohort of metastatic castrate-resistant prostate cancer (mCRPC) patients. The Epic Platform demonstrated accuracy, linearity and sensitivity for the enumeration of all CLC concentrations tested. Furthermore, we established the precision between multiple operators and slide staining batches and assay specificity showing zero CTCs detected in 18 healthy donor samples. In a clinical feasibility study, at least one traditional CTC/mL (CK+, CD45-, and intact nuclei) was detected in 89 % of 44 mCRPC samples, whereas 100 % of samples had CTCs enumerated if additional CTC subpopulations (CK-/CD45- and CK+ apoptotic CTCs) were included in the analysis. In addition to presenting Epic Platform’s performance with respect to CTC enumeration, we provide examples of its integrated downstream capabilities, including protein biomarker expression and downstream genomic analyses at single cell resolution
Precision Measurement of the Radiative Decay of the Free Neutron
The standard model predicts that, in addition to a proton, an electron, and
an antineutrino, a continuous spectrum of photons is emitted in the
decay of the free neutron. We report on the RDK II experiment which measured
the photon spectrum using two different detector arrays. An annular array of
bismuth germanium oxide scintillators detected photons from 14 to 782~keV. The
spectral shape was consistent with theory, and we determined a branching ratio
of 0.00335 0.00005 [stat] 0.00015 [syst]. A second detector array
of large area avalanche photodiodes directly detected photons from 0.4 to
14~keV. For this array, the spectral shape was consistent with theory, and the
branching ratio was determined to be 0.00582 0.00023 [stat] 0.00062
[syst]. We report the first precision test of the shape of the photon energy
spectrum from neutron radiative decay and a substantially improved
determination of the branching ratio over a broad range of photon energies
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An overview of ISCAT 2000
The Investigation of Sulfur Chemistry in the Antarctic Troposphere (ISCAT) took place over the timer period of 15 November to 31 December in the year 2000. The study location was the Amundsen Scott Station in Antarctica. ISCAT 2000 defines the second phase of a program designed to explore tropospheric chemistry in Antarctica. As in 1998, the 2000 ISCAT study revealed a strong oxidizing environment at South Pole (SP). During the 2000 investigation, however, the suite of measurements was greatly expanded. These new measurements established the recycling of reactive nitrogen as a critical component of this unique environment. This paper first presents the historical background leading up to the ISCAT 2000 observations; then it focuses on providing a summary of the year 2000 results and contrasts these with those recorded during 1998. Important developments made during the 2000 study included the recording of SP data for several species being emitted from the snowpack. These included NO, H 2O2 and CH2O. In this context, eddy-diffusion flux measurements provided the first quantitative estimates of the SP NO and NOx snow-to-atmosphere fluxes. This study also revealed that HNO 3 and HO2NO2 were major sink species for HOx and NOx radicals. And, it identified the critical factors responsible for SP NO levels exceeding those at other polar sites by nearly an order of magnitude. Finally, it reports on the levels of gas phase sulfur species and provides evidence indicating that the absence of DMS at SP is most likely due to its greatly shorten chemical lifetime in the near vicinity of the plateau. It is proposed that this is due to the influence of NO on the distribution of OH in the lower free troposphere over a region that extends well beyond the plateau itself. Details related to each of the above findings plus others can be found in the 11 accompanying Special Issue papers. © 2004 Elsevier Ltd. All rights reserved
Magnetic Reversal on Vicinal Surfaces
We present a theoretical study of in-plane magnetization reversal for vicinal
ultrathin films using a one-dimensional micromagnetic model with
nearest-neighbor exchange, four-fold anisotropy at all sites, and two-fold
anisotropy at step edges. A detailed "phase diagram" is presented that catalogs
the possible shapes of hysteresis loops and reversal mechanisms as a function
of step anisotropy strength and vicinal terrace length. The steps generically
nucleate magnetization reversal and pin the motion of domain walls. No sharp
transition separates the cases of reversal by coherent rotation and reversal by
depinning of a ninety degree domain wall from the steps. Comparison to
experiment is made when appropriate.Comment: 12 pages, 8 figure
Robust modeling of human contact networks across different scales and proximity-sensing techniques
The problem of mapping human close-range proximity networks has been tackled
using a variety of technical approaches. Wearable electronic devices, in
particular, have proven to be particularly successful in a variety of settings
relevant for research in social science, complex networks and infectious
diseases dynamics. Each device and technology used for proximity sensing (e.g.,
RFIDs, Bluetooth, low-power radio or infrared communication, etc.) comes with
specific biases on the close-range relations it records. Hence it is important
to assess which statistical features of the empirical proximity networks are
robust across different measurement techniques, and which modeling frameworks
