Competence, specification and induction of Pax-3 in the trigeminal placode

Placodes are discrete regions of thickened ectoderm that contribute extensively to the peripheral nervous system in the vertebrate head. The paired-domain transcription factor Pax-3 is an early molecular marker for the avian ophthalmic trigeminal (opV) placode, which forms sensory neurons in the ophthalmic lobe of the trigeminal ganglion. Here, we use collagen gel cultures and heterotopic quail-chick grafts to examine the competence, specification and induction of Pax-3 in the opV placode. At the 3-somite stage, the whole head ectoderm rostral to the first somite is competent to express Pax-3 when grafted to the opV placode region, though competence is rapidly lost thereafter in otic-level ectoderm. Pax-3 specification in presumptive opV placode ectoderm occurs by the 8-somite stage, concomitant with robust Pax-3 expression. From the 8-somite stage onwards, significant numbers of cells are committed to express Pax-3. The entire length of the neural tube has the ability to induce Pax-3 expression in competent head ectoderm and the inductive interaction is direct. We propose a detailed model for Pax-3 induction in the opV placode

High frequency GaAs nano-optomechanical disk resonator

Optomechanical coupling between a mechanical oscillator and light trapped in a cavity increases when the coupling takes place in a reduced volume. Here we demonstrate a GaAs semiconductor optomechanical disk system where both optical and mechanical energy can be confined in a sub-micron scale interaction volume. We observe giant optomechanical coupling rate up to 100 GHz/nm involving picogram mass mechanical modes with frequency between 100 MHz and 1 GHz. The mechanical modes are singled-out measuring their dispersion as a function of disk geometry. Their Brownian motion is optically resolved with a sensitivity of 10^(-17)m/sqrt(Hz) at room temperature and pressure, approaching the quantum limit imprecision.Comment: 7 pages, 3 figure

Modelling of spin decoherence in a Si hole qubit perturbed by a single charge fluctuator

Spin qubits in semiconductor quantum dots are one of the promizing devices to realize a quantum processor. A better knowledge of the noise sources affecting the coherence of such a qubit is therefore of prime importance. In this work, we study the effect of telegraphic noise induced by the fluctuation of a single electric charge. We simulate as realistically as possible a hole spin qubit in a quantum dot defined electrostatically by a set of gates along a silicon nanowire channel. Calculations combining Poisson and time-dependent Schr\"odinger equations allow to simulate the relaxation and the dephasing of the hole spin as a function of time for a classical random telegraph signal. We show that dephasing time $T_2$ is well given by a two-level model in a wide range of frequency. Remarkably, in the most realistic configuration of a low frequency fluctuator, the system has a non-Gaussian behavior in which the phase coherence is lost as soon as the fluctuator has changed state. The Gaussian description becomes valid only beyond a threshold frequency $\omega_{th}$, when the two-level system reacts to the statistical distribution of the fluctuator states. We show that the dephasing time $T_{2}(\omega_{th})$ at this threshold frequency can be considerably increased by playing on the orientation of the magnetic field and the gate potentials, by running the qubit along "sweet" lines. However, $T_{2}(\omega_{th})$ remains bounded due to dephasing induced by the non-diagonal terms of the stochastic perturbation Hamiltonian. Our simulations reveal that the spin relaxation cannot be described cleanly in the two-level model because the coupling to higher energy hole levels impacts very strongly the spin decoherence. This result suggests that multi-level simulations including the coupling to phonons should be necessary to describe the relaxation phenomenon in this type of qubit

Wavelength-sized GaAs optomechanical resonators with GHz frequency

We report on wavelength-sized GaAs optomechanical disk resonators showing ultra-strong optomechanical interaction. We observe optical transduction of a disk mechanical breathing mode with 1.4 GHz frequency and effective mass of ~ 2 pg. The measured vacuum optomechanical coupling rate reaches 0.8 MHz, with a related differential optomechanical coupling factor of 485 GHz/nm. The disk Brownian motion is optically resolved with a sensitivity of 10-17 m/{\sqrt}Hz at room temperature and pressure.Comment: 8 pages, 3 figure

Origin of optical losses in gallium arsenide disk whispering gallery resonators

Whispering gallery modes in GaAs disk resonators reach half a million of optical quality factor. These high Qs remain still well below the ultimate design limit set by bending losses. Here we investigate the origin of residual optical dissipation in these devices. A Transmission Electron Microscope analysis is combined with an improved Volume Current Method to precisely quantify optical scattering losses by roughness and waviness of the structures, and gauge their importance relative to intrinsic material and radiation losses. The analysis also provides a qualitative description of the surface reconstruction layer, whose optical absorption is then revealed by comparing spectroscopy experiments in air and in different liquids. Other linear and nonlinear optical loss channels in the disks are evaluated likewise. Routes are given to further improve the performances of these miniature GaAs cavities.Comment: 18 page

