Strategies for Turning Ideas into Business at Universities

Universities are well known for undertaking research projects in various sectors and making technological breakthroughs but few universities have focused on actually turning ideas into businesses. More and more universities are beginning to realize that it is no longer adequate to simply produce graduates and doctoral theses. They must play a greater role in generating wealth for their country and contributing to society by ensuring that promising inventions and other creative ideas do not get lost but be developed into marketable products and services. As a result, there is currently a growing trend whereby universities are taking steps to become more entrepreneurial by encouraging students and staff to generate creative ideas and commercialize their ideas. This paper examines the programmes of Universities at the International Level that have been promoting entrepreneurship development and recommends intervention areas to increase the capacity of a University to derive the benefits from an active business commercialization process. The key recommendations include the development of an entrepreneurial vision for the University, cultivating partnerships with private and public sector organizations and   creating linkages that will promote mentorship and funding opportunities.  Keywords: Entrepreneurial University, Business Commercialization, Innovation, Triple Helix Mode

Immune response to BK virus reactivation in renal transplant recipients

BK virus, a virus of the polyomaviridae family, is a latent infection in up to 70% of the general population. However, it has been an emerging cause of renal allograft dysfunction in kidney transplant recipients. Existing research has elucidated the risk factors that may precipitate BK viremia and BK Virus Associated Nephropathy. Research into the humoral and adaptive immune response to BK reactivation is still ongoing. This thesis is part of a larger study that aims to further elucidate the various aspects of the cellular response to BK reactivation. It focuses on the methods of quantitation of BK virus pre-transplant, 1 month, 3 months, 6 months and 12 months post-transplant. Laboratory qPCR and clinical PCR assays were conducted and analyzed. Various aspects of the cellular and humoral response were also examined and analyzed. As there exists no standard protocol for BK surveillance in kidney transplant recipients, analysis of these data will undoubtedly pave the way for future research in this field. It was concluded that BKV levels in plasma and urine should be more stringently monitored and the required assays perfected for a higher degree of sensitivity

The Role of the Caribbean Entrepreneur in Economic Development - Strategy and Process

The Caribbean is a diverse group of countries, both culturally and economically. Most of them, however, face daunting development challenges, including pervasive crime, unemployment and an underdeveloped private sector. As Caribbean intellectuals and policy makers confront the question of how to create employment so that people can meet their basic needs they have increasingly endorsed the development of self-reliance through the creation of small business operations as the panacea for improving the economic marginalisation of the masses. The current academic debate focuses on the factors that are impeding the growth and development of the Caribbean region and what frameworks can be designed to augment an entrepreneurial culture whilst simultaneously engaging natural and other resources. These are the fundamental issues that this research paper will share perspectives and research, with the aim of identifying components that would leverage the resources of the region in a positive manner. Keywords: Entrepreneurship Policy, Caribbean Development, Economic Strategy, Entrepreneu

Implementing Public Policy for Business Development: The National Entrepreneurship Development Company

Entrepreneurship creation has been supported by many entrepreneurship development policies which have been implemented with various cross-cutting mechanisms. Such policies have been designed and executed within the international arena with mixed results in shaping the entrepreneurial growth rate. There has been limited research in the operational process of implementing public policy for stimulating entrepreneurship within developing countries and this research paper addresses this gap with an examination of the experiences of a selected public sector organization called the National Entrepreneurship Development Company Limited (NEDCO) that has the mandate for implementing the entrepreneurship policy of Trinidad and Tobago. The research has revealed that central pillars for success in public policy implementation include public sector alignment, customer responsiveness, the adoption of market orientation in operations, establishing strategic alliances within the business enterprise eco-systems, and creating monitoring and evaluation frameworks that engage a wide stake-holder net with a benchmarking and 360 degree approach. Research paper Reference to this paper should be made as follows: Ramkissoon-Babwah, N. Mc David, J. (2017). Implementing Public Policy for Business Development: The National Entrepreneurship Develop-ment Company, Journal of Entrepreneurship, Business and Economics, Vol. 5, No. 1, pp. 140–156

Towards a hydrodynamic operational model of the Algarve coast and St. Vincent Cape

This study attempts to implement a hydrodynamic operational model which can ultimately be used for projecting oil spill dispersal patterns and also sewage, pollution and can also be used in wave forecasting. A two layer nested model was created using MOHID Water, which is powerful ocean modelling software. The first layer (father) is used to impose the boundary conditions for the second layer (son). This was repeated for two different wind dominant regimes, Easterly and Westerly winds respectively. A qualitative comparison was done between measured tidal data and the tidal output. Sea surface temperature was also qualitatively compared with the model’s results. The results from both simulations were analysed and compared to historical literature. The comparison was done at the surface layer, 100 metre depth and at 800m depth. In the surface layer the first simulation generated an upwelling event near Cape St. Vincent and within the Algarve. The second simulation generated a non-upwelling event within which the surface was flow reversed and the warm water mass was along the Algarve coastline and evening turning clockwise around Cape St. Vincent. At the 100 metre depth for both simulations, velocity vortexes were observed near Cape St. Vincent travelling northerly and southerly at various instances. At 800metre depth a strong oceanic flow was observed moving north westerly along the continental shelf

