34 research outputs found
Role of Operating Conditions on Energetic Pathways in a Microbial Fuel Cell
AbstractThe electric performance of a Microbial Fuel Cell (MFC) fed with swine manure, and specifically the interactions between different coexisting bacterial populations are examined in relationship to the Organic Loading Rate (OLR) and External Resistance applied to the cell. Feasibility of swine manure treatment using MFCs was already demonstrated by previous studies, however low Coulombic efficiencies were attained due to a competing methanogenic degradation occurring in the same cells. External resistance (Rext) and Organic Loading Rate have been identified as two of the key parameters affecting the balance between exoelectrogenic and methanogenic bacterial populations in a MFC system; despite this, virtually no attention had been paid to the study of OLR influence on MFCs performance. This study evaluates the performance of a MFC, treating swine manure, in this perspective, demonstrating that high OLRs (up to 11.2kg COD m3/d) have a limiting effect on MFCs electrochemical losses, and increase absolute values of ORR (4.6kg COD m3/d) and current production (14.9mA). On the other hand, adoption of low OLR (as low as 0.7kg COD m3/d) translates in an increase of both organic matter removal efficiency (52%) and Coulombic efficiency (higher than 70%). These improvements can be directly connected with the shifting balance between exoelectrogenic and methanogenic biomass populations, as confirmed by the cell's anode off-gas analysis. Hence, by adopting the appropriate design value of ORL and operating conditions, the MFC's biofilm exoelectrogenic population fraction, and thus its overall activity, can be improved considerably
Assessment of microbial community structure changes by amplified ribosomal DNA restriction analysis (ARDRA)
Amplified ribosomal DNA restriction analysis (ARDRA) is a simple method based on restriction endonuclease digestion of the amplified bacterial 16S rDNA. In this study we have evaluated the suitability of this method to detect differences in activated sludge bacterial communities fed on domestic or industrial wastewater, and subject to different operational conditions. The ability of ARDRA to detect these differences has been tested in modified Ludzack-Ettinger (MLE) configurations. Samples from three activated sludge wastewater treatment plants (WWTPs) with the MLE configuration were collected for both oxic and anoxic reactors, and ARDRA patterns using double enzyme digestions AluI+MspI were obtained. A matrix of Dice similarity coefficients was calculated and used to compare these restriction patterns. Differences in the community structure due to influent characteristics and temperature could be observed, but not between the oxic and anoxic reactors of each of the three MLE configurations. Other possible applications of ARDRA for detecting and monitoring changes in activated sludge systems are also discussed
Effect of hydraulic retention time on the electro-bioremediation of nitrate in saline groundwater
Bioelectrochemical systems (BES) have proven their capability to treat nitrate-contaminated saline groundwater and simultaneously recover value-added chemicals (such as disinfection products) within a circular economy-based approach. In this study, the effect of the hydraulic retention time (HRT) on nitrate and salinity removal, as well as on free chlorine production, was investigated in a 3-compartment BES working in galvanostatic mode with the perspective of process intensification and future scale-up. Reducing the HRT from 30.1 +/- 2.3 to 2.4 +/- 0.2 h led to a corresponding increase in nitrate removal rates (from 17 +/- 1 up to 131 +/- 1 mgNO3--N L-1d-1), although a progressive decrease in desalination efficiency (from 77 +/- 13 to 12 +/- 2 %) was observed. Nitrate concentration and salinity close to threshold limits indicated by the World Health Organization for drinking water, as well as significant chlorine production were achieved with an HRT of 4.9 +/- 0.4 h. At such HRT, specific energy consumption was low (6.8 center dot 10-2 +/- 0.3 center dot 10-2 kWh g-1NO3--Nremoved), considering that the supplied energy supports three processes simultaneously. A logarithmic equation correlated well with nitrate removal rates at the applied HRTs and may be used to predict BES behaviour with different HRTs. The bacterial community of the bio-cathode under galvanostatic mode was dominated by a few populations, including the genera Rhizobium, Bosea, Fontibacter and Gordonia. The results provide useful information for the scale-up of BES treating multi-contaminated groundwater
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Este volumen reúne los textos de algunas de las conferencias que se pronunciaron durante el curso "Nuevos enfoques teóricos y metodológicos II" impartido en la Universidad de Barcelona en 1993. Las conferencias formaron parte del segundo programa de doctorado Mujeres, Género y Poder, organizado desde el Departamento de Antropología e Historia de América a través del Seminario Interdisciplinar Mujeres y Sociedad