Worrying about 'vertical landscapes'. Terraced olive groves and ecosystem services in marginal land in central Italy

Terraced Mediterranean areas are distinctive man-made landscapes with historical and cultural relevance. Terraced land abandonment driven by physical and economic constraints had important ecological consequences. This study focuses on a marginal agricultural district in southern Latium, central Italy, where terracing dated back to the Roman period and olive groves are the main agricultural use. A diachronic assessment of land-use transformations was carried out to identify landscape dynamics and drivers of change around terraced land. Terraced landscape systems (TLS), derived from spatial aggregation of neighboring terraced patches, have been analyzed for landscape transformations considering slope as the main stratification variable. Structural and functional characteristics of TLS were analyzed using a landscape ecology approach. Soil bio-chemical indicators were finally assessed to study the impact of terraced olive agro-ecosystems on soil-related ecosystems services. The empirical findings outlined that TLS in central Italy are sensitive to urbanization and land abandonment. Cultivated terraces prevailed up to gentle-medium slope land, uncultivated and wooded areas dominated terraces on steep slopes. In this context, poly-cultural olive groves proved to be a cropping system particularly resilient to global change, irrespective of land slope. Terraced systems and extensive poly-cultural olive groves play a role in preserving ecosystem integrity, landscape quality, soil functionality and, therefore, environmental sustainability

In vitro and in vivo evaluation of T and B lymphocyte functions in AKR mice.

To investigate whether AKR spontaneous leukaemogenesis is associated with a reduction in functional activity of T lymphocytes, the PHA response of AKR blood cells at different ages up to and including the preleukaemic period was studied. No significant differences were observed among young, adult and preleukaemic donors. In addition, the in vitro and in vivo AKR lymphocyte functions were compared with those of CBA lymphocytes by means of their response to stimulation with T and B lymphocyte selective mitogens (PHA, Con A and LSP respectively), and their response to immunization with thymus dependent (SRBC) or independent (LPS) antigens. We observed in vitro that while the B lymphocytes responded normally to mitogen, an intrinsic hyporeactivity to mitogens characterizes the T lymphocytes. Moreover, AKR mice exhibited a reduced in vivo response to both thymus dependent and independent antigens

Luz e temperatura na germinação de sementes de pimenta longa (Piper hispidinervum) e pimenta-de-macaco (Piper aduncum).

As espécies Piper hispidinervum e Piper aduncum destacam-se como produtoras de óleos essenciais ricos em compostos de interesse às indústrias de perfumaria e produtos fitossanitários, como o safrol e dilapiol. Essas espécies encontram-se em processo de domesticação e estudos sobre o processo germinativo de suas sementes são escassos. Assim, objetivou-se com o presente trabalho avaliar a viabilidade de sementes de P. hispidinervum e P. aduncum, buscando determinar um protocolo eficiente para a realização do teste de germinação para cada espécie estudada. O trabalho foi conduzido no período de julho a agosto de 2008, sendo as sementes submetidas a diferentes combinações de temperatura e de luz: 20 °C e 25 °C sob luz branca e difusa; e 20-30 °C alternando escuro (16 h) e claro (8 h), totalizando cinco tratamentos. O tratamento utilizando a temperatura de 25 °C com incidência de luz branca foi o que produziu melhores resultados, com maiores valores de porcentagem e velocidade de germinação sendo, portanto, indicado para avaliação da qualidade fisiológica das sementes de pimenta longa e pimenta-de-macaco

Synthesis and anticancer activity of CDDO and CDDO-me, two derivatives of natural triterpenoids

Triterpenoids are natural compounds synthesized by plants through cyclization of squalene, known for their weak anti-inflammatory activity. 2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oic acid (CDDO), and its C28 modified derivative, methyl-ester (CDDO-Me, also known as bardoxolone methyl), are two synthetic derivatives of oleanolic acid, synthesized more than 20 years ago, in an attempt to enhance the anti-inflammatory behavior of the natural compound. These molecules have been extensively investigated for their strong ability to exert antiproliferative, antiangiogenic, and antimetastatic activities, and to induce apoptosis and differentiation in cancer cells. Here, we discuss the chemical properties of natural triterpenoids, the pathways of synthesis and the biological effects of CDDO and its derivative CDDO-Me. At nanomolar doses, CDDO and CDDO-Me have been shown to protect cells and tissues from oxidative stress by increasing the transcriptional activity of the nuclear factor (erythroid-derived 2)-like 2 (Nrf2). At doses higher than 100 nM, CDDO and CDDO-Me are able to modulate the differentiation of a variety of cell types, both tumor cell lines or primary culture cell, while at micromolar doses these compounds exert an anticancer effect in multiple manners; by inducing extrinsic or intrinsic apoptotic pathways, or autophagic cell death, by inhibiting telomerase activity, by disrupting mitochondrial functions through Lon protease inhibition, and by blocking the deubiquitylating enzyme USP7. CDDO-Me demonstrated its efficacy as anticancer drugs in different mouse models, and versus several types of cancer. Several clinical trials have been started in humans for evaluating CDDO-Me efficacy as anticancer and anti-inflammatory drug; despite promising results, significant increase in heart failure events represented an obstacle for the clinical use of CDDO-Me