A STOCHASTIC SIMULATION-BASED HYBRID INTERVAL FUZZY PROGRAMMING APPROACH FOR OPTIMIZING THE TREATMENT OF RECOVERED OILY WATER

In this paper, a stochastic simulation-based hybrid interval fuzzy programming (SHIFP) approach is developed to aid the decision-making process by solving fuzzy linear optimization problems. Fuzzy set theory, probability theory, and interval analysis are integrated to take into account the effect of imprecise information, subjective judgment, and variable environmental conditions. A case study related to oily water treatment during offshore oil spill clean-up operations is conducted to demonstrate the applicability of the proposed approach. The results suggest that producing a random sequence of triangular fuzzy numbers in a given interval is equivalent to a normal distribution when using the centroid defuzzification method. It also shows that the defuzzified optimal solutions follow the normal distribution and range from 3,000-3,700 tons, given the budget constraint (CAD 110,000-150,000). The normality seems to be able to propagate throughout the optimization process, yet this interesting finding deserves more in-depth study and needs more rigorous mathematical proof to validate its applicability and feasibility. In addition, the optimal decision variables can be categorized into several groups with different probability such that decision makers can wisely allocate limited resources with higher confidence in a short period of time. This study is expected to advise the industries and authorities on how to distribute resources and maximize the treatment efficiency of oily water in a short period of time, particularly in the context of harsh environments

ENV-653: PRODUCTION OF BIOSURFACTANT BY RHODOCOCCUS ERYTHROPOLIS SP. CULTIVATED IN A NOVEL FISH WASTE COMPOST EXTRACT SUBSTRATE

Compost generated through fish waste composting could provide an effective source of nutrient-rich organic matter for microbial growth, leading to the production of valuable products such as biosurfactants. Existing biosurfactant production is a relative expensive process and raw materials contribute about 30% of the production cost. Utilizing waste streams such as fish waste compost (FWC) as a substrate is an economically viable alternative. In this study, biosurfactant was produced by Rhodococcus erythropolis sp. P6-4P, a strain isolated from the North Atlantic Ocean. Biosurfactant production with FWC extract was compared with other soluble and insoluble carbon and nitrogen sources using emulsification assay and surface tension measurement. FWC extract showed good potential as an unconventional source of nutrient for microbial growth. The produced biosurfactant under optimum condition obtained via response surface methodology was further characterized for total carbohydrate, total lipid and total protein content. The results provided evidence for using FWC extract as a novel substrate for biosurfactant production

ENV-624: A NEW HIGH-YIELDING BIO-DISPERSANT PRODUCER MUTATED FROM RHODOCOCCUS ERYTHROPOLIS STRAIN P6-4P

Preeminent effectiveness and feasibility of dispersants have been the key reasons for their widely serving as the response agents in oil spill responses. Moreover, dispersants can also overcome the limitation factors of other countermeasures like accessibility, weather conditions, sea states, and oil thickness. However, the public concerns of the usages of the chemically synthetic dispersants are also essential due to their toxicity and persistency in the ecosystem. Bio-dispersants can be a promising alternative as the proven features of lower toxicity and persistency while with high effectiveness, but its broad application prospects are currently restricted by the high production cost that is 3-10 times more than chemical synthetic ones because of the low productivity. Thus, a hyper bio-dispersant producer will be the desired coping strategy. An isolated bio-dispersant producer from NL offshore, Rhodococcus erythropolis strain P6-4P was selected for generating high-yielding producers by mutation. After UV mutagenesis, 21 enhanced mutants were selected through oil spreading screening method. Further productivity quantify test of critical micelle dilution (CMD) with higher resolution was conducted to these mutants. An outstanding mutant showed CMD as high as 225 while 15.4 is the CMD of the wild type strain, which means the new mutant is 14.6 times increase. The 16S rDNA sequencing results revealed that the 16 S ribosomal DNA of the mutant 100% matched with the original strain indicating the mutation occurred on other parts of the genome which will be identified through next-generation sequencing and comparative analysis in the future study. This mutated high-yielding strain was capable to significantly improve the production rate and the total yield of bio-dispersants. The yield of crude bio-dispersant was 54g per liter with 6 days incubation. At 4mg/uL crude product/crude oil ratio, the dispersion effectiveness was found comparable to Corexit 9500A at 1:25 (dispersant/crude oil ratio). Future works on further mutagenesis base on this new high-producing strain by novel mutation methods were also discussed

