SOLUÇÃO ESTÉTICA PARA ABRASÕES CERVICAIS PROFUNDAS RESTAURADAS COM MATERIAL RESINOSO ASSOCIADA AO RECOBRIMENTO RADICULAR: RELATO DE CASO CLÍNICO

O uso de materiais restauradores resinosos tem-se mostrado uma alternativa biocompatível na restauração de abrasões cervicais e/ ou cáries profundas previamente ao procedimento cirúrgico de recobrimento radicular. Este trabalho tem como objetivo mostrar os resultados obtidos em um caso clínico onde abrasões cervicais profundas foram previamente restauradas ao procedimento cirúrgico de recobrimento radicular. Paciente do sexo masculino, 56 anos, compareceu à Clínica de Periodontia do Unicenp, queixando-se de falta de estética na região dos elementos dentários 14, 15 e 16. Por meio do exame clínico, detectou-se a presença de recessões gengivais múltiplas, classe II de Miller, associadas com abrasões cervicais profundas. Além da realização da terapia básica periodontal, incluindo a orientação de higiene bucal, realizou-se a restauração das abrasões cervicais, com resina composta microhíbrida, 2 semanas antes da técnica cirúrgica de recobrimento radicular com enxerto de tecido conjuntivo subepitelial. Após 6 meses, observou-se recobrimento parcial das superfícies radiculares anteriormente expostas e saúde periodontal, além da total satisfação do paciente. A associação entre tratamento restaurador e recobrimento radicular para tratar abrasões cervicais profundas pode ser considerada uma técnica de sucesso

Experimental periodontal disease treatment by subgingival irrigation with tetracycline hydrochloride in rats

OBJECTIVE: The aim of this study was to compare subgingival irrigation with tetracycline hydrochloride (TTC-HCL) as adjunctive treatment to scaling and root planning (SRP) on induced periodontitis in rats. MATERIAL AND METHODS: In 60 rats, periodontal disease was ligature-induced at the mandibular left first molar. After 7 days, the ligature was removed and all animals were submitted to SRP, and divided into 2 groups according to the following treatment: C (n=30) - subgingival irrigation with 1 mL of saline; T (n=30) - subgingival irrigation with 1 mL of TTC-HCL (50 mg/mL). Ten animals in each group were euthanized at 7, 15 and 30 days posttreatment. The histometric values were statistically analyzed (

Influence of nicotine on healing process of autogenous bone block grafts in the mandible: A histomorphometric study in rats

Purpose: The aim of this study was to perform qualitative and quantitative analyses of the effect of nicotine on autogenous bone block grafts and to describe events in the initial healing phase and the differences in the repair processes between animals exposed to nicotine and controls. Materials and Methods: Forty-eight female Wistar rats were randomly divided into 2 groups, the nicotine group and the saline group. All animals received either nicotine (3 mg/kg) or saline 4 weeks before the surgical procedure and continued to receive nicotine from surgery to sacrifice at 7, 14, or 28 days. The autogenous bone block graft was harvested from the calvaria and stabilized on the external cortical area near the angle of the mandible. Results: The histologic analyses of the nicotine group depicted a delay in osteogenic activity at the bed-graft interface, as well as impairment of the organization of the granulation tissue that developed instead of blood clot. Nicotine-group specimens exhibited less bone neoformation, and the newly formed bone was poorly cellularized and vascularized. The histometric analysis revealed significantly less bone formation in the nicotine group at both 14 days (23.75% +/- 6.18% versus 51.31% +/- 8.31%) and 28 days (42.44% +/- 8.70% versus 73.00% +/- 4.99%). Conclusion: Nicotine did jeopardize the early healing process of autogenous bone block grafts in rats but did not prevent it

Lesão endodôntica com manifestação periodontal. Relato de caso.

