Modelling biochemical pathways through enhanced π-calculus

AbstractWe use the π-calculus to model the evolution of biochemical systems, taking advantage of their similarities with global computation applications. First, we present a reduction semantics for the π-calculus from which causality and concurrency can be mechanically derived. We prove that our semantics agrees with the causal definitions presented in the literature. We also extend our semantics to model biological compartments. Then, we show the applicability of our proposal on a couple of biological examples

What is New on Thyroid Cancer Biomarkers

Thyroid cancer harbours in about 5% of thyroid nodules. The majority of them are well-differentiated cancers originating from the follicular epithelium, and are subdivided into papillary and follicular carcinomas. Undifferentiated carcinomas and medullary thyroid carcinomas arising from C cells are less common

Cyclosporin A blocks calcium-dependent pathways of gene activation

We have used an interleukin-2 (IL-2) promoter-CAT fusion gene to study activation of IL-2 gene expression by IL-1, phytohemagglutinin (PHA), phorbol myristate acetate (PMA), and calcium ionophore in the murine thymoma line EL4 and the human lymphoma line Jurkat. The two cell lines respond differently to combinations of these stimuli. IL-1 in combination with suboptimal concentration of PMA induced chloramphenicol acetyltransferase (CAT) activity in EL4. In Jurkat cells, IL-1 failed to synergize with PMA or PHA. Cotransfection with the IL-2/CAT gene and a construct capable of expressing murine T-cell type IL-1 receptors converted Jurkat cells to IL-1 responsiveness. IL-1 in combination with PHA but not with PMA resulted in induction of CAT activity in these cells. Induction of IL-2/CAT activity by all stimuli in both cell lines was blocked by the presence of EGTA in the culture medium. EGTA did not inhibit IL-1/PMA activation of an SV40 early promoter-CAT fusion gene in either EL4 or Jurkat cells; therefore, calcium was not required for IL-1 or PMA signal transduction. Jurkat cells were shown to differ from EL4 in their requirement for calcium mobilization. Two different calcium-dependent pathways of gene activation were distinguished, both of which were blocked by the immunosuppressive drug cyclosporin A

Recurrence quantification analysis of heart rate variability to detect both ventilatory thresholds

Aims of this study were: To verify if Recurrence Quantification Analysis (RQA) of Heart Rate Variability (HRV) time series could determine both ventilatory thresholds in individuals with different fitness levels, and to assess the validity of RQA method compared to gasexchange method (GE). The two thresholds were estimated in thirty young individuals during incremental exercise on cycle-ergometer: Heart rate (HR), Oxygen consumption (VO2) and Workload were measured by the two methods (RQA and GE). Repeated measures ANOVA was used to assess main effects of methods and methods-by-groups interaction effects for HR, VO2 and Workload at aerobic (AerT) and anaerobic (AnT) thresholds. Validity of RQA at both thresholds was assessed for HR, VO2 and Workload by Ordinary Least Products (OLP) regression, Typical Percentage Error (TE), Intraclass Correlation Coefficients (ICC) and the Bland Altman plots. No methods-by-groups interaction effects were detected for HR, VO2 and Workload at AerT and AnT. The OLP analysis showed that at both thresholds RQA and GE methods had very strong correlations (r >0.8) in all variables (HR, VO2 and Workload). Slope and intercept values always included the 1 and the 0, respectively. At AerT the TE ranged from 4.02% (5.48 bpm) to 10.47% (8.53 Watts) (HR and Workload, respectively) and in all variables ICC values were excellent (≥0.85). At AnT the TE ranged from 2.53% (3.98 bpm) to 6.64% (7.81 Watts) (HR and Workload, respectively) and in all variables ICC values were excellent (≥0.90). Therefore, RQA of HRV time series is a new valid approach to determine both ventilatory thresholds in individuals with different physical fitness levels, it can be used when gas analysis is not possible or not convenient

Comparação dos procedimentos metodológicos de reconstrução cinemática 2d na técnica de bruços – Duplo meio / planos seprados