KISS1R : hallmarks of an effective regulator of the neuroendocrine axis

Kisspeptin (KP) is now well recognized as a potent stimulator of gonadotropin-releasing hormone (GnRH) secretion and thereby a major regulator of the neuroendocrine-reproductive axis. KP signals via KISS1R, a G protein-coupled receptor (GPCR) that activates the G proteins Gαq/11. Modulation of the interaction of KP with KISS1R is therefore a potential new therapeutic target for stimulating (in infertility) or inhibiting (in hormone-dependent diseases) the reproductive hormone cascade. Major efforts are underway to target KISS1R in the treatment of sex steroid hormone-dependent disorders and to stimulate endogenous hormonal responses along the neuroendocrine axis as part of in vitro fertilization protocols. The development of analogs modulating KISS1R signaling will be aided by an understanding of the intracellular pathways and dynamics of KISS1R signaling under normal and pathological conditions. This review focuses on KISS1R recruitment of intracellular signaling (Gαq/11- and β-arrestin-dependent) pathways that mediate GnRH secretion and the respective roles of rapid desensitization, internalization, and recycling of resensitized receptors in maintaining an active population of KISS1R at the cell surface to facilitate prolonged KP signaling. Additionally, this review summarizes and discusses the major findings of an array of studies examining the desensitization of KP signaling in man, domestic and laboratory animals. This discussion highlights the major effects of ligand efficacy and concentration and the physiological, developmental, and metabolic status of the organism on KP signaling. Finally, the potential for the utilization of KP and analogs in stimulating and inhibiting the reproductive hormone cascade as an alternative to targeting the downstream GnRH receptor is discussed.Natural Sciences and Engineering Research Council of Canada (RGPIN/327334-2011), National Research Foundation and Medical Research Council of South Africa, and the Universities of Pretoria and Cape Town.http://www.karger.com/Journal/Home/223855hb201

Uterine kisspeptin receptor critically regulates epithelial estrogen receptor α transcriptional activity at the time of embryo implantation in a mouse model

Embryo implantation failure is a major cause of infertility in women of reproductive age and a better understanding of uterine factors that regulate implantation is required for developing effective treatments for female infertility. This study investigated the role of the uterine kisspeptin receptor (KISS1R) in the molecular regulation of implantation in a mouse model. To conduct this study, a conditional uterine knockout (KO) of Kiss1r was created using the Pgr-Cre (progesterone receptor-CRE recombinase) driver. Reproductive profiling revealed that while KO females exhibited normal ovarian function and mated successfully to stud males, they exhibited significantly fewer implantation sites, reduced litter size and increased neonatal mortality demonstrating that uterine KISS1R is required for embryo implantation and a healthy pregnancy. Strikingly, in the uterus of Kiss1r KO mice on day 4 (D4) of pregnancy, the day of embryo implantation, KO females exhibited aberrantly elevated epithelial ERα (estrogen receptor α) transcriptional activity. This led to the temporal misexpression of several epithelial genes [Cftr (Cystic fibrosis transmembrane conductance regulator), Aqp5 (aquaporin 5), Aqp8 (aquaporin 8) and Cldn7 (claudin 7)] that mediate luminal fluid secretion and luminal opening. As a result, on D4 of pregnancy, the lumen remained open disrupting the final acquisition of endometrial receptivity and likely accounting for the reduction in implantation events. Our data clearly show that uterine KISS1R negatively regulates ERα signaling at the time of implantation, in part by inhibiting ERα overexpression and preventing detrimentally high ERα activity. To date, there are no reports on the regulation of ERα by KISS1R; therefore, this study has uncovered an important and powerful regulator of uterine ERα during early pregnancy

