13 research outputs found

    Separase is required for chromosome segregation during meiosis I in Caenorhabditis elegans

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    AbstractBackground: Chromosome segregation during mitosis and meiosis is triggered by dissolution of sister chromatid cohesion, which is mediated by the cohesin complex. Mitotic sister chromatid disjunction requires that cohesion be lost along the entire length of chromosomes, whereas homolog segregation at meiosis I only requires loss of cohesion along chromosome arms. During animal cell mitosis, cohesin is lost in two steps. A nonproteolytic mechanism removes cohesin along chromosome arms during prophase, while the proteolytic cleavage of cohesin's Scc1 subunit by separase removes centromeric cohesin at anaphase. In Saccharomyces cerevisiae and Caenorhabditis elegans, meiotic sister chromatid cohesion is mediated by Rec8, a meiosis-specific variant of cohesin's Scc1 subunit. Homolog segregation in S. cerevisiae is triggered by separase-mediated cleavage of Rec8 along chromosome arms. In principle, chiasmata could be resolved proteolytically by separase or nonproteolytically using a mechanism similar to the mitotic “prophase pathway.”Results: Inactivation of separase in C. elegans has little or no effect on homolog alignment on the meiosis I spindle but prevents their timely disjunction. It also interferes with chromatid separation during subsequent embryonic mitotic divisions but does not directly affect cytokinesis. Surprisingly, separase inactivation also causes osmosensitive embryos, possibly due to a defect in the extraembryonic structures, referred to as the “eggshell.”Conclusions: Separase is essential for homologous chromosome disjunction during meiosis I. Proteolytic cleavage, presumably of Rec8, might be a common trigger for the first meiotic division in eukaryotic cells. Cleavage of proteins other than REC-8 might be necessary to render the eggshell impermeable to solutes

    Similarities and differences in the gene expression profiles of MnSOD over-expressing and aging in

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    <p><b>Copyright information:</b></p><p>Taken from "Transcriptional profiling of MnSOD-mediated lifespan extension in reveals a species-general network of aging and metabolic genes"</p><p>http://genomebiology.com/2007/8/12/R262</p><p>Genome Biology 2007;8(12):R262-R262.</p><p>Published online 9 Dec 2007</p><p>PMCID:PMC2246264.</p><p></p> Diagram of sampling points for the transgenic and control flies used in the gene expression profiling studies. For the control, treated (+DOX) and untreated (-DOX) flies were sampled at the 50% survival of the untreated sample, which was also approximately the 50% survival point of the treated flies. For the transgenic line, untreated flies (-DOX) were sampled at their 50% survival and a sample was also taken for DOX treated (+DOX) flies at the same time point (same chronological age). An additional sample was taken for the treated flies (+DOX) at their 50% survival (same 'physiological age'). Venn diagram depicting gene expression changes due to MnSOD over-expression and the overlap with those that occur during normal aging [10]. Yellow highlighting indicates genes whose expression levels are altered at both time points. Green shading indicates genes identified as potential biomarkers of aging. Orange or blue text denotes genes up- or down-regulated, respectively, in a given condition or in the same direction in multiple conditions. Green or purple text denotes genes up- or down-regulated, respectively, in MnSOD over-expressing flies when the direction of change is opposite in old flies. Several representative functional categorizations are noted for the various gene sets. GPCR, GTP-binding protein-coupled receptor; Hsp, heat shock protein; TCA, tricarboxylic acid cycle

    MnSOD over-expression during adulthood is necessary and sufficient for lifespan extension and does not result in increased oxidative stress

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    <p><b>Copyright information:</b></p><p>Taken from "Transcriptional profiling of MnSOD-mediated lifespan extension in reveals a species-general network of aging and metabolic genes"</p><p>http://genomebiology.com/2007/8/12/R262</p><p>Genome Biology 2007;8(12):R262-R262.</p><p>Published online 9 Dec 2007</p><p>PMCID:PMC2246264.</p><p></p> Aconitase enzyme activity measured in mU/mg plotted against age for the following lines: control (a), (b), and (c). The effect of timing of MnSOD induction on lifespan for control (d), (e) and (f)

