287 research outputs found
In vitro and in vivo expression of foreign genes by transmissible gastroenteritis coronavirus-derived minigenomes
A helper-dependent expression system based on transmissible gastroenteritis coronavirus (TGEV) has been developed using a minigenome of 3·9 kb (M39). Expression of the reporter gene {beta}-glucuronidase (GUS) (2–8 µg per 106 cells) and the porcine respiratory and reproductive syndrome virus (PRRSV) ORF5 (1–2 µg per 106 cells) has been shown using a TGEV-derived minigenome. GUS expression levels increased about eightfold with the m.o.i. and were maintained for more than eight passages in cell culture. Nevertheless, instability of the GUS and ORF5 subgenomic mRNAs was observed from passages five and four, respectively. About a quarter of the cells in culture expressing the helper virus also produced the reporter gene as determined by studying GUS mRNA production by in situ hybridization or immunodetection to visualize the protein synthesized. Expression of GUS was detected in the lungs, but not in the gut, of swine immunized with the virus vector. Around a quarter of lung cells showing replication of the helper virus were also positive for the reporter gene. Interestingly, strong humoral immune responses to both GUS and PRRSV ORF5 were induced in swine with this virus vector. The large cloning capacity and the tissue specificity of the TGEV-derived minigenomes suggest that these virus vectors are very promising for vaccine development
Dynamic responses of B acteroides thetaiotaomicron during growth on glycan mixtures
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/98163/1/mmi12228-sup-0001-si.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/98163/2/mmi12228.pd
Constructing TI-Friendly Substitution Boxes Using Shift-Invariant Permutations
The threat posed by side channels requires ciphers that can be efficiently protected in both software and hardware against such attacks. In this paper, we proposed a novel Sbox construction based on iterations of shift-invariant quadratic permutations and linear diffusions. Owing to the selected quadratic permutations, all of our Sboxes enable uniform 3-share threshold implementations, which provide first order SCA protections without any fresh randomness. More importantly, because of the shift-invariant property, there are ample implementation trade-offs available, in software as well as hardware. We provide implementation results (software and hardware) for a four-bit and an eight-bit Sbox, which confirm that our constructions are competitive and can be easily adapted to various platforms as claimed. We have successfully verified their resistance to first order attacks based on real acquisitions. Because there are very few studies focusing on software-based threshold implementations, our software implementations might be of independent interest in this regard
Secure Multiplication for Bitslice Higher-Order Masking: Optimisation and Comparison
In this paper, we optimize the performances and compare several recent masking schemes in bitslice on 32-bit arm devices, with a focus on multiplication. Our main conclusion is that efficiency (or randomness) gains always come at a cost, either in terms of composability or in terms of resistance against horizontal attacks. Our evaluations should therefore allow a designer to select a masking scheme based on implementation constraints and security requirements. They also highlight the increasing feasibility of (very) high-order masking that are offered by increasingly powerful embedded devices, with new opportunities of high-security devices in various contexts
Shock absorbing reception surfaces for collecting fruit during the mechanical harvesting of citrus
Damage to fresh citrus caused by impact of fruits onto collecting surfaces has restricted the adoption of mechanical harvesting. Two different experiments were carried out: investigating free-falling citrus and investigating the shock absorbing capacity of various surfaces. In free-falling experiment damage to mandarin, orange and lemon was studied. Three collecting surfaces were studied: a concrete floor, an elevated canvas provided with a frame and wheels, and a concrete floor covered with a shock absorbing canvases. Three dropping heights were used. In the shock absorbing experiment, an electronic sphere and a triaxial accelerometer were used to measure the shock capacity of seven receiving surfaces: earth, earth covered with a shock absorbing canvas, earth covered with weeds, earth covered with a mulch and an elevated canvas provided with a frame and wheels. The elevated canvases had a higher shock absorbing capacity compared to the other surfaces (260 m s−2 maximum acceleration compared with 1753 m s−2 to 2772 m s−2). Weeds, mulch and the shock absorbing canvases showed significantly higher shock absorbing capacity than the bare earth. Also, the shock absorbing canvas covering the concrete