583 research outputs found

    \u3ci\u3eAcrobasis\u3c/i\u3e Shoot Moth (Lepidoptera: Pyralidae) Infestation-Tree Height Link in a Young Black Walnut Plantation

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    Acrobasis shoot moth infestations were evaluated in a young black walnut progeny test for 4 years, from ages 3 to 6. Infestation levels were greatest on the largest trees in the fourth and fifth year after plantation establishment, and were declining by the sixth year. Acrobasis infestation appears to be a problem primarily on young trees less than 2.5 m in height. There was no evidence for genetic resistance to Acrobasis infestation in black walnut

    Regulation of synaptic connectivity: levels of fasciclin II influence synaptic growth in the Drosophila CNS

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    Much of our understanding of synaptogenesis comes from studies that deal with the development of the neuromuscular junction (NMJ). Although well studied, it is not clear how far the NMJ represents an adequate model for the formation of synapses within the CNS. Here we investigate the role of Fasciclin II (Fas II) in the development of synapses between identified motor neurons and cholinergic interneurons in the CNS of Drosophila. Fas II is a neural cell adhesion molecule homolog that is involved in both target selection and synaptic plasticity at the NMJ in Drosophila. In this study, we show that levels of Fas II are critical determinants of synapse formation and growth in the CNS. The initial establishment of synaptic contacts between these identified neurons is seemingly independent of Fas II. The subsequent proliferation of these synaptic connections that occurs postembryonically is, in contrast, significantly retarded by the absence of Fas II. Although the initial formation of synaptic connectivity between these neurons is seemingly independent of Fas II, we show that their formation is, nevertheless, significantly affected by manipulations that alter the relative balance of Fas II in the presynaptic and postsynaptic neurons. Increasing expression of Fas II in either the presynaptic or postsynaptic neurons, during embryogenesis, is sufficient to disrupt the normal level of synaptic connectivity that occurs between these neurons. This effect of Fas II is isoform specific and, moreover, phenocopies the disruption to synaptic connectivity observed previously after tetanus toxin light chain-dependent blockade of evoked synaptic vesicle release in these neurons

    A tail-like assembly at the portal vertex in intact herpes simplex type-1 virions

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    Herpes viruses are prevalent and well characterized human pathogens. Despite extensive study, much remains to be learned about the structure of the genome packaging and release machinery in the capsids of these large and complex double-stranded DNA viruses. However, such machinery is well characterized in tailed bacteriophage, which share a common evolutionary origin with herpesvirus. In tailed bacteriophage, the genome exits from the virus particle through a portal and is transferred into the host cell by a complex apparatus (i.e. the tail) located at the portal vertex. Here we use electron cryo-tomography of human herpes simplex type-1 (HSV-1) virions to reveal a previously unsuspected feature at the portal vertex, which extends across the HSV-1 tegument layer to form a connection between the capsid and the viral membrane. The location of this assembly suggests that it plays a role in genome release into the nucleus and is also important for virion architecture

    Understanding the independent dancer: roles, development and success

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    Little research has been published about the varied role of the independent dancer. The aim of this study was to provide insight into the work independent dancers undertake and how their careers change over time. Semi-structured interviews were conducted with 14 independent dancers. Content analysis revealed that the dancers had multifaceted careers that relied on both formal and informal activities, and varied according to three distinct stages (early, middle, late). The experiences reported by the dancers indicated that the realities of the independent dancer's role are not sufficiently recognised or supported within the industry

    The age of the directly imaged planet host star k Andromedae determined from interferometric observations

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    κ Andromedae, an early-type star that hosts a directly imaged low-mass companion, is expected to be oblate due to its rapid rotational velocity (v sin i = ~162 km s⁻¹). We observed the star with the CHARA Array's optical beam combiner, PAVO, measuring its size at multiple orientations and determining its oblateness. The interferometric measurements, combined with photometry and this v sin i value are used to constrain an oblate star model that yields the fundamental properties of the star and finds a rotation speed that is ~85% of the critical rate and a low inclination of ~30°. Three modeled properties (the average radius, bolometric luminosity, and equatorial velocity) are compared to MESA evolution models to determine an age and mass for the star. In doing so, we determine an age for the system of 47_₄₀⁺²⁷ Myr. Based on this age and previous measurements of the companion's temperature, the BHAC15 evolution models imply a mass for the companion of 22_₉⁺⁸ MJ.This work is based upon observations obtained with the Georgia State University Center for High Angular Resolution Astronomy Array at Mount Wilson Observatory. The CHARA Array is supported by the National Science Foundation under grants AST-1211929 and AST- 1411654. Institutional support has been provided from the GSU College of Arts and Sciences and the GSU Office of the Vice President for Research and Economic Development. J.J. and R.J.W. acknowledge support from NSF AAG grants 1009643 and 1517762

    Grid-Based Atmospheric Retrievals for Reflected-Light Spectra of Exoplanets using PSGnest

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    Techniques to retrieve the atmospheric properties of exoplanets via direct observation of their reflected light have often been limited in scope due to computational constraints imposed by the forward-model calculations. We have developed a new set of techniques which significantly decreases the time required to perform a retrieval while maintaining accurate results. We constructed a grid of 1.4 million pre-computed geometric albedo spectra valued at discrete sets of parameter points. Spectra from this grid are used to produce models for a fast and efficient nested sampling routine called PSGnest. Beyond the upfront time to construct a spectral grid, the amount of time to complete a full retrieval using PSGnest is on the order of seconds to minutes using a personal computer. An extensive evaluation of the error induced from interpolating intermediate spectra from the grid indicates that this bias is insignificant compared to other retrieval error sources, with an average coefficient of determination between interpolated and true spectra of 0.998. We apply these new retrieval techniques to help constrain the optimal bandpass centers for retrieving various atmospheric and bulk parameters from a LuvEx-type mission observing several planetary archetypes. We show that spectral observations made using a 20\% bandpass centered at 0.73 microns can be used alongside our new techniques to make detections of H2OH_2O and O2O_2 without the need to increase observing time beyond what is necessary for a signal-to-noise ratio of 10. The methods introduced here will enable robust studies of the capabilities of future observatories to characterize exoplanets.Comment: 32 pages, 17 figures. Accepted for publication in The Astronomical Journa

    The microbiome of the marine flatworm Macrostomum lignano provides fitness advantages and exhibits circadian rhythmicity

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    AbstractThe close association between animals and their associated microbiota is usually beneficial for both partners. Here, we used a simple marine model invertebrate, the flatworm Macrostomum lignano, to characterize the host-microbiota interaction in detail. This analysis revealed that the different developmental stages each harbor a specific microbiota. Studies with gnotobiotic animals clarified the physiological significance of the microbiota. While no fitness benefits were mediated by the microbiota when food was freely available, animals with microbiota showed significantly increased fitness with a reduced food supply. The microbiota of M. lignano shows circadian rhythmicity, affecting both the total bacterial load and the behavior of specific taxa. Moreover, the presence of the worm influences the composition of the bacterial consortia in the environment. In summary, the Macrostomum-microbiota system described here can serve as a general model for host-microbe interactions in marine invertebrates.</jats:p
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