5,848 research outputs found

    Immunocytochemical localization of casein kinase II during interphase and mitosis

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    We have developed specific antibodies to synthetic peptide antigens that react with the individual subunits of casein kinase II (CKII). Using these antibodies, we studied the localization of CKII in asynchronous HeLa cells by immunofluorescence and immunoelectron microscopy. Further studies were done on HeLa cells arrested at the G1/S transition by hydroxyurea treatment. Our results indicate that the CKII alpha and beta subunits are localized in the cytoplasm during interphase and are distributed throughout the cell during mitosis. Further electron microscopic investigation revealed that CKII alpha subunit is associated with spindle fibers during metaphase and anaphase. In contrast, the CKII alpha' subunit is localized in the nucleus during G1 and in the cytoplasm during S. Taken together, our results suggest that CKII may play significant roles in cell division control by shifting its localization between the cytoplasm and nucleus

    Room temperature spin coherence in ZnO

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    Time-resolved optical techniques are used to explore electron spin dynamics in bulk and epilayer samples of n-type ZnO as a function of temperature and magnetic field. The bulk sample yields a spin coherence time T2* of 20 ns at T = 30 K. Epilayer samples, grown by pulsed laser deposition, show a maximum T2* of 2 ns at T = 10 K, with spin precession persisting up to T = 280 K.Comment: 3 pages, 3 figure

    Electron affinity of Li: A state-selective measurement

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    We have investigated the threshold of photodetachment of Li^- leading to the formation of the residual Li atom in the 2p2P2p ^2P state. The excited residual atom was selectively photoionized via an intermediate Rydberg state and the resulting Li^+ ion was detected. A collinear laser-ion beam geometry enabled both high resolution and sensitivity to be attained. We have demonstrated the potential of this state selective photodetachment spectroscopic method by improving the accuracy of Li electron affinity measurements an order of magnitude. From a fit to the Wigner law in the threshold region, we obtained a Li electron affinity of 0.618 049(20) eV.Comment: 5 pages,6 figures,22 reference

    Design and construction of a remote monitoring and control system for a dehumidifier combined with a heating module

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    Background: Protected cultivation (e.g., greenhouse) is spreading mainly for high-income crops such as paprika, strawberry, and tomato. The temperature and the humidity are especially important during cultivation. A dehumidifier combined with a heating module is under development. In the study, a remote monitoring and control system for the prototype was designed and constructed. Methods: The dehumidifier prototype was composed of a dehumidifier, a fan blower, and heating modules. The remote monitoring and control system was composed of temperature-humidity measuring sensor network, a central processing module, a dehumidifier network, and smartphone application. First, the sensing network was tested for different communication distances and number of data variables. Second, remote monitoring and control performance was tested using the smartphone application by signal intensity and time of the day. Results and discussion: Monitoring performance was favourable within 15 m distance with the tested ZigBee module, and a wireless communication network was established considering the communication distance and greenhouse length (i.e., 100 m). Remote monitoring and control was successful for all of the tested signal intensity and time of the day, although the data transmission speed was affected significantly. Components of the dehumidifier were successfully controlled within 30 s. Conclusion: The remote monitoring and control system was tested only under experimental conditions. For improved performance and practical application, the prototype needs to be further tested under crop growing conditions, and the control algorithm needs to be also improved

    Antimony-doped graphene nanoplatelets

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    Heteroatom doping into the graphitic frameworks have been intensively studied for the development of metal-free electrocatalysts. However, the choice of heteroatoms is limited to non-metallic elements and heteroatom-doped graphitic materials do not satisfy commercial demands in terms of cost and stability. Here we realize doping semimetal antimony (Sb) at the edges of graphene nanoplatelets (GnPs) via a simple mechanochemical reaction between pristine graphite and solid Sb. The covalent bonding of the metalloid Sb with the graphitic carbon is visualized using atomic-resolution transmission electron microscopy. The Sb-doped GnPs display zero loss of electrocatalytic activity for oxygen reduction reaction even after 100,000 cycles. Density functional theory calculations indicate that the multiple oxidation states (Sb3+ and Sb5+) of Sb are responsible for the unusual electrochemical stability. Sb-doped GnPs may provide new insights and practical methods for designing stable carbon-based electrocatalystsclose0

    Key distillation from quantum channels using two-way communication protocols

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    We provide a general formalism to characterize the cryptographic properties of quantum channels in the realistic scenario where the two honest parties employ prepare and measure protocols and the known two-way communication reconciliation techniques. We obtain a necessary and sufficient condition to distill a secret key using this type of schemes for Pauli qubit channels and generalized Pauli channels in higher dimension. Our results can be applied to standard protocols such as BB84 or six-state, giving a critical error rate of 20% and 27.6%, respectively. We explore several possibilities to enlarge these bounds, without any improvement. These results suggest that there may exist weakly entangling channels useless for key distribution using prepare and measure schemes.Comment: 21 page

    Mixed Bino-Wino-Higgsino Dark Matter in Gauge Messenger Models

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    Almost degenerate bino and wino masses at the weak scale is one of unique features of gauge messenger models. The lightest neutralino is a mixture of bino, wino and higgsino and can produce the correct amount of the dark matter density if it is the lightest supersymmetric particle. Furthermore, as a result of squeezed spectrum of superpartners which is typical for gauge messenger models, various co-annihilation and resonance regions overlap and very often the correct amount of the neutralino relic density is generated as an interplay of several processes. This feature makes the explanation of the observed amount of the dark matter density much less sensitive to fundamental parameters. We calculate the neutralino relic density assuming thermal history and present both spin independent and spin dependent cross sections for the direct detection. We also discuss phenomenological constraints from b to s gamma and muon g-2 and compare results of gauge messenger models to well known results of the mSUGRA scenario.Comment: 27 pages, 9 figures, references added, version to appear at JCA

    Singular Cucker-Smale Dynamics

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    The existing state of the art for singular models of flocking is overviewed, starting from microscopic model of Cucker and Smale with singular communication weight, through its mesoscopic mean-filed limit, up to the corresponding macroscopic regime. For the microscopic Cucker-Smale (CS) model, the collision-avoidance phenomenon is discussed, also in the presence of bonding forces and the decentralized control. For the kinetic mean-field model, the existence of global-in-time measure-valued solutions, with a special emphasis on a weak atomic uniqueness of solutions is sketched. Ultimately, for the macroscopic singular model, the summary of the existence results for the Euler-type alignment system is provided, including existence of strong solutions on one-dimensional torus, and the extension of this result to higher dimensions upon restriction on the smallness of initial data. Additionally, the pressureless Navier-Stokes-type system corresponding to particular choice of alignment kernel is presented, and compared - analytically and numerically - to the porous medium equation

    Graduate HRD Programs in South Korea

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    The Problem To address national issues associated with societal development, many countries are recognizing the need to educate individuals who will lead developmental initiatives. While many previous studies have been conducted to examine how and what content of human resource development (HRD) has been taught in Western countries, similar research efforts have been rarely conducted in Asian countries such as South Korea. The lack of comparative study creates a research gap in understanding how HRD programs have been designed and implemented in different countries.Yeshttps://us.sagepub.com/en-us/nam/manuscript-submission-guideline
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