floor reduce impact and fruit damage (1866 m s−2 maximum acceleration compared to 2477 m s−2). Citrus damage susceptibility during harvest depended on variety. Weeds, mulch and shock absorbing canvases were shown to reduce impact when they cover earth during the mechanical harvesting of citrus. Elevated canvases could be used as collection systems for the mechanical harvesting of fresh citrus.This study was funded by the Ministerio de Ciencia e Innovacion (research project RTA2009-00118-C02-02) and FEDER. The authors are most grateful to the following collaborators: Maria del Mar Lopez Quevedo, Montano Perez, Juan Jose Pena and Angel Perez.Ortiz Sánchez, MC.; Blasco, J.; Balasch Parisi, S.; Torregrosa Mira, A. (2011). Shock absorbing reception surfaces for collecting fruit during the mechanical harvesting of citrus. Biosystems Engineering. 110(1):2-9. https://doi.org/10.1016/j.biosystemseng.2011.05.006S29110
Actuación en zonas antiguas de pueblos y ciudades
Actuación en zonas antiguas de pueblos y ciudade
Milk yield estimation during suckling using the double oxytocin injection-milking and the double weighing-suckling methods in dairy goats
The aim was to verify the validity of the double oxytocin-milking (DOT) method as milk
yield estimator during the suckling period of lactating dairy goats. To this end, it was necessary
to determine whether the weighing suckling weighing (WSW) and DOT methods of
milk yield estimation satisfied the criteria to be considered valuable, the accuracy between
both methods and the suitability of DOT to evaluate actual milk. At parturition, sixty lactating
Murciano Granadina breed goats were separated into 2 groups, in mixed (MS; n = 24)
and artificial rearing (ARS; n = 36) management systems. Until the sixth week of lactation
(weaning), MS goats suckled one kid while kids from ARS goats were artificially reared;
moreover, goats in both systems were submitted to once-a-day milking. Once per week,
actual milk yield for ARS goats and potential milk yield were recorded using DOT method
for all goats, except for 12 goats in ARS which remained as a control. Twelve goats from each
management system were used to evaluate diurnal variation in milk production (DVM) by
DOT method for 6 consecutive days in week 4 of lactation. No difference in DVM was found
by DOT method in 4-h milk production of goats in MS (P = 0.099) or ARS (P = 0.220), which
allowed sixfold multiplication of milked milk volume to obtain potential milk per day. ARS
goats subjected to a weekly DOT and control group goats showed a similar (P = 0.379) lactation
curve for the first 6 weeks of lactation. The DOT method slightly overestimated (3.4%,
P = 0.005) the milk yield evaluated by WSW method for goats under an MS, but fitted the
actual milk obtained by common milk records for the group of goats in an ARS submitted
to the DVM test (P = 0.357) and the group in ARS alone (P = 0.163). The DOT method applied
for 8 consecutive days led to a drop of 6 12% in milk yield during the following week for
both production systems. In conclusion, DOT was an accurate method to estimate milk
yield during the first weeks of lactation both in MS and ARS under the conditions of this
experiment.
© 2013 Elsevier B.V. All rights reserved.Fernández Martínez, N.; Balasch Parisi, S.; Pérez Baena, I.; Rodríguez Garcia, M.; Peris Ribera, CJ. (2013). Milk yield estimation during suckling using the double oxytocin injection-milking and the double weighing-suckling methods in dairy goats. Small Ruminant Research. 112(1-3):181-185. doi:10.1016/j.smallrumres.2012.12.023S1811851121-
Machine milking parameters for Murciano-Granadina breed goats
[EN] In dairy ruminants, the combination of milking parameters must ensure good milking performance without harming udder conditions. Commonly, milking conditions for goats are established without having checked the admissible limits for optimal and fast milking. The aim of this study was to establish a limit combination of machine milking parameters that improves machine milking performance without altering milkability or udder status. To this end, we studied the effect of 2 combinations (42 kPa, 120 cpm, 60% vs. 44 kPa, 120 cpm, 60% in terms of kilopascals of vacuum level, cycles per minute of pulsator rate, and percentage of pulsator ratio, respectively) on milk production and composition, milk fractioning during milking, SCC, teat tissue thickness variation after milking, and the milk emission kinetics parameters throughout 1 lactation period (6 rno). The 42 and 44 kPa measured at the vacuum gauge level became average values of 37.5 and 39.3 kPa, respectively, measured at the teat sphincter level during milking. Milk flow significantly increased and total milking time decreased 25 s with the elevation of the vacuum level from 42 to 44 kPa without any adverse effect on milk fractioning at milking. However, the use of 44 kPa also showed an increase in tissue thickness