Studies on \u3cem\u3eIn Vitro\u3c/em\u3e Antimicrobial Activity of Ethanol Extract of \u3cem\u3eRauvolfia tetraphylla\u3c/em\u3e

The antimicrobial activity of ethanol extract obtained from Rauvolfia tetraphylla was tested against bacterial species of Escherichia coli, Streptococcus lactis, Enterobacter aerogenes, Alcaligenes faecalis, Pseudomonas aeruginosa, Proteus vulgaris, and fungal species of Fusarium oxysporum, Alternaria helianthii, Curvularia lunata, Aspergillus niger, and Penicillium spp. Better antimicrobial activity was observed when the extracts showed maximum activity against E. coli, Enterobacter aerogenes, and Alcaligenes faecalis. Among different fungi tested, A. niger and Penicillium spp were found to be more sensitive to crude extract when compared to others

Callus Induction and Plant Regeneration of \u3cem\u3eVigna mungo\u3c/em\u3e (L.) Hepper via Half Seed Explant

The present study optimized the regeneration protocol by using half seed explant in Vigna mungo (L.) Hepper organogenesis. Half seed explants were inoculated onto B5 medium supplemented with kinetin (4.7 μM to 23.5 μM), 6- benzyladenine (4.4 μM to 22.2 μM), naphthaleneacetic acid (5.4 μM to 27.0 μM), indolebutyric acid (4.9 μM to 24.5 μM) and 2,4- dichlorophenoxyacetic acid (4.5 μM to 22.5 μM). Callus initiation was observed in all media evaluated and the highest cell proliferation was obtained from explants cultivated in the presence of 13.3 μM BAP and 13.5 μM 2,4-D. Shoot induction was obtained from callus induced on 13.3 μM BAP and 13.5 μ 2,4-D at 6 weeks after transferring the callus to a B5 medium supplemented with 13.3 μM BAP. Roots were induced from shoots on B5 media with indolebutyric acid (IBA, 14.7 μM) and then regenerated plants were hardened and acclimated in greenhouse conditions

Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum basilicum

Ocimum basilicum leaf extracts of were investigated for phytochemical constituent and antioxidant activity. Tests for tannins, steroids, terpenoids, flavonoids and cardiac glycosides were positive in both ethanolic and aqueous extracts. The ethanolic extract of O. basilicum had a DPPH scavenging activity of 85.2% at 250μg/ml and a reductive potential of 0.79 at 100 μg/ml. These values were comparable with those of gallic acid, 91.1% at 250 μg/ml and ascorbic acid, 0.76 at 60 μg/ml as standards for DPPH scavenging activity and reductive potential, respectively. These findings suggest that the rich phytochemical content of O. basilicum and its good antioxidant activity may be responsible for its popular and wide traditional use

Evaluation of behavioural and antioxidant activity of Cytisus scoparius Link in rats exposed to chronic unpredictable mild stress

<p>Abstract</p> <p>Background</p> <p>Various human diseases have oxidative stress as one of their component. Many herbs have been reported to exhibit properties that combat oxidative stress through their active constituents such as flavonoids, tannins, phenolic compounds etc. <it>Cytisus scoparius </it>(CS) Link, (Family: Leguminosae), also called <it>Sarothamnus scoparius</it>, has been shown in <it>invitro </it>experiments to be endowed with anti-diabetic, hypnotic and sedative and antioxidant activity. Therefore this study was carried out to evaluate CS for its anxiolytic, antidepressant and anti-oxidant activity in stressed rats.</p> <p>Methods</p> <p>60% methanolic extract of CS was quantified for phenolic content by Folin-Ciocalteau's method. Chronic unpredictable mild stress (CMS) was employed to induce stress in rats. CS (125 and 250 mg/kg, p.o) and diazepam (DZM) (2 mg/kg, p.o) was administered during the 21 day stress exposure period. Anxiolytic and antidepressant activities of CS were assessed in open field exploratory and behavioural despair paradigms, respectively. Plasma glucose and total lipids; endogenous antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT); non-enzymic-ascorbic acid and thiobarbituric acid reactive substances (TBARS) levels were measured in brain, kidneys and adrenals using standard protocols to assess the effect of CS.</p> <p>Results</p> <p>Total phenolic content of CS was found to be 8.54 ± 0.16% w/w. CMS produced anxiogenic and depressive behaviour in experimental rats with metabolic disturbance. Significant decrease in SOD, CAT levels and increase in lipid peroxidation level was observed in stressed rats. CS administration for 21 days during stress exposure significantly increased the ambulatory behaviour and decreased the freezing time in open field behaviour. In behavioural despair test no significant alteration in the immobility period was observed. CS also improved SOD, CAT, and ascorbic acid level and controlled the lipid peroxidation in different tissues.</p> <p>Conclusion</p> <p>CS possesses anti-stress and moderate anxiolytic activity which may be due, in part, to its antioxidant effect that might warrant further studies.</p

Global, regional, and national burden of disorders affecting the nervous system, 1990–2021: a systematic analysis for the Global Burden of Disease Study 2021

