Estructura genética poblacional de dos especies piscícolas, una de estuario y una de arrecife, explotados por la pesca artesanal brasilera

In this study, we used microsatellite markers to examine the genetic structures of Centropomus undecimalis (Bloch, 1792) and Epinephelus marginatus (Lowe, 1834) populations collected from artisanal fishing sites along a stretch of coastline in southeastern Brazil. Based on F-statistics, there was no significant genetic differentiation evident in any C. undecimalis samples (FST=0.012). However, Bayesian clustering, principal component analysis (PCA) and discriminant analysis of principal components (DAPC) results suggested that there were most likely two clusters, with no relation to geographic areas. The bottleneck results showed no significant values and the effective population sizes (Ne) for the two genetically differentiated groups were large and similar. In contrast, for E. marginatus populations, the microsatellite loci showed no population subdivisions. The FST value was low and non-significant (FST=0.008), a Bayesian analysis indicated one cluster, and a PCA showed that all samples from different geographical sites shared the same genetic structure. The bottleneck results exhibited significant differences, and a low Ne was observed. The results of the genetic study of these two species along the southeastern Brazilian coast suggest that the distinct genetic structure of each species should be taken into account as management units for the conservation of their genetic diversities.En este estudio se han utilizado marcadores microsatélites para examinar la estructura genética de Centropomus undecimalis (Bloch, 1792) y Epinephelus marginatus (Lowe, 1834) en localidades de pesca artesanal localizadas a lo largo de la costa del sudeste de Brasil. Los estadísticos de diferenciación poblacional (F-statisctics) no presentaron diferenciación genética entre las muestras de C. undecimalis (FST=0.012). Sin embargo, los resultados de los análisis basados en clústeres bayesianos, componentes principales (PCA) y análisis discriminante de componentes principales (DAPC) sugieren la posible presencia de dos clústeres diferenciados genéticamente, pero sin ninguna relación con las áreas geográficas. Los resultados del análisis de cuello de botella poblacional no son significativos con valores de tamaño efectivo poblacional (Ne) elevados y similares entre los dos clústeres. Por el contrario, en E. marginatus, los análisis basados en microsatélites no muestran ningún patrón de subdivisión genética. El valor de FST es bajo y no significativo (FST=0.008), el análisis Bayesiano indicia un solo clúster y el PCA determina que todas las muestras de las diferentes localidades geográficas comparten la misma estructura genética. El análisis de cuello de botella muestra diferencias significativas con la observación de una baja Ne. Los resultados de los análisis genéticos en estas dos especies a lo largo de la costa sudeste de Brasil sugieren diferentes estructuras genéticas para cada especie y estos resultados deben tenerse en consideración para la estipulación de las medidas de conservación de la diversidad genética

Microsatellite markers isolated from Cabomba Aquatica S.l. (cabombaceae) from an enriched genomic library

Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters. Using a selective hybridization method, we designed and tested 30 simple sequence repeat loci using two natural populations of C. aquatica s.l., resulting in 13 amplifiable loci. Twelve loci were polymorphic, and alleles per locus ranged from two to four across the 49 C. aquatica s.l. individuals. Observed heterozygosity, expected heterozygosity, and fixation index varied from 0.0 to 1.0, 0.0 to 0.5, and -1.0 to -0.0667, respectively, for the Manaus population and from 0.0 to 1.0, 0.0 to 0.6, and -1.0 to 0.4643 for the Viruá population. The developed markers will be used in further taxonomic and population studies within Cabomba. This set of microsatellite primers represents the first report on rapid molecular markers in the genus.Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters. Using a selective hybridization method, we designed and tested 30 simple sequence repeat l31113CNQP - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORSEM INFORMAÇÃO457/2010The authors thank S. M. Costa for help during fieldwork, the staff of the Parque Nacional do Viruá and Instituto Nacional de Pesquisas da Amazônia (INPA) for field support, and A. P. Souza for technical support for the laboratory infrastructure. T.D.M.B.

Development and characterization of microsatellite markers for Piptadenia Gonoacantha (fabaceae)

Microsatellite primers were designed for Piptadenia gonoacantha (Fabaceae) and characterized to estimate genetic diversity parameters. The species is a native tree from the Atlantic Forest biome commonly used in forest restoration; it has medicinal potential and the wood is economically useful. Twenty-eight microsatellite loci were identified from an enriched genomic library. Fifteen loci resulted in successful amplifications and were characterized in a natural population of 94 individuals. Twelve loci were polymorphic, with allele numbers ranging from three to 15 per locus, and expected and observed heterozygosities ranging from 0.2142 to 0.8325 and 0.190 to 0.769, respectively. The developed markers will be used in further studies of population genetics of P. gonoacantha, aimed at conservation and management of the species in natural populations and in forest restoration projects32CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP140036/2011-3sem informaçãoBiota/FAPESP-11/50296-