    Proposed mechanism for MnSOD-mediated mitochondria to nucleus signaling and crosstalk with the IIS pathway

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    <p><b>Copyright information:</b></p><p>Taken from "Transcriptional profiling of MnSOD-mediated lifespan extension in reveals a species-general network of aging and metabolic genes"</p><p>http://genomebiology.com/2007/8/12/R262</p><p>Genome Biology 2007;8(12):R262-R262.</p><p>Published online 9 Dec 2007</p><p>PMCID:PMC2246264.</p><p></p> The data suggest a model in which MnSOD catalyzed detoxification of superoxide results in increased intracellular hydrogen peroxide levels that mediate various signaling events. Such events include the activation of the JNK and NF-κB pathways. Pathway components that demonstrate increased expression due to MnSOD over-expression are highlighted in yellow. Note that genes up-regulated at both time points are indicated by black text, those up-regulated only at the first time point assayed are indicated by grey text, whereas those up-regulated only at the later time point are denoted by blue text. Solid lines indicate direct interactions, dashed lines indicate indirect interactions, dotted lines indicate translocation events, and '?' indicates hypothetical or speculative elements. The proposed retrograde signal from the mitochondria to the nucleus mediated by hydrogen peroxide is shown in red. Numerous genes are up-regulated as a result of these signaling events and some were also identified as being similarly altered in long-lived IIS mutants, suggesting their role as species-general lifespan effectors. These genes are indicated as are the biological processes that they contribute to. Hydrogen peroxide reversibly inhibits PTEN [105], an upstream inhibitor of IIS, resulting in activation of phosphoinositide 3-kinase (PI3K) signaling. In accordance with this, gene expression levels are up-regulated as are some downstream components of the IIS pathway in response to MnSOD over-expression. Increased IIS activity results in dFOXO inactivation and since MnSOD may be a direct transcriptional target, this suggests that feedback regulation may occur. The proposed feedback loop between MnSOD and the IIS pathway is also shown in red. Crosstalk between TOR and its binding partner, raptor, with the mitochondrion has been suggested [111], although the molecular basis has not been elucidated. As shown here, hydrogen peroxide may participate in this mechanism. ET, electron transport; ILP, insulin-like peptide

    Longevity promoting genes conserved between mutants and MnSOD over-expressing

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    <p><b>Copyright information:</b></p><p>Taken from "Transcriptional profiling of MnSOD-mediated lifespan extension in reveals a species-general network of aging and metabolic genes"</p><p>http://genomebiology.com/2007/8/12/R262</p><p>Genome Biology 2007;8(12):R262-R262.</p><p>Published online 9 Dec 2007</p><p>PMCID:PMC2246264.</p><p></p> and ortholog matches that are differentially expressed in response to MnSOD over-expression (both time points) and in mutants in a dependent manner. Expected values from BLASTP are indicated as follows: *5 × 1

    Candidate biomarkers of 'physiological age' include a highly regulated set of energy metabolism genes

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    <p><b>Copyright information:</b></p><p>Taken from "Transcriptional profiling of MnSOD-mediated lifespan extension in reveals a species-general network of aging and metabolic genes"</p><p>http://genomebiology.com/2007/8/12/R262</p><p>Genome Biology 2007;8(12):R262-R262.</p><p>Published online 9 Dec 2007</p><p>PMCID:PMC2246264.</p><p></p> GO classifications and functional overrepresentation of aging biomarkers. Orange or blue text denotes up- or down-regulated genes, respectively. 'Count' refers to the number of genes in the gene set belonging to a particular GO category. 'Ref' refers to the number of genes belonging to a particular GO category represented in the reference list (DrosGenome1 array)
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