Biomonitoring of 2,4-Dichlorophenoxyacetic Acid Exposure and Dose in Farm Families

OBJECTIVE: We estimated 2,4-dichlorophenoxyacetic acid (2,4-D) exposure and systemic dose in farm family members following an application of 2,4-D on their farm. METHODS: Farm families were recruited from licensed applicators in Minnesota and South Carolina. Eligible family members collected all urine during five 24-hr intervals, 1 day before through 3 days after an application of 2,4-D. Exposure profiles were characterized with 24-hr urine 2,4-D concentrations, which then were related to potential predictors of exposure. Systemic dose was estimated using the urine collections from the application day through the third day after application. RESULTS: Median urine 2,4-D concentrations at baseline and day after application were 2.1 and 73.1 μ g/L for applicators, below the limit of detection, and 1.2 μ g/L for spouses, and 1.5 and 2.9 μ g/L for children. The younger children (4–11 years of age) had higher median post-application concentrations than the older children (≥ 12 years of age) (6.5 vs. 1.9 μ g/L). The geometric mean systemic doses (micrograms per kilogram body weight) were 2.46 (applicators), 0.8 (spouses), 0.22 (all children), 0.32 (children 4–11 years of age), and 0.12 (children ≥ 12 years of age). Exposure to the spouses and children was primarily determined by direct contact with the application process and the number of acres treated. Multivariate models identified glove use, repairing equipment, and number of acres treated as predictors of exposure in the applicators. CONCLUSIONS: We observed considerable heterogeneity of 2,4-D exposure among farm family members, primarily attributable to level of contact with the application process. Awareness of this variability and the actual magnitude of exposures are important for developing exposure and risk characterizations in 2,4-D–exposed agricultural populations

Recognition of leitmotives in Richard Wagner's music: chroma distance and listener expertise

The leitmotives in Richard Wagner’s Der Ring des Nibelungen serve a range of compositional and psychological functions, including the introduction of musical structure and mnemonic devices for the listener. Leitmotives in the Ring differ greatly in their construction, salient aspects (e.g. rhythmic, melodic, harmonic), and their usage in particular scenes and contexts. We aim to understand listeners’ real-time processing of leitmotives, and have gathered data from a memory test, probing participants’ memory for different leit- motives contained in a 10-minute excerpt from the opera Siegfried. An item response theory (IRT) approach was used to estimate item difficulty parameters as well as parameters characterizing participants’ individual recognition ability. We fit a series of IRT models to the data obtained from 68 participants, finding that a Rasch Model with an unconstrained but fixed discrimination parameter fit the data best accord- ing to the Bayesian Information Criterion. We further investigated the relationship between model parameters and factors such as: number of leitmotive occurrences in the excerpt; acoustical distance using chroma features (Mauch & Dixon, 2010) and distance thresholding (Casey, Rhodes & Slaney, 2008); extent of musical training; and objective and self-reported Wagner expertise, finding that performance in the objective Wagner test and chroma distance were statistically significant predictors, while number of occurrences, self-reported Wagner expertise and extent of musical training did not reach significance

Holocene variations in Lake Titicaca water level and their implications for sociopolitical developments in the central Andes

Holocene climate in the high tropical Andes was characterized by both gradual and abrupt changes, which disrupted the hydrological cycle and impacted landscapes and societies. High-resolution paleoenvironmental records are essential to contextualize archaeological data and to evaluate the sociopolitical response of ancient societies to environmental variability. Middle-to-Late Holocene water levels in Lake Titicaca were reevaluated through a transfer function model based on measurements of organic carbon stable isotopes, combined with high-resolution profiles of other geochemical variables and paleoshoreline indicators. Our reconstruction indicates that following a prolonged low stand during the Middle Holocene (4000 to 2400 BCE), lake level rose rapidly ~15 m by 1800 BCE, and then increased another 3 to 6 m in a series of steps, attaining the highest values after ~1600 CE. The largest lake-level increases coincided with major sociopolitical changes reported by archaeologists. In particular, at the end of the Formative Period (500 CE), a major lake-level rise inundated large shoreline areas and forced populations to migrate to higher elevation, likely contributing to the emergence of the Tiwanaku culture