Some issues of regional development and planning in Libya

Thesis (M.A.)--Boston UniversityThis thesis is primarily concerned with the issues of regional development as they relate to a classic problem of underdevelopment, namely, the dualism of the space economy. The reversal of regional imbalance becomes a complex problem because the polarizing process is historically conditioned and is deeply ingrained into the structural economy. Accordingly, the present study of regional development in Libya started with a historical analysis of the space economy. Understanding of the historical evolution of regional economic relations is essential for the contemporary purposes of formulating development plans for modern Libya. Against a painful background of poverty resulting from historical influences and geographical constraints, the Libyan economy has experienced the "oil boom" of the Sixties. Since then, Libya has been characterized by a large capital surplus, as it became one of the major oil producing and exporting nations of the world. However, despite the abundance of capital the economy still reflects the classic structural traits of underdevelopment, i.e., sectoral imbalance and overdependence on a single product. While the oil industry has been developed extensively, other sectors of the economy remained comparatively less developed. In addition to that, the new oil wealth initiated the following problems: 1) the extension of a modern market economy into the traditional subsistence economy, disrupting the life-styles and the population base of the latter; 2) a widening gap between the rapidly growing modern cities and the stagnation or slow growth in rural areas; and 3) increased rural-to-urban migration. Given the fact that oil is an extractive economy of a nonrenewable resource, this research stresses the need for regional population policies that would link the exploitation of oil with areally dispersed industrial and agricultural activities. In this respect, population data from 1954 to 1973 were analyzed in order to identify the growing and declining regions. This analysis was supplemented by a spatial analysis of hierarchical service-centers in order to identify the gaps in space and in the hierarchical order. The results of this investigation were used to suggest a settlement policy for northeastern Libya

Molecular characterization of Pseudomonas aeruginosa isolates from various clinical specimens in Khartoum/Sudan: Antimicrobial resistance and virulence genes

Background: Pseudomonas aeruginosa is a pathogenic organism responsible for frequent wound and nosocomial infections worldwide. Its infections are difficult to control since the organism is known to rapidly develop antibiotic resistance and becomes multidrug-resistant (MDR) during treatment of patients. Aim of the study: This study was intended to investigate the occurrence of certain important types of (ESBL) and (MBL) enzymes in association with important specific virulence factors  associated with P. aeruginosa clinical isolates from Khartoum, Sudan. Methods: This study investigated 70 P. aeruginosa isolates which were collected from patients admitted to four major hospitals in Khartoum  (Fedail, Ribat, Ibn Sina and Soba hospitals). These isolates were recovered from 40 wound swabs (57.1%), 27 urine samples (38.6%), and 3 pleural fluid samples (4.3%) of patients. Higher numbers of isolates were recovered from males 42 (60%) than in females 28 (40%). All P. aeruginosa isolates were first confirmed by conventional biochemical and second using molecular PCR tests.   PCR methods were also used for detecting the presence of the virulence genes ToxA, AlgD, LasB, exoS, exoU, CTX, GES-1, and genes of VIM, IMP, KPC, CTX, VEB-1 and SHV-1. Results:   Antimicrobial susceptibility testing of P. aeruginosa isolates showed a high resistance to azetronam 49 (70%), followed by ceftazidime 32 (45.7%), 16 ciprofloxacin (22.9%), gentamicin 13 (18.6 %), piperacillin-tazobactam 11 (15.7%), amikacin 9 (12.9 %), and imipenem 6 (8.6%) showed the least resistance. All isolates were positive for algD and lasB (100%), followed by toxA (90%), exoS (34.3), exoU (24.3%), respectively. The rates of detected ESBL genes blaTEM, blaCTX-m, blaSHV-1,GES-1, were 3.3%, 6.6%, 10%, 3.3%,10%, respectively, but all isolates were negative for bla-KPC and bla- VIM and IMP . The percentages of pigment production were 61.4% for pyocyanin, 37.1% for pyoverdin and 1.4% for pyorubin. Conclusion: The study demonstrated high rates of antimicrobial resistance markers to most commonly used antibiotics in treatment of P. aeruginosa infections. The majority of the isolates from urine and wound samples carried at least three potential virulence factor genes of algD, lasB and toxA and without any significant relation to their antimicrobial resistance markers. &nbsp

