Caracterización química del aceite de barú y su subproducto de la región noroeste de Minas Gerais, Brasil

This study investigated baru oil and partially defatted baru flour from the northwest region of Minas Gerais, Brazil. The physicochemical characterization of the oil was made by determining the fatty acid profile using gas chromatography, lutein, and α- and β- carotenes by means of high-performance liquid chromatography, and total carotenoids by spectrophotometry. The flour was analyzed for its chemical composition, fiber, and mineral contents. Baru oil presented excellent quality parameters and high contents in unsaturated fatty acids and carotenoids. The flour showed relevant levels of proteins, lipids, and dietary fiber, in addition to having representative mineral contents for food such as manganese, magnesium, and copper. Thus, baru oil and the by-product of its extraction offer a rich chemical composition, and their application may add nutritional value to foods in addition to reducing negative environmental impacts.En este estudio se investigó el aceite de barú y la harina de barú parcialmente desengrasada de la región noroeste de Minas Gerais, Brasil. La caracterización físico-química del aceite se realizó mediante la determinación del perfil de ácidos grasos mediante cromatografía de gases, luteína y α- y β- carotenos mediante cromatografía líquida de alta resolución y carotenoides totales mediante espectrofotometría. En la harina se analizó su composición química, fibra y contenido mineral. El aceite de barú tiene excelentes parámetros de calidad, un buen contenido de ácidos grasos insaturados y carotenoides. La harina presentó niveles relevantes de proteínas, lípidos y fibra dietética, además de tener un contenido representativo de minerales para la alimentación, como manganeso, magnesio y cobre. Así, el aceite de baru y el subproducto de su extracción tienen riqueza en su composición química y su aplicación puede agregar valor nutricional a los alimentos, además de reducir los impactos ambientales

ELISA and modified toxin-binding inhibition test for quality control of the clostridial vaccine processes

This study aimed to assess and standardize the ELISA and modified ToBI test in vitro methods in order to verify the potency of epsilon toxicoid in comparison with the in vivo TCP method. The following epsilon toxoids were used: NIBSC standard from batches 375/07, 532/08, 551/08, 373/07 and 378/07. These were evaluated using a TCP test, ELISA and ToBI tests. The results indicate that the correlation ratio between the dilutions of standard NIBSC toxicoid and absorbance values of 89.44% obtained with the ELISA method support the use of the curve to evaluate epsilon toxoids. However, it was observed that the absorbance values were similar for all toxoids, thus presenting no significant difference between higher and lower concentration toxoids. For the ToBI test, the correlation ratio of 96.76, obtained in the curve pattern, demonstrates the effectiveness of the curve to be used in the epsilon toxoid evaluation. The correlation ratio between the titration degrees of toxoids obtained through TCP and ToBI tests was higher than 90%. It is concluded that the type of ELISA test used does present discriminative power for toxoids with different concentrations, which does not support the use of this technique for such a purpose. The ToBI test can be used as a screening method for it is sensitive and effective to detect epsilon toxicoid produced by C. perfringens type D

Recovery of alpha-toxin from Clostridium perfringens type A with an aqueous two-phase system in a Perforated Rotating Disc Contactor

The influence of dispersed phase flowrate and disc rotational speed on mass transfer coefficient of alpha-toxin from Clostridium perfrigens type A and purification factor was investigated in this work