Synthesis of 4-Thiazolidinone Small Molecules as Potential Inhibitors of the Arp2/3 Complex

Life-essential cellular processes such as endocytosis, motility, and division rely on a cell’s ability to precisely regulate construction of actin filaments in response to external factors and signals. Intrinsically involved in this process is the Actin Related Protein 2/3 (Arp2/3) Complex, a seven-subunit ATPase that functions by nucleating a daughter branch of actin from the side of a pre-existing microfilament. Active Arp2/3 complex is necessary for the proliferation of certain metastatic cancers, and inhibition of Arp2/3 complex is emerging as a potentially useful treatment strategy for such cancers. We describe synthesis and in vitro assays of 4-thiazolidinones predicted by computational methods to inhibit of Arp2/3 Complex strongly, and therefore serve as potential lead compounds for drug development. Known Arp2/3 inhibitor CK-869 serves as the starting point for derivative synthesis. We discuss the efforts towards the synthesis of new compounds and the biochemical data collected about their potency

Synthesis of Small Molecule Derivatives of CK-666 as Potential Inhibitors of the Arp2/3 Complex

The Actin-Related Protein (Arp) 2/3 Complex is an actin nucleating factor intrinsically involved in cellular regulation of actin networks during life-essential processes such as motility. Overexpression of the Arp2/3 complex has indicated as a factor allowing the proliferation of certain metastatic cancers. This work describes the synthesis and in vitro biochemical testing of several molecules predicted by computational docking to be inhibitors of Arp2/3 Complex, and therefore of potential interest in clinical applications. The molecules are designed based off of the structure of known Arp2/3 inhibitor CK-666, which was discovered via high-throughput screening. Details of the synthesis of the tryptamine derivatives are discussed, and the bulk actin polymerization assay used to determine potency of the new compounds is discussed

Classification of Generalized Multiresolution Analyses

We discuss how generalized multiresolution analyses (GMRAs), both classical and those defined on abstract Hilbert spaces, can be classified by their multiplicity functions $m$ and matrix-valued filter functions $H$. Given a natural number valued function $m$ and a system of functions encoded in a matrix $H$ satisfying certain conditions, a construction procedure is described that produces an abstract GMRA with multiplicity function $m$ and filter system $H$. An equivalence relation on GMRAs is defined and described in terms of their associated pairs $(m,H)$. This classification system is applied to classical examples in $L^2 (\mathbb R^d)$ as well as to previously studied abstract examples.Comment: 18 pages including bibliograp

A Concept Fully Realized: The Beauty of Developing an Internally-Housed Wiki Server

Librarians at Louisiana State University Health Sciences Center Shreveport recently disposed of their old wiki in favor of a customizable, internally-housed wiki server. The initial catalyst for change was simply a need for more space. Gradually, the wiki’s scope and purpose transformed, making it into a resource that is relevant to the work of all departments within the library. The new wiki serves as a fully collaborative workspace and simultaneously supports the mission of institutional knowledge management, features that were largely ignored or neglected in the previous incarnation. This poster session will document the process leading to the wiki server’s creation and implementation. It will also highlight the improved functionality granted by the new wiki

Cohomology of Polynomials Under an Irrational Rotation

A new description of cohomology of functions under an irrational rotation is given in terms of symmetry properties of the functions on subintervals of [0, 1]. This description yields a method for passing information about the cohomology classes for a given irrational to the cohomology classes for an equivalent irrational

On Functions That Are Trivial Cocycles for a Set of Irrationals. II

Two results are obtained about the topological size of the set of irrationals for which a given function is a trivial cocycle. An example of a continuous function which is a coboundary with non-L(1) cobounding function is constructed