70 years later – New research at Holley Shelter, a Middle and Later Stone Age site in KwaZulu-Natal, South Africa

In 1952, Gordon Cramb published the first report on his excavations at Holley Shelter, in KwaZulu-Natal, South Africa. Although Cramb’s work implied organic preservation associated with a unique stone tool assemblage from a Middle Stone Age (MSA) context, Holley Shelter disappeared from the archaeological landscape until 2015, when we provided a reinvestigation of the lithic assemblages from the 1950s. Our study indicated a tentative chronological framework around Marine Isotope Stage 3 (MIS3) for the human occupation of the site. However, Cramb’s excavations did not produce reliable chronometric ages and the botanical and zoological finds from Holley Shelter remain for the most part unstudied. This is problematic as the site constitutes one of the few inland MSA rock shelters of KwaZulu-Natal featuring organic preservation. In 2022, 70 years after Cramb’s first report, we started a new research project focusing on renewed excavations to obtain archaeological remains from a controlled stratigraphy, absolute chronometric ages and reliable data on the palaeoenvironment at the border between the coastal belt and the midlands of KwaZulu-Natal. Here, we provide initial results from the first field campaign in 2022 on the stratigraphic sequence and archaeological finds and discuss their implications for future multidisciplinary research. Significance: Environmental change can have a strong impact on hunter-gatherer behaviour, migration and technological choices. It is thus crucial to contextualise archaeological material with a strong palaeoenvironmental record. The inland of KwaZulu-Natal, South Africa, represents an understudied region in terms of Stone Age archaeological sites and palaeoenvironmental record. Holley Shelter is one of the few sites in South Africa with excellent organic preservation and a deep stratigraphic record, which provide a great opportunity to investigate human-landscape interaction and technological change throughout the Middle and Later Stone Age

The Forgotten Kingdom.: New investigations in the prehistory of Eswatini

The kingdom of Eswatini provides a rich archaeological sequence covering all time periods from the Early Stone Age to the Iron Age. For over 27 years though, no or very little archaeological research was conducted in the country. In the scope of a new project funded by the German Research Foundation (DFG) we aim to re-excavate and re-date Lion Cavern, the potentially oldest ochre mine in the world. In addition, we conduct a largescale geological survey for outcrops of ochre and test their geochemical signatures for comparative studies with archaeological ochre pieces from MSA and LSA assemblages in Eswatini. Here we present a review of the research history of the kingdom and some preliminary results from our ongoing project

Fast network centrality analysis using GPUs

<p>Abstract</p> <p>Background</p> <p>With the exploding volume of data generated by continuously evolving high-throughput technologies, biological network analysis problems are growing larger in scale and craving for more computational power. General Purpose computation on Graphics Processing Units (GPGPU) provides a cost-effective technology for the study of large-scale biological networks. Designing algorithms that maximize data parallelism is the key in leveraging the power of GPUs.</p> <p>Results</p> <p>We proposed an efficient data parallel formulation of the All-Pairs Shortest Path problem, which is the key component for shortest path-based centrality computation. A betweenness centrality algorithm built upon this formulation was developed and benchmarked against the most recent GPU-based algorithm. Speedup between 11 to 19% was observed in various simulated scale-free networks. We further designed three algorithms based on this core component to compute closeness centrality, eccentricity centrality and stress centrality. To make all these algorithms available to the research community, we developed a software package <it>gpu</it>-<it>fan </it>(GPU-based Fast Analysis of Networks) for CUDA enabled GPUs. Speedup of 10-50× compared with CPU implementations was observed for simulated scale-free networks and real world biological networks.</p> <p>Conclusions</p> <p><it>gpu</it>-<it>fan </it>provides a significant performance improvement for centrality computation in large-scale networks. Source code is available under the GNU Public License (GPL) at <url>http://bioinfo.vanderbilt.edu/gpu-fan/</url>.</p

Flexible Usage and Interconnectivity of Diverse Cell Death Pathways Protect against Intracellular Infection.

