Three deeply divided lineages of the freshwater mussel genus Anodonta in western North America

Abstract The surprising diversity and recent dramatic decline of freshwater mussels in North America have been well documented, although inventory efforts to date have been concentrated in the eastern United States. Unlike their eastern counterparts, western freshwater mussels have received comparatively little attention. The accurate identity of western lineages is a necessary component for future inventory, monitoring, and ecological work involving these taxa. Here we initiate a study involving the most speciose genus (Anodonta) in western North America, incorporating information about type localities and type specimen morphology and describing the discovery of three highly divergent lineages among four western Anodonta species. In a limited phylogenetic analysis, we find (1) that A. californiensis/nuttalliana and A.oregonensis/kennerlyi are distinct, highly divergent clades, and (2) that A. beringiana is more closely allied with A. woodiana, an Asian species, than either of the other two western North American clades. We were largely unable to resolve the placement of these three clades with respect to other anodontines, and suggest the need for a broader phylogenetic framework. We recommend, however, that the existence of these three deeply divergent groups be considered in the development of regional monitoring, conservation and research plans despite the taxonomic uncertainty

Fish and mussels: importance of fish for freshwater mussel conservation

Co-extinctions are increasingly recognized as one of the major processes leading to the global biodiversity crisis, but there is still limited scientific evidence on the magnitude of potential impacts and causal mechanisms responsible for the decline of affiliate (dependent) species. Freshwater mussels (Bivalvia, Unionida), one of the most threatened faunal groups on Earth, need to pass through a parasitic larval (glochidia) phase using fishes as hosts to complete their life cycle. Here, we provide a synthesis of published evidence on the fish–mussel relationship to explore possible patterns in co-extinction risk and discuss the main threats affecting this interaction. We retrieved 205 publications until December 2015, most of which were performed in North America, completed under laboratory conditions and were aimed at characterizing the life cycle and/or determining the suitable fish hosts for freshwater mussels. Mussel species were reported to infest between one and 53 fish species, with some fish families (e.g., Cyprinidae and Percidae) being used more often as hosts than others. No relationship was found between the breadth of host use and the extinction risk of freshwater mussels. Very few studies focused on threats affecting the fish–mussel relationship, a knowledge gap that may impair the application of future conservation measures. Here, we identify a variety of threats that may negatively affect fish species, document and discuss the concomitant impacts on freshwater mussels, and suggest directions for future studies.The Portuguese Foundation for Science and Technology—FCT through POPH/FSE funds supported VM, MI and MLL under grants (SFRH/BD/108298/2015), (SFRH/BPD/90088/2012), (SFRH/BD/115728/2016), respectively. KD acknowledges the support from the Czech Science Foundation (13-05872S). RS acknowledges the support of the strategic programme UID/BIA/04050/2013 (POCI-01-0145- FEDER-007569) funded by national funds through the FCT I.P. and by the ERDF through the COMPETE2020-Programa Operacional Competitividade e Internacionalização (POCI). This study was conducted as part of the project FRESHCO: Multiple implications of invasive species on Freshwater Mussel co-extinction processes, supported by FCT (contract: PTDC/AGRFOR/1627/2014)

Freshwater Mussels of the Chattahoochee and Flint Rivers

Proceedings of the 1993 Georgia Water Resources Conference, April 20-21, 1993, Athens, Georgia.A 1991 and 1992 survey of the freshwater mussels of the Apalachicola, Chattahoochee and Flint (ACF) rivers showed that mussel populations throughout the ACF basin were greatly reduced in diversity and population levels. To protect this diminishing fauna, conservation efforts should focus on ecosystem protection rather than single species recovery.Sponsored and Organized by: U.S. Geological Survey, Georgia Department of Natural Resources, The University of Georgia, Georgia State University, Georgia Institute of TechnologyThis book was published by the Institute of Natural Resources, The University of Georgia, Athens, Georgia 30602 with partial funding provided by the U.S. Department of Interior, Geological Survey, through the Georgia Water Research Institute as authorized by the Water Resources Research Act of 1984 (P.L. 98-242). The views and statements advanced in this publication are solely those of the authors and do not represent official views or policies of the University of Georgia or the U.S. Geological Survey or the conference sponsors

Application of a Dynamic Model to Assess Controls on Age-0 Colorado Pikeminnow Distribution in the Middle Green River, Colorado and Utah

Analysis of field data and development and application of a dynamic model indicate that the processes that control the number and distribution of age-0 Colorado pikeminnow in the middle Green River are poorly understood. Colorado pikeminnow are a federally endangered species endemic to the Colorado River basin that utilize backwaters during their larval stage. The present agency-mandated field sampling program for backwater habitats may be inadequate, because it takes place at a time when the model predicts that most larval fish have drifted beyond the study area. The model predicts that water releases from Flaming Gorge Dam have a large potential effect on larval drift, because high releases at the time of drift greatly increase the proportion of the population transported beyond the study area to unfavorable river environments

Diet of dingoes and cats in central Australia: does trophic competition underpin a rare mammal refuge?