The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation

Background: Expression of kisspeptin (protein) and Kiss1r (mRNA) was recently documented in the mouse uterus on D4 of pregnancy (the day of embryo implantation) suggesting that the uterine-based kisspeptin (KP)/kisspeptin receptor (KISS1R) signaling system regulates embryo implantation. Despite this important suggestion, it was never demonstrated that the uterus actually exhibits a functional KP/KISS1R signaling system on D4 of pregnancy. Thus, the goal of this study was to determine whether a functional KP/KISS1R signaling system exists in the mouse uterus on D4 of pregnancy. Findings: Since kisspeptin/KISS1R signaling triggers the phosphorylation of the mitogen-activated protein kinases p38 and ERK1/2, through immunohistochemical analyses, we determined whether exogenously administered kisspeptin could trigger p38 and ERK1/2 phosphorylation in the uterus on D4 of pregnancy. The results clearly demonstrated that kisspeptin could and that its effects were mediated via KISS1R. Additionally, the robust kisspeptin-triggered response was observed in the pregnant uterus only. Finally, it was demonstrated that on D4 of pregnancy the Kiss1 null uterus expresses functional KISS1R molecules capable of mediating the effects of kisspeptin. Conclusions: These results lead us to conclude that on D4 of pregnancy, the mouse uterus expresses a functional KP/KISS1R signaling system strengthening the possibility that this signaling system regulates embryo implantation. These findings strengthen the rationale for determining whether such a functional system exists in the uterus of the human female and if so, what role it might play in human pregnancy

GPR54 Regulates ERK1/2 Activity and Hypothalamic Gene Expression in a Gαq/11 and β-Arrestin-Dependent Manner

G protein-coupled receptor 54 (GPR54) is a Gq/11-coupled 7 transmembrane-spanning receptor (7TMR). Activation of GPR54 by kisspeptin (Kp) stimulates PIP2 hydrolysis, Ca2+ mobilization and ERK1/2 MAPK phosphorylation. Kp and GPR54 are established regulators of the hypothalamic-pituitary-gonadal (HPG) axis and loss-of-function mutations in GPR54 are associated with an absence of puberty and hypogonadotropic hypogonadism, thus defining an important role of the Kp/GPR54 signaling system in reproductive function. Given the tremendous physiological and clinical importance of the Kp/GPR54 signaling system, we explored the contributions of the GPR54-coupled Gq/11 and β-arrestin pathways on the activation of a major downstream signaling molecule, ERK, using Gq/11 and β-arrestin knockout mouse embryonic fibroblasts. Our study revealed that GPR54 employs the Gq/11 and β-arrestin-2 pathways in a co-dependent and temporally overlapping manner to positively regulate ERK activity and pERK nuclear localization. We also show that while β-arrestin-2 potentiates GPR54 signaling to ERK, β-arrestin-1 inhibits it. Our data also revealed that diminished β-arrestin-1 and -2 expression in the GT1-7 GnRH hypothalamic neuronal cell line triggered distinct patterns of gene expression following Kp-10 treatment. Thus, β-arrestin-1 and -2 also regulate distinct downstream responses in gene expression. Finally, we showed that GPR54, when uncoupled from the Gq/11 pathway, as is the case for several naturally occurring GPR54 mutants associated with hypogonadotropic hypogonadism, continues to regulate gene expression in a G protein-independent manner. These new and exciting findings add significantly to our mechanistic understanding of how this important receptor signals intracellularly in response to kisspeptin stimulation

Uterine aquaporin expression is dynamically regulated by estradiol and progesterone and ovarian stimulation disrupts embryo implantation without affecting luminal closure

The study investigated the effect of normal and supraphysiological (resulting from gonadotropin-dependent ovarian stimulation) levels of estradiol (E2) and progesterone (P4) on mouse uterine aquaporin gene/protein (Aqp/AQP) expression on Day 1 (D1) and D4 of pregnancy. The study also examined the effect of ovarian stimulation on uterine luminal closure and uterine receptivity on D4 of pregnancy and embryo implantation on D5 and D7 of pregnancy. These analyses revealed that the expression of Aqp3, Aqp4, Aqp5 and Aqp8 is induced by E2 while the expression of Aqp1 and Aqp11 is induced by P4. Additionally, P4 inhibits E2 induction of Aqp3 and Aqp4 expression while E2 inhibits Aqp1 and Aqp11 expression. Aqp9, however, is constitutively expressed. Ovarian stimulation disrupts Aqp3, Aqp5 and Aqp8 expression on D4 and AQP1, AQP3 and AQP5 spatial expression on both D1 and D4, strikingly so in the myometrium. Interestingly, while ovarian stimulation has no overt effect on luminal closure and uterine receptivity, it reduces implantation events, likely through a disruption in myometrial activity and embryo development. The wider implication of this study is that ovarian stimulation, which results in supraphysiological levels of E2 and P4 and changes (depending on the degree of stimulation) in the E2:P4 ratio, triggers abnormal expression of uterine AQP during pregnancy, and this is associated with implantation failure. These findings lead us to recognize that abnormal expression would also occur under any pathological state (such as endometriosis) that is associated with changes in the normal E2:P4 ratio. Thus, infertility among these patients might in part be linked to abnormal uterine AQP expression