Secretome Data from Trichoderma Reesei and Aspergillus Niger Cultivated in Submerged and Sequential Fermentation Methods

The cultivation procedure and the fungal strain applied for enzyme production may influence levels and profile of the proteins produced. The proteomic analysis data presented here provide critical informa- tion to compare proteins secreted by Trichoderma reesei and Aspergillus niger when cultivated through submerged and sequential fermenta- tion processes, using steam-explosion sugarcane bagasse as inducer for enzyme production. The proteins were organized according to the families described in CAZy database as cellulases, hemicellulases, proteases/peptidases, cell-wall-protein, lipases, others (catalase, ester- ase, etc.), glycoside hydrolases families, predicted and hypothetical proteins. Further detailed analysis of this data is provided in “Secre- tome analysis of Trichoderma reesei and Aspergillus niger cultivated by submerged and sequential fermentation process: enzyme production for sugarcane bagasse hydrolysis” C. Florencio, F.M. Cunha, A.C Badino, C.S. Farinas, E. Ximenes, M.R. Ladisch (2016) [1]

Optimisation of the glycerol-to-ornithine molar ratio in the feed medium for the continuous production of clavulanic acid by Streptomyces clavuligerus

Amino acids are well metabolized by Streptomyces clavuligerus during the production of clavulanic acid using glycerol as main carbon and energy source. However, only a few amino acids such as arginine and ornithine are favorable for CA biosynthesis. The aim of this work was to optimize the glycerol:ornithine molar ratio in the feed medium containing only these compounds to maximize CA production in continuous cultivation. A minimum number of experiments were performed by means of a simple two-level full-factorial central composite design to investigate the combined effect of glycerol and ornithine feeding on the CA concentration during the intermittent and continuous process in shake-flasks. Statistical analysis of the experimental data using the response surface methodology showed that a glycerol-to-ornithine molar ratio of approximately 40:1 in the feed medium resulted in the highest CA concentration when fermentation was stopped. Under these optimized conditions, in bench-scale fermentor runs, the CA concentration reached more than double the concentration obtained in shake-flasks runs. (C) 2009 Elsevier B.V. All rights reserved

High Lipase Production from Geotrichum candidum in Reduced Time using Cottonseed Oil: Optimization, Easy Purification and Specificity Characterization

Despite the wide variety of potential applications of lipases within industrial processes, the high cost of production and purification is still their main limiting factor. The aim of this work is to optimize the production of Geotrichum candidum lipase (GCL) using submerged fermentation with a combination of statistical experimental design and surface methodology analysis, in order to give a higher production within a shorter time at the lowest possible cost and easy purification. Cottonseed oil, a low-cost by-product of cotton processing, was used as both an inducer and a carbon source. A maximum lipase activity of 27.17 IUmL-1 was achieved after 30h fermentation in a 5L stirred tank bioreactor under optimal conditions: 2.3% (m/v) of casein peptone, 0.8% (v/v) of cottonseed oil and 0.05% (m/v) of MgSO4 and NaNO3. The lipase purification in a single step by immobilization on PHB particles was verified. The combination of these two steps allowed a significant decrease in this lipase cost of production. Moreover, the produced lipase showed high specificity to hydrolyze long-chain fatty acids with cis-9 double bonds, such as oleic and linoleic acids, having an excellent potential for modifying oils in order to produce different bio-products in industrial applications