above 5%, and we observed a tendency of average conductivity of milk to increase, although without any adverse effect on SCC. It seems that 44 kPa, 120 cpm, 60% is a possible limit combination of parameters to improve milking performance without altering milkability or udder conditions. We concluded that this combination can be used for milking Murciano-Granadina breed goats in conditions similar to those of this study (mid-level milking system and 1 milking/d), although further studies are necessary to verify its application in the case of 2 milkings/d.Fernández Martínez, N.; Martí Vicent, JV.; Rodríguez Garcia, M.; Peris Ribera, CJ.; Balasch Parisi, S. (2020). Machine milking parameters for Murciano-Granadina breed goats. Journal of Dairy Science. 103(1):507-513. https://doi.org/10.3168/jds.2019-16446S5075131031Alejandro, M., Roca, A., Romero, G., & Díaz, J. R. (2014). Short communication: Effects of milk removal on teat tissue and recovery in Murciano-Granadina goats. Journal of Dairy Science, 97(8), 5012-5016. doi:10.3168/jds.2014-7934Ali, A. K. A., & Shook, G. E. (1980). An Optimum Transformation for Somatic Cell Concentration in Milk. Journal of Dairy Science, 63(3), 487-490. doi:10.3168/jds.s0022-0302(80)82959-6Blasco, E., Gomez, E. A., Vicente, C., Vidal, G., & Peris, C. (2016). Factors affecting milking speed in Murciano-Granadina breed goats. Journal of Dairy Science, 99(12), 10102-10108. doi:10.3168/jds.2016-10869BOE (Boletín Oficial del Estado). 2013. Real Decreto 53/2013, de 1 de febrero, por el que se establecen las normas básicas aplicables para la protección de los animales utilizados en experimentación y otros fines científicos, incluyendo la docencia.Delgado-Pertíñez, M., Guzmán-Guerrero, J. L., Mena, Y., Castel, J. M., González-Redondo, P., & Caravaca, F. P. (2009). Influence of kid rearing systems on milk yield, kid growth and cost of Florida dairy goats. Small Ruminant Research, 81(2-3), 105-111. doi:10.1016/j.smallrumres.2008.12.007Díaz, J. R., Alejandro, M., Peris, C., & Fernández, N. (2013). Use of ultrasound scanning to estimate teat wall thickness in Murciano-Granadina goats. Livestock Science, 155(1), 114-122. doi:10.1016/j.livsci.2013.04.007Díaz, J. R., Romero, G., Muelas, R., Alejandro, M., & Peris, C. (2012). Effect of intramammary infection on milk electrical conductivity in Murciano-Granadina goats. Journal of Dairy Science, 95(2), 718-726. doi:10.3168/jds.2011-4698Diaz, J. R., Romero, G., Muelas, R., Sendra, E., Pantoja, J. C. F., & Paredes, C. (2011). Analysis of the influence of variation factors on electrical conductivity of milk in Murciano-Granadina goats. Journal of Dairy Science, 94(8), 3885-3894. doi:10.3168/jds.2011-4187Fernández, N., Martínez, A., Martí, J. V., Rodríguez, M., & Peris, C. (2015). Milkability and milking efficiency improvement in Murciano-Granadina breed goats. Small Ruminant Research, 126, 68-72. doi:10.1016/j.smallrumres.2015.03.001Gil, Z. (1988). Milk temperature fluctuations during milking in cows with subclinical mastitis. Livestock Production Science, 20(3), 223-231. doi:10.1016/0301-6226(88)90074-7Hamann, J., Mein, G. A., & Nipp, B. (1996). Recommended method for measuring changes in thickness of the bovine teat with spring-loaded calipers. Journal of Dairy Research, 63(2), 309-313. doi:10.1017/s0022029900031800Hamzaoui, S., Salama, A. A. K., Albanell, E., Such, X., & Caja, G. (2013). Physiological responses and lactational performances of late-lactation dairy goats under heat stress conditions. Journal of Dairy Science, 96(10), 6355-6365. doi:10.3168/jds.2013-6665Isaksson, A., & Lind, O. (1992). Teat Reactions in Cows Associated With Machine Milking*. Journal of Veterinary Medicine Series A, 39(1-10), 282-288. doi:10.1111/j.1439-0442.1992.tb00184.xLE DU, J., BENMEDERBEL, B., DANO, Y., COMBAUD, J. F., & TESSONNIÈRE, R. (1984). Aptitude des chèvres de race Saanen à la traite mécanique. Relations avec les caractéristiques physiques du trayon. Annales de Zootechnie, 33(3), 375-384. doi:10.1051/animres:19840307Peris, S., Caja, G., Such, X., Casals, R., Ferret, A., & Torre, C. (1997). Influence of Kid Rearing Systems on Milk Composition and Yield of Murciano-Granadina Dairy Goats. Journal of Dairy Science, 80(12), 3249-3255. doi:10.3168/jds.s0022-0302(97)76299-4Requena, R., Balasch, S., Peris, C., Rodríguez, M., & Fernández, N. (2010). Dose response of lactating dairy ewes during suckling and milking to bovine somatotropin1. Journal of Animal Science, 88(9), 3136-3144. doi:10.2527/jas.2009-2690Zecconi, A., Hamann, J., Bronzo, V., & Ruffo, G. (1992). Machine-induced teat tissue reactions and infection risk in a dairy herd free from contagious mastitis pathogens. Journal of Dairy Research, 59(3), 265-271. doi:10.1017/s002202990003054
A comparison of menotropin, highly-purified menotropin and follitropin alfa in cycles of intracytoplasmic sperm injection
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