Oficinas de Biotecnologia para o Ensino Médio: Antioxidantes, a Fonte da juventude?

Theory and practice about biotechnology-related issues have been topics of workshops for students and teachers of the Post Graduate in Biotechnology UNIVATES to secondary education students. We held ten different workshops aimed at the food production and health, among which stands out in this study, the workshop entitled "Antioxidants: the fountain of youth?", which evaluated the antioxidant potential of natural juice orange select and pearl pineapple. Previously, a protocol was structured on the practice, which aimed to evaluate the juice antioxidant potential of the fruit orange select and pearl pineapple, which was compared with the antioxidant reference compound, ascorbic acid, with adaptations according to spectrophotometric method in vitro DPPH. The workshops about antioxidants have 180 minutes duration and have been taught by three monitors responsible for assisting students throughout the activity. The workshops involved 388 students from seven schools in six municipalities of Taquari-RS Valley, and the workshop about antioxidants had 62 participants. This activity aimed to promote interaction and diffusion of knowledge among students of higher education and secondary education.Teoria e prática sobre assuntos relacionados à Biotecnologia foram temas de oficinas realizadas por discentes e docentes do Programa de Pós-Graduação em Biotecnologia da UNIVATES a estudantes de Ensino Médio. Foram ministradas dez diferentes oficinas voltadas à produção de alimentos e à saúde, entre as quais destaca-se, neste estudo, a oficina intitulada “Antioxidantes: a fonte da juventude?”, que avaliou o potencial antioxidante do suco natural da laranja seleta e do abacaxi pérola. Previamente, foi estruturado um protocolo sobre a prática, a qual objetivou avaliar o potencial antioxidante do suco das frutas laranja seleta e abacaxi pérola, o qual foi comparado com o composto antioxidante de referência, ácido ascórbico, com adaptações segundo o método espectrofotométrico in vitro DPPH. As oficinas sobre antioxidantes tiveram duração de 180 minutos e foram ministradas por três monitores responsáveis por auxiliar os alunos durante toda a atividade. As oficinas envolveram 388 alunos de sete escolas de seis municípios do Vale do Taquari-RS, sendo que a oficina sobre antioxidantes contou com 62 participantes. Tal atividade buscou promover a interação e difusão de conhecimento entre alunos de Ensino Superior e de Ensino Médio

Adjunctive role of Calyptranthes tricona extract with probiotic Kluyveromyces marxianus on colorectal adenocarcinoma Caco-2 cells

The composition of microbiota may influence the development of colorectal cancer (CRC). In addition, probiotics can decrease the chance of developing cancer or its progress. For that reason, it is encouraging to assess the influence of plant extracts as adjuvants of the health-promoting effects of probiotics. Thus, this study aimed to investigate the in vitro beneficial properties of Calyptranthes tricona leaf ethanol extract in association or not with the lactic yeast Kluyveromyces marxianus on colon adenocarcinoma Caco-2 cells. C. tricona extract inhibited the in vitro p38α MAPK activity and exhibited immunomodulation on isolated human lymphocytes. Further, the extract did not induce cytotoxicity towards K. marxianus or Caco-2 cells; leading to an increased yeast adhesion to cells in a dose-dependent manner. In conclusion, this preliminary study demonstrates that C. tricona extract has effects on enzyme inhibition and immune function. In addition, when associated with K. marxianus, C. tricona may possess beneficial properties for application as enhancer of probiotic’s protective role on CRC cells. However, further studies are necessary in order to elucidate the mechanisms involved.info:eu-repo/semantics/publishedVersio