Regulation of desmocollin transcription in mouse preimplantation embryos

The molecular mechanisms regulating the biogenesis of the first desmosomes to form during mouse embryogenesis have been studied. A sensitive modification of a reverse transcriptase-cDNA amplification procedure has been used to detect transcripts of the desmosomal adhesive cadherin, desmocollin. Sequencing of cDNA amplification products confirmed that two splice variants, a and b, of the DSC2 gene are transcribed coordinately. Transcripts were identified in unfertilized eggs and cumulus cells and in cleavage stages up to the early 8-cell stage, were never detected in compact 8-cell embryos, but were evident again either from the 16-cell morula or very early blastocyst (approx 32-cells) stages onwards. These two phases of transcript detection indicate DSC2 is encoded by maternal and embryonic genomes. Previously, we have shown that desmocollin protein synthesis is undetectable in eggs and cleavage stages but initiates at the early blastocyst stage when desmocollin localises at, and appears to regulate assembly of, nascent desmosomes that form in the trophectoderm but not in the inner cell mass (Fleming, T. P., Garrod, D. R. and Elsmore, A. J. (1991), Development 112, 527–539). Maternal DSC2 mRNA is therefore not translated and presumably is inherited by blastomeres before complete degradation. Our results suggest, however, that initiation of embryonic DSC2 transcription regulates desmocollin protein expression and thereby desmosome formation. Moreover, data from blastocyst single cell analyses suggest that embryonic DSC2 transcription is specific to the trophectoderm lineage. Inhibition of E-cadherin-mediated cell-cell adhesion did not influence the timing of DSC2 embryonic transcription and protein expression. However, isolation and culture of inner cell masses induced an increase in the amount of DSC2 mRNA and protein detected. Taken together, these results suggest that the presence of a contact-free cell surface activates DSC2 transcription in the mouse early embryo. <br/

Critical properties of the Fermi-Bose Kondo and pseudogap Kondo models: Renormalized perturbation theory

Magnetic impurities coupled to both fermionic and bosonic baths or to a fermionic bath with pseudogap density of states, described by the Fermi-Bose Kondo and pseudogap Kondo models, display non-trivial intermediate coupling fixed points associated with critical local-moment fluctuations and local non-Fermi liquid behavior. Based on renormalization group together with a renormalized perturbation expansion around the free-impurity limit, we calculate various impurity properties in the vicinity of those intermediate-coupling fixed points. In particular, we compute the conduction electron T matrix, the impurity susceptibility, and the residual impurity entropy, and relate our findings to certain scenarios of local quantum criticality in strongly correlated lattice models.Comment: 16 pages, 5 figs; (v2) large-N results for entropy of Bose-Kondo model added; (v3) final version as publishe

Composição química, fracionamento de carboidratos e proteínas e digestibilidade in vitro de forrageiras tropicais em diferentes idades de corte

Objetivou-se avaliar o valor nutritivo de três espécies forrageiras tropicais: capim-tanzânia (Panicum maximum Jacq.), capim-marandu (Brachiaria brizantha) e capim-tifton 85 (Cynodon spp), em duas épocas do ano (janeiro-março e abril-junho) e em três idades de rebrota (28, 35 e 42 dias), por meio da composição química, do fracionamento de proteínas e carboidratos e da digestibilidade in vitro da matéria seca (DIVMS) e da matéria orgânica (DIVMO). O capim-marandu destacou-se no período de janeiro-março, com menores conteúdos de parede celular e fração B2 dos carboidratos e maiores valores de proteína bruta, fração A + B1, DIVMS e DIVMO, em comparação aos capins tanzânia e tifton 85, independentemente da idade de corte. O aumento da concentração de parede celular em detrimento ao conteúdo celular com o avanço da maturidade das plantas foi evidente no capim-marandu no período de janeiro-março, quando foram observados maior valor da fração B2, maior conteúdo de fibra em detergente neutro (FDN) e menor concentração da fração carboidratos não-fibrosos. No período de abril-junho, a composição em parede celular não apresentou diferenças evidentes com aumento da idade, devido às condições ambientais observadas. O capim-tanzânia apresenta, de modo geral, baixos valores de parede celular e altos valores de carboidratos não-fibrosos, DIVMS e DIVMO nesse período, seguido pelos capins marandu e tifton 85, respectivamente.This trial was conducted with the objective of evaluating the nutritive value of three tropical forage species: tanzania grass (Panicum maximum Jacq.), marandu grass (Brachiaria brizantha) and Tifton 85 bermudagrass (Cynodon spp) in two different periods of the year (January-March and April-June) and in three cutting ages (28, 35 and 42 days), based on the chemical composition, protein and carbohydrate fractions, and in vitro digestibility of dry matter (DMD) and organic matter (OMD). Marandu grass, in the period January-March, had the lowest cellular wall contents and B2 fraction of carbohydrates, and higher values of crude protein, A + B1 fraction, DMD and OMD in comparison to tanzania grass and Tifton 85 bermudagrass, regardless of the cutting age. The increase of concentration of the cell wall in detriment to the cell content as plant maturity advanced was evident in marandu grass during January-March when higher values of B2 fraction, higher content of neutral detergent fiber and lower concentrations of non fibrous carbohydrate fractions were observed. In April-June period, the cellular wall contents did not show evident differences as age increased due to environmental conditions. The tanzania grass presents, in general, lower values of the cell wall and higher values of non fibrous carbohydrate, DMD and OMD during this period, followed by marandu grass and Tifton 85 bermudagrass, respectively