O presente estudo teve como propósito principal o de averiguar os procedimentos bem como o grau de concordância das reconstruções dos parâmetros cinemáticos de execução na técnica de bruços com recurso a duas metodologias distintas de captura e reconstrução cinemétrica, duplo meio (DM) e planos separados (PS). A amostra foi constituída por 7 nadadores do sexo masculino especialistas na técnica de bruços (19.1 ± 4,3 anos de idade; 1.78 ± 0.62 m de estatura; 70.4 ± 8.0 kg de massa corporal; 69.10 ± 4.16 s de recorde pessoal aos 100 m Bruços em piscina longa). O protocolo consistiu na realização de um percurso de 100 m na técnica de bruços à máxima velocidade possível (prova maximal), em piscina de 50 metros. Os registos de imagem para posterior tratamento foram recolhidos por um sistema constituído por um par de câmaras de vídeo (uma subaquática - inclusa numa caixa estanque e uma de superfície). Assim, foram recolhidas as imagens da prova utilizando as duas metodologias, o DM e os PS. Duas câmaras registaram respectivamente imagens aéreas (GR-SX1 SVHS, JVC, Yokoama, Japão) e subaquáticas (GR-SXM25 SVHS, JVC, Yokoama, Japão) no plano sagital, a 25 metros da parede testa de partida. Foi efectuada a análise cinemática (Ariel Performance Analysis System, Ariel Dynamics Inc., Califórnia, EUA) de um ciclo gestual completo, através de um leitor de vídeo a 50 Hz (Panasonic, AG 7355, Japão). Recorreu-se ao método de digitalização em planos separados (aéreo e subaquático) e subsequente reconstrução pela aplicação do algoritmo2D-DLT [1]. Foi adoptado o modelo antropométrico de Zatsiorsky, adaptado por de Leva [6],, incluindo a divisão do tronco em 2 partes articuladas. Os sinais foram filtrados com uma frequência de corte de 5 Hz, como sugerido por Winter [15], com recurso a dupla-passagem. A fiabilidade do processo de digitalização-redigitalização para os dois métodos foi muito elevada (R = 0.97; ICR = .87; 0.95). Determinouse a duração absoluta do teste (T100, s), a duração absoluta do ciclo gestual (Durciclo), a frequência ciclo (FC, Hz), a distância de ciclo (DC, m), a velocidade de nado (v, m.s-1), o índice de nado (IN, m2.c-1.s-1), a flutuação intra-cíclica da velocidade horizontal do centro de massa (dv, %) e o deslocamento vertical do centro de massa corporal (Dy, m). Foi efectuada a comparação entre as duas situações com recurso a estatística não paramétrica (Teste de Wilcoxon) para P £ 0.05. O resultados sugerem-nos a ideia de que o padrão biomecânico de execução técnica dos nadadores na técnica de bruços parece ter características diferentes quando comparados os dados dos dois métodos de reconstrução cinemática (DP e PS)

Which physical activity in patients affected by hypoparathyroidism? A review of the literature and practical recommendations

Background Hypoparathyroidism (HypoP) is a rare endocrine condition characterized by hypocalcaemia and hyperphos- phatemia, as a consequence of absent or improperly low parathyroid hormone (PTH) levels. Patients affected by HypoP have a clinical condition often characterized by paresthesias and muscle spasms, as well as long-term consequences as nephro- lithiasis, extraskeletal calcification, and fractures. In the literature, likely due to these symptoms, few data exist regarding the appropriate physical activity (PA) in subjects suffering from HypoP. Purpose This review evaluates the literature on exercise-based approaches to the management of individuals affected by HypoP and evaluates: (1) the effects of physical exercise on muscle cramps and other clinical symptoms; (2) the effects of exercise on PTH and calcium level; (3) the most suitable clinical exercise testing; and (4) the most suitable exercise combination. Methods and results A systematic search was conducted using the databases MEDLINE, Google Scholar using “hypopar- athyroidism AND Physical Activity”, “Training AND hypoparathyroidism”, “Exercise AND muscle cramps”, “Exercise AND Fatigue” as keywords. In addition, references list from the included articles were searched and cross-checked to identify any further potentially eligible studies. A total of 50 manuscripts were found among which 39 manuscripts were selected. A few clinical studies have been performed in HypoP patients to evaluate PA training protocols. Conclusion Although further research is needed to draw solid conclusions regarding best PA protocols in subjects affected by HypoP, a PA protocol has been proposed within the manuscript to encourage patients to attempt exercise to improve their clinical conditions and their quality of life