Supplementary data for article: Andjelković, U.; Milutinović-Nikolić, A.; Jović-Jovičić, N.; Banković, P.; Bajt, T.; Mojović, Z.; Vujčić, Z.; Jovanović, D. Efficient Stabilization of Saccharomyces Cerevisiae External Invertase by Immobilisation on Modified Beidellite Nanoclays. Food Chemistry 2015, 168, 262–269. https://doi.org/10.1016/j.foodchem.2014.07.055

The external invertase isoform 1 (EINV1) was immobilised on eight differently modified beidellite nanoclays, Modifications were composed of organo-modification with different amounts of surfactant - hexadecyl trimethylammonium cation (HDTMA), pillaring with Al/Fe containing polyhydroxy cations and acid modification of Na-enriched and pillared clays. The modified nanoclays were characterised by XRD, N-2-physisorption, SEM and FT-IR spectroscopy. The amount of bound enzyme activity was significantly influenced by the modification of beidellite ranging from 50 to remarkable 2200 U/g. Biochemical characterization was performed for five modified nanoclays showing the highest enzyme activity after invertase immobilisation. The investigation demonstrated that after immobilisation the structure and the catalytic properties of invertase were preserved, while Km values were slightly increased from 26 to 37 mM. immobilisation significantly improved thermal and storage stability of EINV1. Results indicate that beidellite nanoclays obtained by low cost modifications can be applied as a suitable support for the immobilisation of invertase. The immobilizate can be efficiently engaged in sucrose hydrolysis in batch reactor.Supplementary material for: [https://doi.org/10.1016/j.foodchem.2014.07.055]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1865

A comparison of different cytotoxicity assays for determination of antitumour effect of chemoterapeutic cis-diamminedichloridoplatinum (II)

Cancer is becoming a major health problem both in Slovenia and worldwide, as reported in Slovenian National Programme for Cancer Management. According to epidemiological predictions, cancer will soon become the first problem of modern society. Therefore, the progress in treatment development is of crucial importance. Options for cancer treatment include local management, such as surgical oncology and radiation therapy, and systemic management, such as chemotherapy, hormonal therapy and treatment with biotherapeutic agents. Overall progress of medical treatment for various diseases can be seen also in cancer treatment based on chemotherapy. In the last two decades, new advanced knowledge of molecular and cellular biology led to improved therapy. Progress in the understanding of cancer disease coincides with the progress in the evaluation of new anticancer compounds using various screening assays. When developing new screening assays, it is also important to evaluate and compare these assays with the established standard assays to evaluate their efficiency. In our study, we compared the standard clonogenic assay with MTT assay and Presto Blue assay, commonly used for large scale screening of cytotoxic compounds by evaluating cellular cytotoxicity. Our results have demonstrated a good correlation in sensitivity according to the measured IC50 values between clonogenic assay, MTT assay and Presto Blue assay. MTT and Presto Blue assays differ in sensitivity, as sensitivity of Presto Blue assay is higher than sensitivity of MTT assay

Project 1: “Towards a prophylactic Epstein Barr Virus (EBV) vaccine to prevent EBV -associated malignancies and infectious mononucleosis” and project 2: “Analysing the transcriptional response to histone deacetylase inhibitors treatment in normal and cancer cell lines”

The combined thesis submitted for award of the Master of Research in Molecular and Cellular Biology consists of two projects. The first project with the title “towards a prophylactic Epstein Barr Virus (EBV) vaccine to prevent EBV-associated malignancies and infectious mononucleosis” aim was to investigate T cell responses in healthy EBV-seropositive donors to two EBV structural proteins MCP, encoded by BcLF1, and LTP, encoded by BPLF1. The results of first project showed that EBV proteins MCP and LTP are a good vaccine candidates. The second project with the title “analysing the transcriptional response to histone deacetylase inhibitors treatment in normal and cancer cell lines” aim was to analyse the transcriptional response to histone deacetylase inhibitor valproic acid (VPA) treatment in normal and cancer cell lines. The results of second project showed that VPA is not as toxic to normal cells as other chemotherapeutic agents and is a promising candidate for advanced epigenetic chemotherapy

Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays

The external invertase isoform 1 (EINV1) was immobilised on eight differently modified beidellite nanoclays, Modifications were composed of organo-modification with different amounts of surfactant - hexadecyl trimethylammonium cation (HDTMA), pillaring with Al/Fe containing polyhydroxy cations and acid modification of Na-enriched and pillared clays. The modified nanoclays were characterised by XRD, N-2-physisorption, SEM and FT-IR spectroscopy. The amount of bound enzyme activity was significantly influenced by the modification of beidellite ranging from 50 to remarkable 2200 U/g. Biochemical characterization was performed for five modified nanoclays showing the highest enzyme activity after invertase immobilisation. The investigation demonstrated that after immobilisation the structure and the catalytic properties of invertase were preserved, while Km values were slightly increased from 26 to 37 mM. immobilisation significantly improved thermal and storage stability of EINV1. Results indicate that beidellite nanoclays obtained by low cost modifications can be applied as a suitable support for the immobilisation of invertase. The immobilizate can be efficiently engaged in sucrose hydrolysis in batch reactor.Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3365