The association between parenting behavior and somatization in adolescents explained by physiological responses in adolescents

Introduction: This study adds to the knowledge on somatization in adolescents by exploring its relation with parenting behavior and the mediating/moderating role of physiological responses in adolescents to parenting behavior. Method: Eighteen adolescents with high and 18 adolescents with low somatization scores and their mothers completed a discussion task, from which observed parenting behavior scores were derived. Skin conductance in adolescents was measured before and during the discussion. Results: For adolescents with high levels of physiological responses, unadaptive parenting was related to a higher chance of high somatization scores. For low physiologically responsive adolescents, the relation between parenting behavior and somatization was not significant. Conclusion: Parenting behavior is not univocally related to somatization in adolescents, but the association depends on physiological responses in adolescents. (C) 2014 Elsevier B.V. All rights reserved

Experimental study of factors influencing the risk of drift from field sprayers Part 2: Spray application technique.

Recently, spray drift and its effects have become an important aspect of risk assessment in the registration process of pesticides in Belgium. In this regulation, drift reducing spray application techniques can be used to reduce buffer zones. The purpose of this research is to measure and compare the amount of drift sediment for different spray application techniques under field conditions. A drift prediction equation for the reference spraying was used to compare other spraying techniques with the reference spraying, under different weather conditions. Drift measurements were performed for several combinations of nozzle type (flat fan, low-drift, air injection) and size (ISO 02, 03, 04 and 06), spray pressure (2, 3 and 4 bar), driving speed (4, 6, 8 and 10 km.h-1) and spray boom height (0.3, 0.5 and 0.75 m). Nozzle type as well as spray pressure, driving speed and spray boom height, have an important effect on the amount of spray drift. Larger nozzle sizes, lower spray pressures and driving speeds and lower spray boom heights generally reduce spray drift. Concerning nozzle types, air injection nozzles have the highest drift reduction potential followed by the low-drift nozzles and the standard flat fan nozzles

The impact of resource dependence of the mechanisms of life on the spatial population dynamics of an in silico microbial community

Biodiversity has a critical impact on ecosystem functionality and stability, and thus the current biodiversity crisis has motivated many studies of the mechanisms that sustain biodiversity, a notable example being non-transitive or cyclic competition. We therefore extend existing microscopic models of communities with cyclic competition by incorporating resource dependence in demographic processes, characteristics of natural systems often oversimplified or overlooked by modellers. The spatially explicit nature of our individual-based model of three interacting species results in the formation of stable spatial structures, which have significant effects on community functioning, in agreement with experimental observations of pattern formation in microbial communities. Published by AIP Publishing

Experimental study of factors influencing the risk of drift from field sprayers, Part 1: Meteorological conditions

Spray drift can be defined as the quantity of plant protection product that is carried out of the sprayed (treated) area by the action of air currents during the application process. This continues to be a major problem in applying agricultural pesticides. The purpose of this research is to measure and compare the amount of drift for different climatological conditions under field conditions. Sedimenting spray drift was determined by sampling in a defined downwind area at different positions in a flat meadow using horizontal drift collectors for a reference spraying. Meteorological conditions were monitored during each experiment. A drift prediction equation for the reference spraying was set up to predict the expected magnitude of sedimenting at various drift distances and atmospheric conditions. The measurements proved the important effect of weather conditions (temperature, relative humidity and wind speed) on the amount of spray drift. A lower wind speed or a higher relative humidity decreases the amount of spray drift. Taking into account the correlation between temperature and relative humidity, a lower temperature will also result in lower drift values due to the cumulative effect of relative humidity. This equation can be used to quantify the effect of meteorological conditions, to compare measurements using other spraying techniques under different weather conditions to the reference spraying and to perform spray drift risk assessments

Forensic child and Adolescent Psychiatry and mental health in Europe

Background When faced with the discovery of their child’s self-harm, mothers and fathers may re-evaluate their parenting strategies. This can include changes to the amount of support they provide their child and changes to the degree to which they control and monitor their child. Methods We conducted an in-depth qualitative study with 37 parents of young people who had self-harmed in which we explored how and why their parenting changed after the discovery of self-harm. Results Early on, parents often found themselves “walking on eggshells” so as not to upset their child, but later they felt more able to take some control. Parents’ reactions to the self-harm often depended on how they conceptualised it: as part of adolescence, as a mental health issue or as “naughty behaviour”. Parenting of other children in the family could also be affected, with parents worrying about less of their time being available for siblings. Many parents developed specific strategies they felt helped them to be more effective parents, such as learning to avoid blaming themselves or their child for the self-harm and developing new ways to communicate with their child. Parents were generally eager to pass their knowledge on to other people in the same situation. Conclusions Parents reported changes in their parenting behaviours after the discovery of a child’s self-harm. Professionals involved in the care of young people who self-harm might use this information in supporting and advising parents.</p

Practical Tools to Implement Massive Parallel Pyrosequencing of PCR Products in Next Generation Molecular Diagnostics

Despite improvements in terms of sequence quality and price per basepair, Sanger sequencing remains restricted to screening of individual disease genes. The development of massively parallel sequencing (MPS) technologies heralded an era in which molecular diagnostics for multigenic disorders becomes reality. Here, we outline different PCR amplification based strategies for the screening of a multitude of genes in a patient cohort. We performed a thorough evaluation in terms of set-up, coverage and sequencing variants on the data of 10 GS-FLX experiments (over 200 patients). Crucially, we determined the actual coverage that is required for reliable diagnostic results using MPS, and provide a tool to calculate the number of patients that can be screened in a single run. Finally, we provide an overview of factors contributing to false negative or false positive mutation calls and suggest ways to maximize sensitivity and specificity, both important in a routine setting. By describing practical strategies for screening of multigenic disorders in a multitude of samples and providing answers to questions about minimum required coverage, the number of patients that can be screened in a single run and the factors that may affect sensitivity and specificity we hope to facilitate the implementation of MPS technology in molecular diagnostics