New loci of Lychnophora ericoides and transferability to Lychnophora pinaster, endangered medicinal species from Brazil

Lychnophora ericoides and Lychnophora pinaster are species used in popular medicine as analgesic or anti-inflammatory agents to treat contusions, rheumatism, and insect bites. In this study, 21 simple sequence repeat loci of L. ericoides were developed and transferred to L. pinaster. Three populations of L. ericoides and 2 populations of L. pinaster were evaluated; they were collected in the State of Minas Gerais. Population parameters were estimated, and the mean values of observed and expected heterozygosity were 0.297 and 0.408 (L. ericoides) and 0.228 and 0.310 (L. pinaster), respectively. Greater genetic variability was observed within populations than between populations of L. ericoides (62 and 37%) and L. pinaster (97 and 2.8%). These results provide information for genetic conservation and taxonomic studies of these endangered species. Lychnophora ericoides and Lychnophora pinaster are species used in popular medicine as analgesic or anti-inflammatory agents to treat contusions, rheumatism, and insect bites. In this study, 21 simple sequence repeat loci of L. ericoides were developed and transferred to L. pinaster. Three populations of L. ericoides and 2 populations of L. pinaster were evaluated; they were collected in the State of Minas Gerais. Population parameters were estimated, and the mean values of observed and expected heterozygosity were 0.297 and 0.408 (L. ericoides) and 0.228 and 0.310 (L. pinaster), respectively. Greater genetic variability was observed within populations than between populations of L. ericoides (62 and 37%) and L. pinaster (97 and 2.8%). These results provide information for genetic conservation and taxonomic studies of these endangered species1341087810882sem informaçã

Microsatellite markers for the Cabreúva tree, Myroxylon peruiferum (Fabaceae), an endangered medicinal species from the Brazilian Atlantic Forest

The Cabreúva tree, Myroxylon peruiferum, is an endangered tropical species from Brazil used in forest restoration projects. It is known for its medicinal properties. Eleven microsatellite markers were developed for this species, from a microsatellite-enriched library. Nine of these markers, characterized in 30 individuals from a semideciduous forest remnant population in southeast Brazil, were polymorphic, with allele numbers ranging from 2 to 8 per locus; expected and observed heterozygosities ranged from 0.103 to 0.757 and 0.107 to 0.704, respectively. One locus (Mpe-C04) showed significant deviation from Hardy-Weinberg equilibrium, probably due to null alleles. Two other loci (Mpe-E09 and Mpe-H07) were monomorphic in this population. These microsatellite loci should be useful for future population genetic studies of this species. The Cabreúva tree, Myroxylon peruiferum, is an endangered tropical species from Brazil used in forest restoration projects. It is known for its medicinal properties. Eleven microsatellite markers were developed for this species, from a microsatellite-enriched library. Nine of these markers, characterized in 30 individuals from a semideciduous forest remnant population in southeast Brazil, were polymorphic, with allele numbers ranging from 2 to 8 per locus; expected and observed heterozygosities ranged from 0.103 to 0.757 and 0.107 to 0.704, respectively. One locus (Mpe-C04) showed significant deviation from Hardy-Weinberg equilibrium, probably due to null alleles. Two other loci (Mpe-E09 and Mpe-H07) were monomorphic in this population. These microsatellite loci should be useful for future population genetic studies of this species13369206925CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPsem informaçã

Microsatellite Markers For The Cabreúva Tree, Myroxylon Peruiferum (fabaceae), An Endangered Medicinal Species From The Brazilian Atlantic Forest.

The Cabreúva tree, Myroxylon peruiferum, is an endangered tropical species from Brazil used in forest restoration projects. It is known for its medicinal properties. Eleven microsatellite markers were developed for this species, from a microsatellite-enriched library. Nine of these markers, characterized in 30 individuals from a semideciduous forest remnant population in southeast Brazil, were polymorphic, with allele numbers ranging from 2 to 8 per locus; expected and observed heterozygosities ranged from 0.103 to 0.757 and 0.107 to 0.704, respectively. One locus (Mpe-C04) showed significant deviation from Hardy-Weinberg equilibrium, probably due to null alleles. Two other loci (Mpe-E09 and Mpe-H07) were monomorphic in this population. These microsatellite loci should be useful for future population genetic studies of this species.136920-

Viabilidade econômica da produção de eucalipto no polo moveleiro de Marco - Ceará.

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Genômica populacional e introgressão gênica em rebanhos caprino naturalmente adaptado ao Brasil.

The aim of this study was to apply the Illumina 50 K goat SNP Chip to analyse population genomic structure in two herds of Marota goats in the State of Piauí, one private and the other an official conservation herd, and to investigate evidence of genetic erosion in these herds caused by the Anglo-Nubian goat. To that end, 86 Marota and 10 Anglo-Nubian animals were genotyped. Genetic diversity was analysed by comparing minor allele frequency (MAF) in the herds