We investigated the hypothesis that trophic competition between a top predator and a smaller predator can create refuge from predation for small mammalian prey, using the dingo (Canis lupus dingo) and feral cat (Felis catus) in the MacDonnell Ranges of dryland Australia as a case study. We analyzed the diets of the 2 predator species for evidence of potential competition. There was no evidence of exploitation competition between the 2 carnivores—cats consumed mostly small mammals and particularly larger rodents, whereas the diet of dingoes was dominated by 1 species of large macropod. There was also no evidence of a shift in diet of cats, as their diets in refuges and non-refuges were highly overlapping. Consistent with interference competition, cats were the third most frequently consumed mammal species by dingoes. Although predation by dingoes could limit densities of cats across the MacDonnell Ranges, this alone does not explain why the most rugged habitats in the region are a refuge for rare mammals. We conclude that habitat complexity most likely underpins the refuge and that possible effects of dingo predation on the cat population would be of secondary importance

Diet of dingoes and cats in central Australia: does trophic competition underpin a rare mammal refuge?

We investigated the hypothesis that trophic competition between a top predator and a smaller predator can create refuge from predation for small mammalian prey, using the dingo (Canis lupus dingo) and feral cat (Felis catus) in the MacDonnell Ranges of dryland Australia as a case study. We analyzed the diets of the 2 predator species for evidence of potential competition. There was no evidence of exploitation competition between the 2 carnivores—cats consumed mostly small mammals and particularly larger rodents, whereas the diet of dingoes was dominated by 1 species of large macropod. There was also no evidence of a shift in diet of cats, as their diets in refuges and non-refuges were highly overlapping. Consistent with interference competition, cats were the third most frequently consumed mammal species by dingoes. Although predation by dingoes could limit densities of cats across the MacDonnell Ranges, this alone does not explain why the most rugged habitats in the region are a refuge for rare mammals. We conclude that habitat complexity most likely underpins the refuge and that possible effects of dingo predation on the cat population would be of secondary importance

The impacts of an invasive herbivore (Camelus dromedaries) on arid zone freshwater pools: An experimental investigation of the effects of dung on macroinvertebrate colonisation

Aquatic ecosystems in arid environments provide important refugia and ‘stepping-stones’ of connectivity for aquatic fauna. Aquatic ecosystems in central Australia are vulnerable to degradation due to the impacts of invasive herbivores such as camels, which degrade small desert waterbodies through drinking, trampling, and fouling with dung. In this study we assessed the impacts of camel dung on the water quality and macroinvertebrate colonization and community composition of small arid zone freshwater pools using experimental mesocosms.Camel dung (2 kg) was added to half the mesocosms (the treatment), the remaining mesocosms (without camel dung) acted as the controls. All mesocosms were sampled weekly for water quality, nutrients, chlorophyll a and macroinvertebrate richness and abundance, over an eight week period during summer.Macroinvertebrate abundance was higher in the control mesocosms in comparison to the treatment mesocosms. Pollution tolerant taxa such as mosquito larvae were common in treatment mesocosms, while sensitive fauna, such as larval mayflies and dragonflies were more common in the controls. The latter are predators and appeared to have a major influence on community composition.Our results reinforce the need for active management of invasive herbivores to protect aquatic biodiversity and to manage potential disease-vector species in central Australia waterbodies

Isolation and Characterization of Microsatellite Loci in the Western Pearlshell Mussel, \u3ci\u3eMargaritifera falcata\u3c/i\u3e (Gould)

Ten microsatellite loci were isolated from the western pearlshell, Margaritifera falcata (Gould, 1850) and characterized in populations from Washington and Montana, USA. We also assessed eight microsatellite loci developed in M. margaritifera, two of which showed utility. Both of our test populations showed significant heterozygote deficiencies at most loci, consistent with a hermaphroditic life history. Populations differed markedly with respect to allelic richness, allele frequencies and numbers of identical multilocus genotypes. This panel of loci should prove useful in describing gene flow and genetic diversity patterns among M. falcata populations, information that should aid future conservation efforts