A novel role in cytokinesis reveals a housekeeping function for the unfolded protein response

The unfolded protein response (UPR) pathway helps cells cope with endoplasmic reticulum (ER) stress by activating genes that increase the ER's functional capabilities. We have identified a novel role for the UPR pathway in facilitating budding yeast cytokinesis. Although other cell cycle events are unaffected by conditions that disrupt ER function, cytokinesis is sensitive to these conditions. Moreover, efficient cytokinesis requires the UPR pathway even during unstressed growth conditions. UPR-deficient cells are defective in cytokinesis, and cytokinesis mutants activate the UPR. The UPR likely achieves its role in cytokinesis by sensing small changes in ER load and making according changes in ER capacity. We propose that cytokinesis is one of many cellular events that require a subtle increase in ER function and that the UPR pathway has a previously uncharacterized housekeeping role in maintaining ER plasticity during normal cell growth

Alternative Splicing Regulates Targeting of Malate Dehydrogenase in Yarrowia lipolytica

Alternative pre-mRNA splicing is a major mechanism contributing to the proteome complexity of most eukaryotes, especially mammals. In less complex organisms, such as yeasts, the numbers of genes that contain introns are low and cases of alternative splicing (AS) with functional implications are rare. We report the first case of AS with functional consequences in the yeast Yarrowia lipolytica. The splicing pattern was found to govern the cellular localization of malate dehydrogenase, an enzyme of the central carbon metabolism. This ubiquitous enzyme is involved in the tricarboxylic acid cycle in mitochondria and in the glyoxylate cycle, which takes place in peroxisomes and the cytosol. In Saccharomyces cerevisiae, three genes encode three compartment-specific enzymes. In contrast, only two genes exist in Y. lipolytica. One gene (YlMDH1, YALI0D16753g) encodes a predicted mitochondrial protein, whereas the second gene (YlMDH2, YALI0E14190g) generates the cytosolic and peroxisomal forms through the alternative use of two 3′-splice sites in the second intron. Both splicing variants were detected in cDNA libraries obtained from cells grown under different conditions. Mutants expressing the individual YlMdh2p isoforms tagged with fluorescent proteins confirmed that they localized to either the cytosolic or the peroxisomal compartment

URBAN NETWORKS IN EASTERN 'ABBASID LANDS: AN HISTORICAL GEOGRAPHY OF SETTLEMENT IN MESOPOTAMIA AND PERSIA, NINTH- AND TENTH-CENTURY A.D.

This dissertation explores the application of spatial organization models to medieval Islamic urbanism. In particular, the systems of urban settlements in Mesopotamia and Persia during the ninth and tenth centuries A.D. are investigated, depending primarily on medieval indigenous sources. The study of Islamic urbanism in general, and medieval Islamic urbanism in particular, remained for a long time obscured by an inadequate single perspective: the "Islamic city" as an individual social entity occupying a fixed geographical area. The conventional approach can be criticized for its restricted focus on Islamic cultural tradition as the only explanatory variable and for its search for an ideal-type construct in the tradition of Western urban-ecological writings of the first half of the twentieth century. The alternative approach put forward in the present thesis examines the city as part of a larger urban network extending over several regions. It is argued that the application of spatial organization models to medieval Islamic urbanism will help to clarify the place and role of cities in both the regional and national structures and will provide a suitable framework for comparing the stages of urban and regional development. Following a historical perspective, the study results indicate the sequence in the evolution of a distinctive form of Islamic urbanism through the operation of several spatial processes. Such processes signify the expansion, assimilation, and integration of urban settlements in former Sasanian lands. Analysis of the road network provides the necessary framework by which interurban contacts are examined on both the national and the regional levels. Hierarchical organization of space and settlement interdependencies are further demonstrated by the analysis of long-distance kharaj (land tax) mobility. This medieval fiscal system is used as a surrogate for human spatial interaction and is supplemented by an evidence for the existence of an urban hierarchy derived from the actual methods and approaches used by the medieval Arab geographers themselves. The findings of the present study demonstrate the evidence for the evolution first of a nationally integrated urban system and second of several regionally organized urban subsystems