Lesão endodôntica com manifestação periodontal. Relato de caso

Effects of oestrogen deficiency and 17 beta-estradiol therapy on bone healing in calvarial critical size defects treated with bovine bone graft

Objective: To histomorphometrically analyze the effect of ovariectomy-induced oestrogen deficiency and 17 beta-estradiol therapy on bone healing of surgically created critical-size defects (CSDs) treated with bovine bone graft (BBG).Methods: Forty-eight female rats were randomly assigned to the following 3 experimental groups (n = 16): sham-operated animals (SHAM), ovariectomized animals (OVX) and ovariectomized animals treated with oestrogen (OVX+E2). OVX+E2 animals received daily subcutaneous injections of 17 beta-estradiol (20 mu g/kg) from 8 days after ovariectomy until euthanasia. Thirty days after the surgery, an 8 mm CSD was surgically created in each calvaria of all animals and filled with BBG. Animals were euthanized at either 30 or 60 days postoperative. A histological analysis, percentage of Newly Formed Bone Area (NFBA), osteoblast and osteoclast number was histomorphometrically performed (p <= 0.05).Results: At 30 days, SHAM group (8.82% +/- 2.93) had significantly greater NFBA than OVX (4.66% +/- 1.35) and OVX+E2 groups (5.85% +/- 4.08) (p <= 0.05). At 60 days, SHAM group (11.51% +/- 3.08) and OVX+E2 group (9.84% +/- 1.87) had significantly greater NFBA than OVX animals (5.12% +/- 0.68) (p <= 0.05). Fewer osteoblasts were observed in the OVX group at 30 (763.40 +/- 121.60) and 60 (696.60 +/- 80.92) days than in the SHAM group at 30 days (1356.00 +/- 95.38). Fewer osteoclasts were observed in the OVX+E2 group (3.25 +/- 2.16) than in the SHAM (9.75 +/- 1.82) and OVX (12.75 +/- 1.47) groups at 30 days (p <= 0.05).Conclusions: Oestrogen deficiency compromises bone healing in calvarial CSDs treated with BBG in ovariectomized rats. After 60 days post-surgery, 17 beta-estradiol therapy improved bone healing in calvarial CSDs treated with BBG in ovariectomized rats.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

A standartized research protocol for platelet-rich plasma (PRP) preparation in rats

Introduction: The urgent need for studies using standardized protocols to evaluate the real biological effects of PRP has been emphasized by several authors. Objective: The purpose of this study was to standardize a methodology for autologous Platelet-Rich Plasma (PRP) preparation in rats. Material and methods: Twentyfour, 5 to 6-month-old, male rats, weighing 450 to 500 g were used. After general anesthesia, 3.15 ml of blood was collected from each animal, via cannulation of the jugular vein. A standardized technique of double centrifugation was used to prepare PRP. PRP samples and peripheral blood platelets were then manually counted using a Neubauer chamber. Student’s t-test was used to compare the differences between the number of platelets in peripheral blood and PRP samples (p < 0.05). In addition, PRP and peripheral blood smears were stained to see platelets’ morphology. Results: All surgical procedures were well tolerated by the animals and they were healthy during the entire experimental period. PRP samples showed higher significantly platelet concentrations than peripheral blood samples (2,677,583 and 683,680 respectively). Conclusion: Within the limits of this study, it can be concluded that the method used produced autologous PRP with appropriated platelet quantity and quality, in rats

Oral focal mucinosis: clinical case presentation with a focus on aesthetic and functional surgical resolution

Introduction: When planning excisional surgery, alterations in the oral mucosa, particularly in esthetically important areas, represent a challenge. For cases involving an exophytic lesion with a vegetative tumoral aspect, a clinician may prioritize obtaining a definitive diagnosis by histopathologic and histochemical examinations over the preservation of esthetics or maintenance of the involved tooth. Case Presentation: A painless, hard lesion with normal coloration was present in the maxillary left central incisor of a patient for at least 6 years. A biopsy was performed to preserve oral mucosa and a treatment plan was established that included endodontic retreatment and prosthetic restoration. The histopathologic diagnosis was oral focal mucinosis, an uncommon and poorly characterized type of lesion. Conclusion: A conservative biopsy of the lesion facilitated an accurate diagnosis, promoted the patient’s comfort, and achieved satisfactory esthetic results