Effect of Culture, Age and Pruning on Mass Production of the VA Mycorrhizal Fungus, Glomus fascicuiatum.

Glomus fasciculatum .pot cultures were raised in Rhodes grass (Chloris gayana Kunth) using perlite - soilrite mix (1:1 by volume) in 16 cm pots holding 700 g substrate and were harvested at different intervals. Cultures harvested on the 75th day after sowing had the maximum number of infective propagules. In a second experiment, pruning was found to reduce the mychizal propagules up to 4 weeks. However, the propagules increased from 5th week onwards

Microbial Consortium Promotes Growth of Zinnia and Balsam Seedlings Raised in pro trays

Zinnia and Balsam are flowering plants with high economic importance in floriculture. Inoculation of the planting medium with a beneficial microbial consortium is an innovative approach to produce quality and healthy seedlings in floriculture. In the present study the influence of a microbial consortium of the arbuscular mycorrhizal fungus (AMF) Funneliformis mosseae and a plant growth promoting rhizobacterium (PGPR) Bacillus sonorensis on flowering plants Zinnia and Balsam in pro-trays under poly house conditions was investigated. Estimation of various plant growth parameters such as plant height, stem diameter, bio-volume index, vigour index, plant strength, fresh weight, dry weight and nutrient uptake was carried out to analyse the ability of the consortium to improve seedling growth. Microbial parameters such as mycorrhizal root colonization and spore count, and population of PGPR in substrate was also studied. The results suggested that inoculating the substrate in pro trays before sowing the seeds with the consortium increased plant growth significantly compared to the uninoculated plants

Response of cardamom (Elettaria cardamomum Maton) seedlings to vesicular arbuscular mycorrhizal fungi

The growth response of cardamom (Elettaria cardamomum) seedlings (cv, Malabar) to 13 different vesicular arbuscular mycorrhizal fungi was tested under mat house conditions at Bangalore, India, In general, seedlings inoulated with the fungi grew taller, had more number of leaves and tillers, increased seedling biomass and uptake of nutrients compared to control seedlings, Among the various mycorrhizal fungi tested, seedlings inoculated with Gigaspora margarita and Glomus monosporum exhibited significantly higher growth with increased uptake of nutrients, &nbsp

Response of cardamom (Elettaria cardamomum Maton) seedlings to vesicular arbuscular mycorrhizal fungi

The growth response of cardamom (Elettaria cardamomum) seedlings (cv, Malabar) to 13 different vesicular arbuscular mycorrhizal fungi was tested under mat house conditions at Bangalore, India, In general, seedlings inoulated with the fungi grew taller, had more number of leaves and tillers, increased seedling biomass and uptake of nutrients compared to control seedlings, Among the various mycorrhizal fungi tested, seedlings inoculated with Gigaspora margarita and Glomus monosporum exhibited significantly higher growth with increased uptake of nutrients, &nbsp

Brain wave classification for divergent hand movements

Brain-Computer Interface (BCI) is an emerging technology in medical diagnosis and rehabilitation. In this study, by the acquisition of Electroencephalogram (EEG) signals from 30 healthy participants who perform four different hand movements, necessary features are extracted and classified to determine their accuracies. Statistical time domain features are extracted from the mu and beta frequency band. The Event related desynchronization (ERD)/Event related synchronization (ERS) measurements are extracted, from which it was evident that both mu and beta frequency bands are more efficient in the C3 channel. By applying the Paired Samples t-test, the extracted features are analyzed and were determined to have a 95% significant level of difference between the mu and beta band, being statistically efficient in the beta band of the C3 channel. By employing different classifiers such as Support Vector Machine (SVM), Linear Discriminant Analysis (LDA), Quadratic Discriminant Analysis (QDA), Naïve Bayesian classifier and Binary Decision Tree (BDT) algorithms on both channel’s mu and beta frequency bands, it was observed that the performance of beta frequency band classifiers shows 90% accuracy in binary class classification. In the comparative study of all these classifiers, LDA and Naïve Bayes show above 95% accuracy for binary class classification

Interaction between a vesicular-arbuscular mycorrhizal fungus and Streptomyces cinnamomeous and their effects on finger millet

