Sustentabilidade social em Paty do Alferes - RJ.

bitstream/CNPS/11846/1/bpd02_2002_paty.pd

Percepção ambiental em Paty do Alferes - RJ.

bitstream/CNPS/11844/1/bpd06_2002_paty.pd

Farmer and scientific knowledge of soil quality: a social ecological soil systems approach.

This article focuses on how farmers identify and evaluate the quality of soils cultivated and how their indicators compare to those used by agricultural scientists. The aim is to bridge the gap between specific farmer knowledge and universal scientific knowledge by adopting an ecosystem framework applied to agriculture through the concept of agro-ecosystems. This approach was applied to farming in mountainous areas of the Brazilian Atlantic Forest, a global environmental hotspot that has been degraded over time. In order to reverse this trend, local actors have to build agro-ecological systems that maintain environmental quality, agronomic sustainability and socio-economic viability. For this to happen, local and scientific knowledge must be bridged and mutually adapted in order to be successful. This study therefore concentrates on processes of inter-communication between farmers and agricultural scientists concerning the role of soil quality in farming and conservation

Direcionamento das linhas de plantio em diferentes orientações cardeais e seus reflexos sobre a produtividade de cafeeiros.

Uma variação ambiental que provoca alterações na morfologia externa da planta de café se refere à localização das fileiras do café em relação a orientações dos pontos cardeais. Objetivou-se neste estudo avaliar a produtividade individual de cada lado de plantas de café implantadas em diferentes orientações cardeais buscando subsídios para otimizar o plantio direcionado e o manejo de lavoura em solo com topografia plana a suave ondulada. Observa-se de maneira geral que, independentemente do direcionamento das ruas de café em relação as orientações cardeais, a produtividade de café, no lado da planta que recebeu sol pela manhã, foi sempre maior que aquela apresentada pelo lado da planta que recebeu sol à tarde. Estas observações levam a concluir que os melhores direcionamentos de rua de café são no sentido leste-oeste e nordeste-sudoeste e que os dois lados da planta, relacionados com o posicionamento solar, comportam-se como se pertencessem a diferentes lavouras, já que os dois lados da planta apresentam diferenças na morfo-anatomia, na partição de carboidratos, na assimilação do CO2 e nas características hídricas a ponto de alterar o crescimento das plantas e sua produção

Longitudinal metagenomic profiling of bovine milk to assess the impact of intramammary treatment using a third-generation cephalosporin

Antimicrobial usage in food animals has a direct impact on human health, and approximately 80% of the antibiotics prescribed in the dairy industry are used to treat bovine mastitis. Here we provide a longitudinal description of the changes in the microbiome of milk that are associated with mastitis and antimicrobial therapy. Next-generation sequencing, 16 S rRNA gene quantitative real-time PCR, and aerobic culturing were applied to assess the effect of disease and antibiotic therapy on the milk microbiome. Cows diagnosed with clinical mastitis associated with Gram-negative pathogens or negative aerobic culture were randomly allocated into 5 days of Ceftiofur intramammary treatment or remained as untreated controls. Serial milk samples were collected from the affected quarter and the ipsilateral healthy quarter of the same animal. Milk from the mastitic quarter had a higher bacterial load and reduced microbial diversity compared to healthy milk. Resolution of the disease was accompanied by increases in diversity indexes and a decrease in pathogen relative abundance. Escherichia coli-associated mastitic milk samples had a remarkably distinct bacterial profile, dominated by Enterobacteriaceae, when compared to healthy milk. However, no differences were observed in culture-negative mastitis samples when compared to healthy milk. Antimicrobial treatment had no significant effect on clinical cure, bacteriological cure, pathogen clearance rate or bacterial load

The bovine milk microbiota: insights and perspectives from -omics studies

Recent significant progress in culture-independent techniques, together with the parallel development of -omics technologies and data analysis capabilities, have led to a new perception of the milk microbiota as a complex microbial community with great diversity and multifaceted biological roles, living in an environment that was until recently believed to be sterile. In this review, we summarize and discuss the latest findings on the milk microbiota in dairy cows, with a focus on the role it plays in bovine physiology and health. Following an introduction on microbial communities and the importance of their study, we present an overview of the -omics methods currently available for their characterization, and outline the potential offered by a systems biology approach encompassing metatranscriptomics, metaproteomics, and metametabolomics. Then, we review the recent discoveries on the dairy cow milk microbiome enabled by the application of -omics approaches. Learning from studies in humans and in the mouse model, and after a description of the endogenous route hypothesis, we discuss the role of the milk microbiota in the physiology and health of both the mother and the offspring, and report how it can be changed by farming practices and during infection. In conclusion, we shortly outline the impact of the milk microbiota on the quality of milk and of dairy products

16S rRNA amplicon sequencing reveals a polymicrobial nature of complicated claw horn disruption lesions and interdigital phlegmon in dairy cattle

Lameness represents an intractable problem for the dairy industry. Complicated claw horn disruption lesions, interdigital hyperplasia, and interdigital phlegmon are important lameness causing foot lesions. Their aetiology is multifactorial, but infectious processes are likely implicated in disease pathogenesis. Our aim was to investigate the bacterial profiles of these lesions using 16S rRNA gene sequencing of samples obtained from 51 cattle across ten farms in the UK. In this study, interdigital hyperplasia, interdigital hyperplasia with signs of interdigital dermatitis, interdigital phlegmon, complicated sole ulcers, complicated toe ulcers lesions, and complicated white line lesions were investigated; corresponding healthy skin control samples were also analysed. All diseased tissues displayed reduced microbial richness and diversity (as described by Chao1, Shannon, and Simpson alpha-diversity indices) compared to their healthy skin control samples. Our results confirm the association of Treponema spp with some of these disorders. Other anaerobic bacteria including Fusobacterium spp., Fastidiosipila spp. and Porphyromonas spp. were implicated in the aetiology of all these lesions with the exception of interdigital hyperplasia. Complicated claw horn disruption lesions, and interdigital phlegmon were found to have similar bacterial profiles. Such sharing of bacterial genera suggests many of the infectious agents detected in these foot lesions are acting opportunistically; this finding could contribute towards future treatment and control strategies

The bovine foot skin microbiota is associated with host genotype and the development of infectious digital dermatitis lesions

Abstract Background Bovine Digital Dermatitis (BDD) is a prevalent infectious disease, causing painful foot skin lesions and lameness in cattle. We describe herein the bovine foot skin microbiota and its associations with BDD using 16S rRNA gene amplicon and shotgun metagenomic sequencing on samples from 259 dairy cows from three UK dairy farms. Results We show evidence of dysbiosis, and differences in taxonomy and functional profiles in the bovine foot skin microbiome of clinically healthy animals that subsequently develop BDD lesions, compared to those that do not. Our results suggest that taxonomical and functional differences together with alterations in ecological interactions between bacteria in the normal foot skin microbiome may predispose an animal to develop BDD lesions. Using genome-wide association and regional heritability mapping approaches, we provide first evidence for interactions between host genotype and certain members of the foot skin microbiota. We show the existence of significant genetic variation in the relative abundance of Treponema spp. and Peptoclostridium spp. and identify regions in the bovine genome that explain a significant proportion of this variation. Conclusions Collectively this work shows early changes in taxonomic and functional profiles of the bovine foot-skin microbiota in clinically healthy animals which are associated with subsequent development of BDD and could be relevant to prevention of disease. The description of host genetic control of members of the foot skin microbiota, combined with the association of the latter with BDD development offer new insights into a complex relationship that can be exploited in selective breeding programmes. </jats:sec