Wetland Degradation and Ecological Restoration

Wetlands are among the most important ecosystems on earth and functioned as the “kidneys” of the earth, which play an important role in maintaining ecological service functions. However, with the rapid growth in human populations, wetlands worldwide are suffering from serious degradation or loss as affected by wetland pollution, wetland reclamation, civilization and land use changes, and so forth. Wetland degradation has potential influences on human health, biodiversity, regional climate, and regional ecological security. Therefore, it is an urgent task to recover these degraded wetlands. In recent years, wetland protection, restoration, and its reasonable exploitation have been paid much more attention to by most governments and researchers. Moreover, wetland restoration has become the frontier fields of wetlands science, which has been listed as one of important themes in these recent international wetlands and ecological conferences. Understanding wetland degradation processes can contribute to better effective wetland restoration. Therefore, we organized this special issue on “wetland degradation and ecological restoration.” The objective of this special issue is to emphasize the effects of human activities on wetland ecosystems, the relationships between soil, water, and plant in wetlands, and wetland restoration issues and applications

Primary localized histoplasmosis with lesions restricted to the mouth in a Chinese HIV-negative patient

SummaryHistoplasmosis is a deep mycosis caused by Histoplasma capsulatum, which is endemic in many areas of the world but is relatively rare in China. Although the majority of cases present as a mild to moderate flu-like disease requiring only supportive therapy, approximately 1% of patients experience more serious pulmonary and extrapulmonary disease, which can be life-threatening if diagnosis is delayed or the treatment is not initiated rapidly. Definitive diagnosis is usually made by a combination of culture, detection of the organism in tissues, measurement of antibodies, and detection of antigen. We present the case of a 51-year-old patient who presented with histoplasmosis only, with several ulcerated lesions in the oral cavity and without HIV infection, who did not show any detectable signs and symptoms of systemic disease or extra-oral manifestations. Histopathological analysis indicated a chronic inflammatory process with granulomas with yeast-like organisms. Isolation of H. capsulatum and molecular identification provided the definitive diagnosis. Treatment with oral itraconazole led to remission of the oral lesions. This is the first Chinese case report of localized histoplasmosis with lesions restricted to the mouth in an HIV-negative patient

G protein α subunit suppresses sporangium formation through a serine/threonine protein kinase in Phytophthora sojae

Eukaryotic heterotrimeric guanine nucleotide-binding proteins consist of α, β, and γ subunits, which act as molecular switches to regulate a number of fundamental cellular processes. In the oomycete pathogen Phytophthora sojae, the sole G protein α subunit (Gα; encoded by PsGPA1) has been found to be involved in zoospore mobility and virulence, but how it functions remains unclear. In this study, we show that the Gα subunit PsGPA1 directly interacts with PsYPK1, a serine/threonine protein kinase that consists of an N-terminal region with unknown function and a C-terminal region with a conserved catalytic kinase domain. We generated knockout and knockout-complemented strains of PsYPK1 and found that deletion of PsYPK1 resulted in a pronounced reduction in the production of sporangia and oospores, in mycelial growth on nutrient poor medium, and in virulence. PsYPK1 exhibits a cytoplasmic-nuclear localization pattern that is essential for sporangium formation and virulence of P. sojae. Interestingly, PsGPA1 overexpression was found to prevent nuclear localization of PsYPK1 by exclusively binding to the N-terminal region of PsYPK1, therefore accounting for its negative role in sporangium formation. Our data demonstrate that PsGPA1 negatively regulates sporangium formation by repressing the nuclear localization of its downstream kinase PsYPK1.</p

Engineering two-dimensional metal oxides and chalcogenides for enhanced electro- and photocatalysis

Two-dimensional (2D) metal oxides and chalcogenides (MOs &amp; MCs) have been regarded as a new class of promising electro- and photocatalysts for many important chemical reactions such as hydrogen evolution reaction, CO2 reduction reaction and N2 reduction reaction in virtue of their outstanding physicochemical properties. However, pristine 2D MOs &amp; MCs generally show the relatively poor catalytic performances due to the low electrical conductivity, few active sites and fast charge recombination. Therefore, considerable efforts have been devoted to engineering 2D MOs &amp; MCs by rational structural design and chemical modification to further improve the catalytic activities. Herein, we comprehensively review the recent advances for engineering technologies of 2D MOs &amp; MCs, which are mainly focused on the intercalation, doping, defects creation, facet design and compositing with functional materials. Meanwhile, the relationship between morphological, physicochemical, electronic, and optical properties of 2D MOs &amp; MCs and their electro- and photocatalytic performances is also systematically discussed. Finally, we further give the prospect and challenge of the field and possible future research directions, aiming to inspire more research for achieving high-performance 2D MOs &amp; MCs catalysts in energy storage and conversion fields