Growth and P nutrition of Eleusine coracana on a sterile P-deficient soil was improved by inoculation with either Glomus fasciculatus or S. cinnamomeous. The micro-organisms interacted antagonistically when added simultaneously or with a 2-wk interval between them: S. reduced spore production and development of infection by G. while G. reduced the multiplication of S. Because of this antagonism dual inoculations stimulated plant growth less than single ones.ADDITIONAL ABSTRACT:The growth and phosphorus nutrition of Eleusine coracana on a sterile P-deficient soil was improved by inoculation with the vesicular-arbuscular mycorrhizal fungus Glomus fasciculatus or with Streptomyces cinnamomeous. These microorganisms interacted antagonistically, so inoculation of E. coracana with both did not give as much growth stimulation as inoculation with either alon

Response of Coleus aromaticus to Glomus fasciculatum and other beneficial soil microflora

Stem cuttings of Coleus aromaticus were treated with vesicular arbuscular mycorrhiza (Glomus fasciculatum) and beneficial microorganisms viz., Bacillus coagulans, Pseudomonas fluorescens, Azotobacter chroococcum and Trichoderma harzianum either singly or in combination of organisms. The number of leaves and branches; total biomass, nitrogen and phosphorus contents were higher in plants treated with G. fasciculatum alone. Synergistic effect was noticed especially in plants treated with G. fasciculatum + P. fluorescens. Mycorrhizal colonization in roots was also maximum when V AM was applied alone. &nbsp

Brain wave classification for divergent hand movements

765-773Brain-Computer Interface (BCI) is an emerging technology in medical diagnosis and rehabilitation. In this study, by the acquisition of Electroencephalogram (EEG) signals from 30 healthy participants who perform four different hand movements, necessary features are extracted and classified to determine their accuracies. Statistical time domain features are extracted from the mu and beta frequency band. The Event related desynchronization (ERD)/Event related synchronization (ERS) measurements are extracted, from which it was evident that both mu and beta frequency bands are more efficient in the C3 channel. By applying the Paired Samples t-test, the extracted features are analyzed and were determined to have a 95% significant level of difference between the mu and beta band, being statistically efficient in the beta band of the C3 channel. By employing different classifiers such as Support Vector Machine (SVM), Linear Discriminant Analysis (LDA), Quadratic Discriminant Analysis (QDA), Naïve Bayesian classifier and Binary Decision Tree (BDT) algorithms on both channel’s mu and beta frequency bands, it was observed that the performance of beta frequency band classifiers shows 90% accuracy in binary class classification. In the comparative study of all these classifiers, LDA and Naïve Bayes show above 95% accuracy for binary class classification

Response of Coleus aromaticus to Glomus fasciculatum and other beneficial soil microflora

Stem cuttings of Coleus aromaticus were treated with vesicular arbuscular mycorrhiza (Glomus fasciculatum) and beneficial microorganisms viz., Bacillus coagulans, Pseudomonas fluorescens, Azotobacter chroococcum and Trichoderma harzianum either singly or in combination of organisms. The number of leaves and branches; total biomass, nitrogen and phosphorus contents were higher in plants treated with G. fasciculatum alone. Synergistic effect was noticed especially in plants treated with G. fasciculatum + P. fluorescens. Mycorrhizal colonization in roots was also maximum when V AM was applied alone. &nbsp

Preparation and Characterization of Silver Nanoparticle/Aloe Vera Incorporated PCL/PEO matrix for wound dressing application

Polymeric wound dressing materials have remarkable mechanical, structural, and biocompatible behavior. In this work, a polymer matrix of Polycaprolactone (PCL)/Polyethylene Oxide (PEO) incorporated with Aloe Vera (AV) extract and silver nanoparticles were prepared for wound dressing application. Initially, the phytochemicals from AV were extracted by Soxhlet apparatus, and then the aloe extract was used as a reducing agent to synthesize silver nanoparticles (Ag NP). Ag NP's formation was confirmed by the presence of a characteristic UV absorbance peak at 420 nm. Ag NP's average diameter and shape were found to be between 10-50 nm and spherical, respectively. AV extract and Ag NP were incorporated into PCL/PEO polymer solution to prepare the polymer matrix by solution casting method. Box-Behnken design (BBD) was used to study the effect of Ag NP concentration, AV extract percentage, and PEO weight percentage concerning PCL on wound dressing application. Water Vapor Transmission Rate (WVTR) and swelling properties of all the sample were tested and found that the PEO and AV extract plays a major role in both swelling and WVTR irrespective of Ag NP concentration. The antimicrobial property of synthesized Ag NP was studied against gram-negative bacteria Escherichia coli with control samples (PCL and PCL/PEO), Ag NP with 150 mg concentration showed a higher zone of inhibition than the other concentrations. Thus, the prepared PCL/PEO polymer matrix incorporated with AV extract and Ag NP can be used as an effective wound dressing material