The effect of spray‐dried porcine plasma on gilthead seabream (Sparus aurata) intestinal microbiota

The effect of spray‐dried porcine plasma (SDPP) on the intestinal histological organization and autochthonous microbiota composition was evaluated in Sparus aurata. Fish were fed a basal diet (51 g/kg protein, 17 g/kg fat, 20.6 MJ/kg gross energy) and a diet containing 3 g/kg SDPP for 95 days (initial body weight, BW = 9.5 ± 0.2g, mean ± SD). The inclusion of SDPP promoted growth (p .05) between both groups. Intestinal microbiota was dominated by Proteobacteria (>85%) and Firmicutes (5%–12%), whereas Bacteroidetes never represented more than 1.5%. γ‐Proteobacteria, and Bacilli and Clostridia were the predominant classes. The short administration of SDPP (20 days) resulted in changes in microbiota diversity and richness associated with an increase in the sequences of the genus Lactobacillus and to a decrease in the genus Vibrio, whereas these changes were reverted at 95 days. Intestinal goblet cell density was not correlated to microbiota diversity and richness changes rather than to the immunostimulatory effect of the SDPP.info:eu-repo/semantics/acceptedVersio

Biofilm inhibition of pathogenic strains by extracellular products (ECPS) of Shewanella.sp Probiotic

Shewanella putrefaciens Pdp11 and V.proteolyticus DCF 12.2 are strains isolated by our research group. Recent works have been focused in the identification of substances as alternative of anti-biofilm methods and their implication in surface attachment inhibition. In this research, S. putrefaciens Pdp11 and V.proteolyticus DCF 12.2 have been cultured under different growth conditions (temperature, culture media and during 24 and 48 hours of incubation) and their extracellular products (ECPs) have been extracted and tested as potential postbiotics that affect the biofilm formation of several fish pathogenic strains. This assay results evidence that Pdp11_Pmix_2324 ECPs have showed the most impact in the biofilm formation of pathogenic strains. Therefore, ECPs secreted by Pdp11 and V.proteolyticus DCF 12.2 are implicated in the inhibition to adhesion of pathogens on surfaces.Proyecto de investigación PID2020-113637RB-C22. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech