New primary non-breast malignancies after breast cancer: ten years single institution follow-up

Background and Purpose: Breast cancer is the most common cancer in Croatian women. Due to improved diagnostic and treatment options women with breast cancer now live longer, which increases their risk of developing new primary malignancies. The aim of this study was to establish incidence of new primary non-breast malignancies after breast cancer diagnosis. Material and Methods: In the study cohort that included 215 consecutive patients treated for early breast cancer at University Hospital Center Zagreb, Croatia, 12 patients (5.58%) have developed new primary non-breast malignancy within nearly ten year follow-up. Results: Although the majority of studies found gynecological cancers to be the most common cancer site of new primary non-breast malignancies after breast cancer diagnosis, in our study most patients developed colorectal cancer. Conclusion: This is particularly interesting if you take into account that after breast cancer colorectal cancer is the second most common cancer in Croatian women. In order to stratify the risk for the development of new primary tumors it is necessary to further investigate the interaction of various factors that are thought to influence the evolvement of tumors

Experimental and Calculation Procedures for Molecular Lipophilicity: A Comparative Study for 3,3\u27-(2-Methoxybenzylidene)bis(4-hydroxycoumarin)

In our work, the log P value for 3,3\u27-(2-methoxybenzylidene)bis(4-hydroxycoumarin) (MBbisHC) was experimentally determined (»shake-flask« method) and calculated using six different computer programs: HyperChem 7.0 (based on atom contributions), XLOGP (based on atom contributions), KowWin (based on atom/fragment contributions), CLOGP (based on fragmental contributions), ALOGPS 2.1 (based on atom-type electrotopological-state indices and neural network modeling), and IA logP (based on atom-type electrotopological-state indices and neural network modeling). The experimental and calculated log P values were correlated. The best result was achieved using the HyperChem 7.0 program, which resulted in a nearly perfect agreement between the experimentally observed and calculated log P values

ULTRASOUND GUIDED BREAST BIOPSY – A RETROSPECTIVE STUDY AND LITERATURE REVIEW

Cilj. Svrha je istraživanja retrospektivno analizirati točnost i kliničku vrijednost biopsije širokom iglom pod kontrolom ultrazvuka (UZ) sumnjivih lezija u dojci. Materijali i metode. Retrospektivno su analizirani nalazi patohistološke analize 229 sumnjivih lezija u dojci koji su dobiveni biopsijom širokom iglom pod UZ kontrolom u razdoblju od 14 mjeseci. Sumnjive su lezije otkrivene mamografijom i/ili ultrazvukom (BI-RADS 4 ili 5). Ako je nakon biopsije postojala nepodudarnost radiološkog i patohistološkog nalaza, ponovila se biopsija širokom iglom ili se učinila kirurška biopsija lezije. U slučaju benignih lezija bolesnice su bile naručene na UZ kontrolu za šest mjeseci, a za granično maligne lezije i maligne lezije preporučena je kirurška ekscizija. Uspoređeni su nalazi biopsije širokom iglom i kirurške ekscizije. Rezultati. Patohistološkom analizom bioptiranih lezija otkrivene su 143 (62,4%) benigne lezije, 21 (9,2%) granično maligna lezija i 65 (28,4%) malignih lezija. Praćenjem, ponovljenom biopsijom ili kirurškom ekscizijom dokazana su četiri lažno negativna nalaza. Točnost biopsije širokom iglom pod UZ kontrolom iznosila je 98,3%. Zaključak. Biopsija širokom iglom pouzdana je metoda za postavljanje dijagnoze sumnjivih lezija u dojci bez značajnijih komplikacija.Aim. The purpose of this study is to determine the accuracy and clinical usefulness of ultrasound guided core biopsy for diagnosing suspicious radiologically detected breast lesions. Patients and methods. We retrospectively evaluated the results of percutaneous core biopsy with 14-gauge needles performed over a period of 14 months on 229 suspicious lesions detectable on mammography and/or ultrasound exam (BI-RADS 4 or 5). The imaging-histological concordance was ascertained for each lesion. In cases of discordance, repeat biopsy or surgical excision was performed. Six-month ultrasound control was recommended in cases of benign lesions. For borderline and malignant lesions a surgical excision was done. Concordance between biopsy results and subsequent examinations (surgical excision or follow-up) was also evaluated. Results. Histological analysis of core biopsy samples showed 143 (62.4%) benign lesions, 21 (9.2%) borderline lesions and 65 (28.4%) malignant lesions. Follow-up, repeated biopsy, or surgical excision showed four false negative cases. Accuracy of ultrasound guided core biopsy was 98.3%. Conclusion. Ultrasound guided core biopsy is a safe and reliable method for diagnosing suspicious breast lesions without any significant complications as was reported in previous studies