BackgroundDisorders affecting the nervous system are diverse and include neurodevelopmental disorders, late-life neurodegeneration, and newly emergent conditions, such as cognitive impairment following COVID-19. Previous publications from the Global Burden of Disease, Injuries, and Risk Factor Study estimated the burden of 15 neurological conditions in 2015 and 2016, but these analyses did not include neurodevelopmental disorders, as defined by the International Classification of Diseases (ICD)-11, or a subset of cases of congenital, neonatal, and infectious conditions that cause neurological damage. Here, we estimate nervous system health loss caused by 37 unique conditions and their associated risk factors globally, regionally, and nationally from 1990 to 2021.MethodsWe estimated mortality, prevalence, years lived with disability (YLDs), years of life lost (YLLs), and disability-adjusted life-years (DALYs), with corresponding 95% uncertainty intervals (UIs), by age and sex in 204 countries and territories, from 1990 to 2021. We included morbidity and deaths due to neurological conditions, for which health loss is directly due to damage to the CNS or peripheral nervous system. We also isolated neurological health loss from conditions for which nervous system morbidity is a consequence, but not the primary feature, including a subset of congenital conditions (ie, chromosomal anomalies and congenital birth defects), neonatal conditions (ie, jaundice, preterm birth, and sepsis), infectious diseases (ie, COVID-19, cystic echinococcosis, malaria, syphilis, and Zika virus disease), and diabetic neuropathy. By conducting a sequela-level analysis of the health outcomes for these conditions, only cases where nervous system damage occurred were included, and YLDs were recalculated to isolate the non-fatal burden directly attributable to nervous system health loss. A comorbidity correction was used to calculate total prevalence of all conditions that affect the nervous system combined.FindingsGlobally, the 37 conditions affecting the nervous system were collectively ranked as the leading group cause of DALYs in 2021 (443 million, 95% UI 378–521), affecting 3·40 billion (3·20–3·62) individuals (43·1%, 40·5–45·9 of the global population); global DALY counts attributed to these conditions increased by 18·2% (8·7–26·7) between 1990 and 2021. Age-standardised rates of deaths per 100 000 people attributed to these conditions decreased from 1990 to 2021 by 33·6% (27·6–38·8), and age-standardised rates of DALYs attributed to these conditions decreased by 27·0% (21·5–32·4). Age-standardised prevalence was almost stable, with a change of 1·5% (0·7–2·4). The ten conditions with the highest age-standardised DALYs in 2021 were stroke, neonatal encephalopathy, migraine, Alzheimer's disease and other dementias, diabetic neuropathy, meningitis, epilepsy, neurological complications due to preterm birth, autism spectrum disorder, and nervous system cancer.InterpretationAs the leading cause of overall disease burden in the world, with increasing global DALY counts, effective prevention, treatment, and rehabilitation strategies for disorders affecting the nervous system are needed

SUPPLEMENTARY EFFECT OF SPIRULINA PLATENSIS ON PERFORMANCE, HEMATOLOGY AND CARCASS YIELD OF BROILER CHICKEN

<p>-A trial was conducted to investigate the effects of dietary supplementations of Spirulina<br>platensis on broiler performance, Hematology and organs weights. Two hundred 1-d-old Broiler chicks<br>were randomly assigned to 1 of 4 dietary treatments, consisting of 2 replicates of 25 birds each.<br>Commercial mash starter and finisher diets were supplemented with 0.5%, 1%, and 1.5% of Spirulina<br>platensis. The Body Weights, Feed Conversion Rate, Hematology, Carcass yield percentage were<br>significantly increased by the dietary inclusion of the 1% of Spirulina platensis as compared the control<br>fed broilers. In conclusion, 1% of Spirulina platensis supplementation significantly increased Body<br>weight, decreased Feed Consumption Ratios and improves the Blood Parameters. The Spirulina<br>platensis offers a good alternative to improve poultry production.</p> <p> </p

Elucidation of neuroprotective role of endogenous GABA and energy metabolites in middle cerebral artery occluded model in rats

391-397The excitatory amino acids (EAA) like glutamate, aspartate and inhibitory neurotransmitter GABA (gama amino butyric acid) play an important role in the pathophysiology of cerebral ischemia. The objective of the present study is to elucidate the role of endogenous GABA against EAA release in different regions during ischemia. The transient focal ischemia was induced in rats by using middle cerebral artery occlusion model (MCAo). The results indicate gradual elevation of brain glutamate, aspartate and GABA level at different brain regions and attained peak level at 72 h of ischemic reperfusion (IR). At 168 h of IR the EAA levels declined to base line but GABA level was found to be still elevated. The biochemical analysis shows the depleted brain ATP, Na+K+ATPase content and triphasic response of glutathione activity. It can be concluded that time dependent variation in the EAA and GABA release, endogenous GABA can be neuroprotective and earlier restoration of energy deprivation is essential to prevent further neurodegeneration. To have efficient treatment in ischemic condition, multiple approaches like energy supply, antagonism of EAA, controlling calcium function are essential.</span