Isolation of nitrate-reducing bacteria from an offshore reservoir and the associated biosurfactant production

Biosurfactant producing nitrate-reducing bacteria (NRB) in anaerobic reservoir environments are closely associated with souring (H2S) control in the offshore oil and gas industry. Five NRB strains were screened from offshore produced water samples and all were identified as Pseudomonas stutzeri. Their biosurfactant producing abilities when fed on either glucose or glycerol media were investigated. P. stutzeri CX3 reduced the medium surface tension to 33.5 and 29.6 mN m−1, respectively, while growing on glucose or glycerol media. The CX3 strain was further inoculated to examine its growth performance, resulting in 32.4% and 94.5% of nitrate consumption over 228 hours of monitoring in two media, respectively. The composition analysis of the biosurfactant product generated by P. stutzeri CX3 was conducted through thin-layer chromatography, gas chromatography with a flame ionization detector (FID) and Fourier transform infrared spectroscopy (FT-IR). The biosurfactant product was identified as a mixture of a small part of lipopeptides and a large part of glycolipids while its critical micellar concentration (CMC) was as low as 35 mg L−1. The biosurfactant product demonstrated high stability over a wide range of temperature (4–121 °C), pH (2–10), and salinity (0–20% w/v) concentration. The results provided valuable technical and methodological support for effective offshore reservoir souring control and associated enhanced oil recovery activities

Fish Waste Based Lipopeptide Production and the Potential Application as a Bio-Dispersant for Oil Spill Control

There is a growing acceptance worldwide for the application of dispersants as a marine oil spill response strategy. The development of more effective dispersants with less toxicity and higher biodegradability would be a step forward in improving public acceptance and regulatory approvals for their use. By applying advances in environmental biotechnology, a bio-dispersant agent with a lipopeptide biosurfactant produced by Bacillus subtilis N3-1P as the key component was formulated in this study. The economic feasibility of producing biosurfactant (a high-added-value bioproduct) from fish waste-based peptone as a nutrient substrate was evaluated. Protein hydrolyzate was prepared from cod liver and head wastes obtained from fish processing facilities. Hydrolysis conditions (i.e., time, temperature, pH and enzyme to substrate level) for preparing protein hydrolyzates were optimized by response surface methodology using a factorial design. The critical micelle dilution (CMD) value for biosurfactant produced from the fish liver and head waste generated peptones was 54.72 and 47.59 CMD, respectively. Biosurfactant product generated by fish liver peptone had a low critical micelle concentration of 0.18 g L–1 and could reduce the surface tension of distilled water to 27.9 mN/m. Structure characterization proved that the generated biosurfactant product belongs to the lipopeptide class. An alternative to the key surfactant dioctyl sulfosuccinate sodium (DOSS) used in Corexit 9500 has been proposed based on a binary mixture of lipopeptides and DOSS that exhibited synergistic effects. Using the standard baffled flask test, a high dispersion efficiency of 76.8% for Alaska North Slope oil was achieved at a biodispersant composition of 80/20 (v/v) of lipopeptides/DOSS. The results show that fish waste can be utilized to produce a more effective, environmentally acceptable and cost-efficient biodispersant that can be applied to oil spills in the marine environment

UV Stimulated Manganese Dioxide for the Persulfate Catalytic Degradation of Bisphenol A

One of the most commonly produced industrial chemicals worldwide, bisphenol A (BPA), is used as a precursor in plastics, resins, paints, and many other materials. It has been proved that BPA can cause long-term adverse effects on ecosystems and human health due to its toxicity as an endocrine disruptor. In this study, we developed an integrated MnO2/UV/persulfate (PS) process for use in BPA photocatalytic degradation from water and examined the reaction mechanisms, degradation pathways, and toxicity reduction. Comparative tests using MnO2, PS, UV, UV/MnO2, MnO2/PS, and UV/PS processes were conducted under the same conditions to investigate the mechanism of BPA catalytic degradation by the proposed MnO2/UV/PS process. The best performance was observed in the MnO2/UV/PS process in which BPA was completely removed in 30 min with a reduction rate of over 90% for total organic carbon after 2 h. This process also showed a stable removal efficiency with a large variation of pH levels (3.6 to 10.0). Kinetic analysis suggested that 1O2 and SO4