DEFINITION OF PRESSURE INTRAMYOCARDIAL AS A METHOD FOR EVALUATING MORPHOFUNCTIONAL STATE STRUCTURE OF THE HEART AT DIFFERENT STAGES OF ONTOGENESIS

Intramyocardial pressure structures of the heart is determined by qualitative and. quantita-tive characteristics of its constituent structural elements. Correlation analysis revealed that the formation of stress-strain states in addition to cardiomyocytes affect fibers of connective tissue

Morphological estimation of changes in cancellous raduis bone for forensic age determination

Visual researches showed that the cancellous bone of the distal radius has significant morphological changes with the increase of years. Revealed structural changes of cancellous bone allows to differentiate the age of an object and to direct a medical expert towards the proposed age group at the identification study. As a result of photocolorimetric research of involution changes of cancellous bone of the distal radius we found statistically significant increase of light transmission with the increase of years which is associated with an increase of osteoporosis. The correlation coefficient ranged from 0,63 to 0,80. On the basis of these data mathematical models that allow to determine the person's age in the forensic identification researches of the distal radius were developed (standard mean square error in determination of the age was from 6,6 to 8,2 years)

Unresolved Multiple Stars and Galactic Clusters' Mass Estimates

If not properly accounted for, unresolved binary stars can induce a bias in the photometric determination of star cluster masses inferred from star counts and the luminosity function. A correction factor close to 1.15 (for a binary fraction of 0.35) was found in Borodina et al., which needs to be applied to blind photometric mass estimates. This value for the correction factor was found to be smaller than literature values. In an attempt to lift this discrepancy, in this work the focus is on higher order multiple stars with the goal of investigating the effect of triple and quadruple systems adopting the same methodology and data set as in the quoted work. The result is that when triple and quadruple, together with binary, systems are properly accounted for, the actual cluster mass (computed as all stars were single) should be incremented by a factor of 1.18-1.27, depending on the cluster and when the binary fraction α is 0.35. Fitting formulae are provided to derive the increment factor for different binary star percentages. © 2021. The American Astronomical Society. All rights reserved.

Regularities of structural-cellular structure of ovaries in ontogenesis

The article presents the examination of the ovaries of embryos, ovaries of human feti and ovaries of children of the tender age. The researches revealed the increase of the surface of follicles, nucleus and primary oocyte's cytoplasm that tells about the fact that further follicular maturation continues in the ovaries of a child of 2-4 months old. Also we registered the increase of nuclear cytoplasmic ratio, appearance of single mature follicles with cumulus oophorus that testifies to the fact that the ovary is functionally mature. Furthermore the research revealed increased content of lipids and DNA in follicular epithelium that also tells aboutfunctional maturity of the ovary. It was found that incretory activity of an ovary during the first year of life is provided by the cells originated from the connective tissue membrane of atresic follicles

A Study of Embryotoxic, Foetotoxic, and Teratogenic Effects of the Original Antituberculosis Agent Thiozonide in Pregnant Rabbits

Several treatment regimens with antituberculosis medicinal products are available for tuberculosis. Thiozonide is a newly developed original antimicrobial agent that exhibits bacteriostatic activity against Mycobacterium tuberculosis strains H37Rv, CN-37, CN-40, and MS-115.The aim of the study was to investigate the embryotoxic, foetotoxic, and teratogenic effects of thiozonide in pregnant rabbits.Materials and methods. The study involved 66 pregnant rabbits (4 groups of 16–17 animals each). The rabbits received oral thiozonide from day 6 to day 19 of gestation at doses of 20.6 mg/kg (1 TD (therapeutic dose)), 103 mg/kg (5 TDs), and 206 mg/kg (10 TDs). The control group received a 1% starch solution. The authors conducted a macroscopic examination of the reproductive organs and a histological evaluation of the placenta in eutha nised pregnant rabbits. Live foetuses underwent a check for developmental abnormalities, a skeletal development evaluation with a modified Dawson’s method, and a histologic examination of the internal organs.Results. The study showed no clinical signs of toxicity and no mortality associated with thiozonide in pregnant rabbits across all dose groups. Macroscopic and histological examinations revealed no pathological changes in the reproductive organs of pregnant rabbits. The evaluation of embryotoxic and foetotoxic effects did not identify any differences between the foetuses of the animals assigned to different doses of thiozonide and the control group. The authors found no developmental abnormalities in the foetuses. Examinations of foetal skeleton development and internal organ condition identified no differences between the groups and no abnormalities. The authors registered the death of all foetuses (late resorption) in one rabbit from the 206 mg/kg group. Therefore, the 103 mg/kg dose (5 TDs) was selected as a reasonable No Observed Adverse Effect Level (NOAEL).Conclusions. Thiozonide has no embryotoxic, foetotoxic, or teratogenic effects

Label-free electrochemical monitoring of DNA ligase activity

This study presents a simple, label-free electrochemical technique for the monitoring of DNA ligase activity. DNA ligases are enzymes that catalyze joining of breaks in the backbone of DNA and are of significant scientific interest due to their essential nature in DNA metabolism and their importance to a range of molecular biological methodologies. The electrochemical behavior of DNA at mercury and some amalgam electrodes is strongly influenced by its backbone structure, allowing a perfect discrimination between DNA molecules containing or lacking free ends. This variation in electrochemical behavior has been utilized previously for a sensitive detection of DNA damage involving the sugar-phosphate backbone breakage. Here we show that the same principle can be utilized for monitoring of a reverse process, i.e., the repair of strand breaks by action of the DNA ligases. We demonstrate applications of the electrochemical technique for a distinction between ligatable and unligatable breaks in plasmid DNA using T4 DNA ligase, as well as for studies of the DNA backbone-joining activity in recombinant fragments of E. coli DNA ligase