generalize well across empirical data. Here we compare time-resolved proximity
networks recorded in different experimental settings and show that some
important statistical features are robust across all settings considered. The
observed universality calls for a simplified modeling approach. We show that
one such simple model is indeed able to reproduce the main statistical
distributions characterizing the empirical temporal networks
Why Primate Models Matter
Research involving nonhuman primates (NHPs) has played a vital role in many of the medical and scientific advances of the past century. NHPs are used because of their similarity to humans in physiology, neuroanatomy, reproduction, development, cognition, and social complexity – yet it is these very similarities that make the use of NHPs in biomedical research a considered decision. As primate researchers, we feel an obligation and responsibility to present the facts concerning why primates are used in various areas of biomedical research. Recent decisions in the United States, including the phasing out of chimpanzees in research by the National Institutes of Health and the pending closure of the New England Primate Research Center, illustrate to us the critical importance of conveying why continued research with primates is needed. Here we review key areas in biomedicine where primate models have been, and continue to be, essential for advancing fundamental knowledge in biomedical and biological research
Inmate Recidivism as a Measure of Private Prison Performance
The growth of the private corrections industry has elicited interest in the comparative performance of state and private prisons. One way to measure the service quality of private prisons is to examine inmates' postrelease performance. Current empirical evidence is limited to four studies, all conducted in Florida. This analysis replicates and adds to the Florida measures in a different state and enhances previous methods. It uses data for a large cohort of Oklahoma state prison inmates released between 1997 and 2001. Controlling for known covariates, multivariate survival analysis revealed comparative rates of reincarceration for inmates in multiple exposure and comparison groups. These results are unique among prior studies on this topic; private prison inmates had a greater hazard of recidivism in all eight models tested, six of which were statistically significant. Finding no empirical support for claims of superior service from private corrections, the authors discuss policy implications and prospects for future research.Yeshttps://us.sagepub.com/en-us/nam/manuscript-submission-guideline
Birthweight and risk markers for type 2 diabetes and cardiovascular disease in childhood: the Child Heart and Health Study in England (CHASE).
AIMS/HYPOTHESIS: Lower birthweight (a marker of fetal undernutrition) is associated with higher risks of type 2 diabetes and cardiovascular disease (CVD) and could explain ethnic differences in these diseases. We examined associations between birthweight and risk markers for diabetes and CVD in UK-resident white European, South Asian and black African-Caribbean children.
METHODS: In a cross-sectional study of risk markers for diabetes and CVD in 9- to 10-year-old children of different ethnic origins, birthweight was obtained from health records and/or parental recall. Associations between birthweight and risk markers were estimated using multilevel linear regression to account for clustering in children from the same school.
RESULTS: Key data were available for 3,744 (66%) singleton study participants. In analyses adjusted for age, sex and ethnicity, birthweight was inversely associated with serum urate and positively associated with systolic BP. After additional height adjustment, lower birthweight (per 100 g) was associated with higher serum urate (0.52%; 95% CI 0.38, 0.66), fasting serum insulin (0.41%; 95% CI 0.08, 0.74), HbA1c (0.04%; 95% CI 0.00, 0.08), plasma glucose (0.06%; 95% CI 0.02, 0.10) and serum triacylglycerol (0.30%; 95% CI 0.09, 0.51) but not with BP or blood cholesterol. Birthweight was lower among children of South Asian (231 g lower; 95% CI 183, 280) and black African-Caribbean origin (81 g lower; 95% CI 30, 132). However, adjustment for birthweight had no effect on ethnic differences in risk markers.
CONCLUSIONS/INTERPRETATION: Birthweight was inversely associated with urate and with insulin and glycaemia after adjustment for current height. Lower birthweight does not appear to explain emerging ethnic difference in risk markers for diabetes
A Simple Model for Anisotropic Step Growth
We consider a simple model for the growth of isolated steps on a vicinal
crystal surface. It incorporates diffusion and drift of adatoms on the terrace,
and strong step and kink edge barriers. Using a combination of analytic methods
and Monte Carlo simulations, we study the morphology of growing steps in
detail. In particular, under typical Molecular Beam Epitaxy conditions the step
morphology is linearly unstable in the model and develops fingers separated by
deep cracks. The vertical roughness of the step grows linearly in time, while
horizontally the fingers coarsen proportional to . We develop scaling
arguments to study the saturation of the ledge morphology for a finite width
and length of the terrace.Comment: 20 pages, 12 figures; [email protected]
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