MULTIPLE PRIMARY MALIGNANCIES

Multipli primarni tumori koji se javljaju kod istog bolesnika, metakrono ili sinkrono, relativno su rijedak događaj s porastom učestalosti posljednjih desetljeća. Cilj je ovog istraživanja utvrditi njihovu učestalost kod bolesnika liječenih hospitalno u Zavodu za radioterapijsku onkologiju Klinike za onkologiju Medicinskog fakulteta Sveučilišta u Zagrebu, KBC Zagreb u periodu od 2003. do 2009. godine. Učestalost je multiplih malignih tumora u navedenom periodu bila 2,4%. Od ukupno 103 bolesnika 97 je imalo dva, a 6 tri primarna tumora. Metakronih je tumora bilo 88, a sinkronih 20. Učestalost im je bila veća kod žena nego kod muškaraca, a i pojavljivali su se ranije kod žena nego kod muškaraca. Najčešće su kombinacije prvog i drugoga metakronog tumora kod muškaraca bile: rak prostate-maligni tumor probavnog sustava (osobito rak rektuma i debelog crijeva) i obrnutim redoslijedom te hematološke zloćudne bolesti-maligni tumor probavnog sustava; a kod žena: rak dojke-rak kontralateralne dojke i hematološke zloćudne bolesti (osobito ne-Hodgkinov limfom)-rak dojke. Valja očekivati da će učestalost bolesnika s višestrukim primarnim tumorima rasti, i zbog programa ranog otkrivanja tumora i zbog uspješnijeg liječenja i dužeg očekivanog trajanja života.Multiple primary malignancies, metachronous or synchronous, in a single patient are relatively rare event with the increase of incidence in recent decades. The aim of this research is to study their incidence in patients hospitalized at the Division of Radiotherapy, Department of Oncology, University of Zagreb, School of medicine, University Hospital Centre Zagreb from 2003 to 2009. The incidence of multiple primary malignancies was 2.4%. Among 103 patients, 97 had two, and 6 three primary tumors. Eighty-three cases were metachronous, while 20 cases were synchronous malignancies. The frequency was higher in females than males and their age at diagnosis of tumors was younger than in males. The most common tumor combinations in males were: prostate cancer-digestive system malignancy (especially colorectal cancer) and viceversa, and hematological malignant tumors-digestive system malignancy; while in women there were: breast cancer-cancer of contralateral breast and hematological malignant tumors (especially lymphoma non Hodgkin)-breast cancer. The incidence of multiple primary malignancies is expected to increase due to the better screening programs for early detection of malignancies as well as considerable improvement in their treatment and longer life expectancy

High expression of MAGE-A10 cancer-testis antigen in triple-negative breast cancer

Recent studies indicate that ER/PR/HER-2-negative (triple-negative, TN) breast cancers may be "CTA-rich" tumors, suggesting the possibility of CTA-based cancer vaccines as a treatment option for patients bearing these tumors. MAGE-A10 together with NY-ESO-1 is probably the most immunogenic CTA, representing a potentially highly attractive target of active specific immunotherapies. Paraffin-embedded tumor sections were collected retrospectively from 165 breast cancer patients diagnosed between 2002 and 2003. Immunohistochemical staining for MAGE-A10 and NY-ESO-1 was performed. The expression of MAGE-A10 and NY-ESO-1 was correlated with other clinicopathological variables. MAGE-A10 expression (score </= 2+) was detected in 105/164 (64%), and NY-ESO-1 expression (score </= 2+) was observed in 14/164 (8.5%) patients. No correlation between MAGE-A10 and NY-ESO-1 expression and tumor size, tumor grade, Ki-67 and lymph nodes status was detectable. MAGE-A10 expression was significantly associated with ER-negative (P = 0.002), PR-negative (P = 0.002) and HER-2-negative (P = 0.044) tumors. We clearly showed that MAGE-A10 is frequently expressed in the group of TN patients, where the majority (85.7%) of tumors express this CTA. Because of limited therapeutic options for the triple-negative breast cancer, the frequent expression of MAGE-A10 CTA in these cancers may offer the opportunity for a much needed additional treatment for this group of patients

Biopsija dojke pod kontrolom ultrazvuka - retrospektivna studija i pregled literature [Ultrasound guided breast biopsy - a retrospective study and literature review]

AIM: The purpose of this study is to determine the accuracy and clinical usefulness of ultrasound guided core biopsy for diagnosing suspicious radiologically detected breast lesions. ----- PATIENTS AND METHODS: We retrospectively evaluated the results of percutaneous core biopsy with 14-gauge needles performed over a period of 14 months on 229 suspicious lesions detectable on mammography and/or ultrasound exam (BI-RADS 4 or 5). The imaging-histological concordance was ascertained for each lesion. In cases of discordance, repeat biopsy or surgical excision was performed. Six-month ultrasound control was recommended in cases of benign lesions. For borderline and malignant lesions a surgical excision was done. Concordance between biopsy results and subsequent examinations (surgical excision or follow-up) was also evaluated. ----- RESULTS: Histological analysis of core biopsy samples showed 143 (62.4%) benign lesions, 21 (9.2%) borderline lesions and 65 (28.4%) malignant lesions. Follow-up, repeated biopsy, or surgical excision showed four false negative cases. Accuracy of ultrasound guided core biopsy was 98.3%. ----- CONCLUSION: Ultrasound guided core biopsy is a safe and reliable method for diagnosing suspicious breast lesions without any significant complications as was reported in previous studies