Programmed cell death contributes to host defense against pathogens. To investigate the relative importance of pyroptosis, necroptosis, and apoptosis during Salmonella infection, we infected mice and macrophages deficient for diverse combinations of caspases-1, -11, -12, and -8 and receptor interacting serine/threonine kinase 3 (RIPK3). Loss of pyroptosis, caspase-8-driven apoptosis, or necroptosis had minor impact on Salmonella control. However, combined deficiency of these cell death pathways caused loss of bacterial control in mice and their macrophages, demonstrating that host defense can employ varying components of several cell death pathways to limit intracellular infections. This flexible use of distinct cell death pathways involved extensive cross-talk between initiators and effectors of pyroptosis and apoptosis, where initiator caspases-1 and -8 also functioned as executioners when all known effectors of cell death were absent. These findings uncover a highly coordinated and flexible cell death system with in-built fail-safe processes that protect the host from intracellular infections.Wellcome Trus

70 years later – New research at Holley Shelter, a Middle and Later Stone Age site in KwaZulu-Natal, South Africa

In 1952, Gordon Cramb published the first report on his excavations at Holley Shelter, in KwaZulu-Natal, South Africa. Although Cramb’s work implied organic preservation associated with a unique stone tool assemblage from a Middle Stone Age (MSA) context, Holley Shelter disappeared from the archaeological landscape until 2015, when we provided a reinvestigation of the lithic assemblages from the 1950s. Our study indicated a tentative chronological framework around Marine Isotope Stage 3 (MIS3) for the human occupation of the site. However, Cramb’s excavations did not produce reliable chronometric ages and the botanical and zoological finds from Holley Shelter remain for the most part unstudied. This is problematic as the site constitutes one of the few inland MSA rock shelters of KwaZulu-Natal featuring organic preservation. In 2022, 70 years after Cramb’s first report, we started a new research project focusing on renewed excavations to obtain archaeological remains from a controlled stratigraphy, absolute chronometric ages and reliable data on the palaeoenvironment at the border between the coastal belt and the midlands of KwaZulu-Natal. Here, we provide initial results from the first field campaign in 2022 on the stratigraphic sequence and archaeological finds and discuss their implications for future multidisciplinary research. Significance: Environmental change can have a strong impact on hunter-gatherer behaviour, migration and technological choices. It is thus crucial to contextualise archaeological material with a strong palaeoenvironmental record. The inland of KwaZulu-Natal, South Africa, represents an understudied region in terms of Stone Age archaeological sites and palaeoenvironmental record. Holley Shelter is one of the few sites in South Africa with excellent organic preservation and a deep stratigraphic record, which provide a great opportunity to investigate human-landscape interaction and technological change throughout the Middle and Later Stone Age

Distribution of techno-functional tool classes.

<p>Rounded percentages are given in brackets.</p

Cortex cover on artifacts for each assemblage and for the total sample of dolerite and hornfels.

<p>Rounded percentages are given in brackets.</p

Distribution of single finds (>25 mm) and small debitage (<25 mm).

<p>Distribution of single finds (>25 mm) and small debitage (<25 mm).</p

Excavation area and stratigraphic sections of the “post-HP” sequence from Sibudu.

<p>Upper left: Excavation grid. The lithic assemblages from the Sibudan come from the “Eastern Excavation”. Right: Sketch of the stratigraphic section of the eastern profile (C4, after Wadley). The complete “post-HP” sequence is highlighted in orange (layers BSP-BR Under YA2). Bottom left: Photograph depicting the stratigraphic section of the northern profile (C3) during excavations in 2013. The white lines mark the seven uppermost layers of the “post-HP”, or Sibudan, sequence from the top of BSP until the bottom of BM. Note the very fine lamination of archaeological layers in different colors caused by frequent combustion features (photograph by M. Will).</p