Otimização da utilização de marcadores moleculares microssatélites e sua aplicação em estudos com plantas daninhas.

Nota técnica

ATLANTIC EPIPHYTES: a data set of vascular and non-vascular epiphyte plants and lichens from the Atlantic Forest

Epiphytes are hyper-diverse and one of the frequently undervalued life forms in plant surveys and biodiversity inventories. Epiphytes of the Atlantic Forest, one of the most endangered ecosystems in the world, have high endemism and radiated recently in the Pliocene. We aimed to (1) compile an extensive Atlantic Forest data set on vascular, non-vascular plants (including hemiepiphytes), and lichen epiphyte species occurrence and abundance; (2) describe the epiphyte distribution in the Atlantic Forest, in order to indicate future sampling efforts. Our work presents the first epiphyte data set with information on abundance and occurrence of epiphyte phorophyte species. All data compiled here come from three main sources provided by the authors: published sources (comprising peer-reviewed articles, books, and theses), unpublished data, and herbarium data. We compiled a data set composed of 2,095 species, from 89,270 holo/hemiepiphyte records, in the Atlantic Forest of Brazil, Argentina, Paraguay, and Uruguay, recorded from 1824 to early 2018. Most of the records were from qualitative data (occurrence only, 88%), well distributed throughout the Atlantic Forest. For quantitative records, the most common sampling method was individual trees (71%), followed by plot sampling (19%), and transect sampling (10%). Angiosperms (81%) were the most frequently registered group, and Bromeliaceae and Orchidaceae were the families with the greatest number of records (27,272 and 21,945, respectively). Ferns and Lycophytes presented fewer records than Angiosperms, and Polypodiaceae were the most recorded family, and more concentrated in the Southern and Southeastern regions. Data on non-vascular plants and lichens were scarce, with a few disjunct records concentrated in the Northeastern region of the Atlantic Forest. For all non-vascular plant records, Lejeuneaceae, a family of liverworts, was the most recorded family. We hope that our effort to organize scattered epiphyte data help advance the knowledge of epiphyte ecology, as well as our understanding of macroecological and biogeographical patterns in the Atlantic Forest. No copyright restrictions are associated with the data set. Please cite this Ecology Data Paper if the data are used in publication and teaching events. © 2019 The Authors. Ecology © 2019 The Ecological Society of Americ

Otimização da utilização de marcadores moleculares microssatélites e sua aplicação em estudos com plantas daninhas

Apesar de existirem marcadores moleculares mais específicos, os marcadores microssatélites apresentam grande potencialidade de utilização na área de plantas daninhas devido à sua crescente disponibilização em outras espécies e à qualidade das informações proporcionadas. O uso convencional dos marcadores moleculares microssatélites demanda grande quantidade de trabalho e recursos financeiros. O objetivo deste trabalho foi descrever a técnica da cauda fluorescente como forma de otimização da utilização de marcadores moleculares microssatélites, utilizando como exemplo um estudo de identificação de híbridos entre arroz-vermelho e cultivado. Foram utilizadas como modelo plantas de arroz cultivado, arroz-vermelho e o híbrido originado do cruzamento artificial dessas plantas. A técnica da cauda fluorescente consiste na síntese do iniciador forward com a sequência desejada e a adição da sequência de um iniciador universal, que corresponde à chamada cauda. A detecção da amplificação é realizada em equipamento de eletroforese capilar automatizada, através da utilização de um iniciador universal sintetizado com fluoróforo. O sistema desenvolvido foi eficiente na identificação da hibridização entre arroz cultivado e vermelho e apresenta viabilidade de utilização, por exemplo, em estudos de fluxo gênico da resistência a herbicidas e de caracteres relacionados à adaptação diferencial entre essas plantas. A técnica da cauda fluorescente possibilitou o uso de diversos marcadores moleculares a partir de um único marcador fluorescente e viabilizou a realização das análises em multiplex. O aumento da disponibilidade e do conhecimento de técnicas moleculares pode proporcionar melhor elucidação em vários estudos relacionados a espécies de plantas daninhas que possuem pouca disponibilidade de marcadores moleculares específicos

Genetic Structure And Phenotypic Variation In Wild Populations Of The Medicinal Tetraploid Species Bromelia Antiacantha (bromeliaceae)

Premise of the study: The patterns of genetic structure in plant populations are mainly related to the species life history and breeding system, and knowledge of these patterns is necessary for the management, use, and conservation of biological diversity. Polyploidy is considered an important mode of evolution in plants, but few studies have evaluated genetic structure of polyploid populations. We studied the patterns of genetic structure and morphological variation of Bromelia antiacantha (Bromeliaceae) populations, a polyploid terrestrial species.Methods: Microsatellite markers and morphological analyses were used to explore patterns of genetic and morphological diversity in wild populations of B. antiacantha.Key results: The results of our simple-sequence repeat analyses supported that B. antiacantha is a polyploid species. The inbreeding coefficients were high and significant in all populations (F IS = 0.431), indicating homozygote excess. Bromelia antiacantha showed high levels of genetic differentiation among populations (F ST = 0.224) and therefore was highly structured. High morphological variation was observed in fruit phenotypic traits in the populations studied.Conclusions: The levels of genetic diversity and the pattern of the population ' s structure may be related to the low recruitment of seeds, clonal reproduction, and the population ' s colonization history. The genetic and morphological variability displayed in this study are important issues in planning the conservation and exploitation of this resource in a sustainable way. © 2011 Botanical Society of America.98915111519Barbará, T., Lexer, C., Martinelli, G., Mayo, S., Fay, M.F., Heuertz, M., Within-population spatial genetic structure in four naturally fragmented species of a neotropical inselberg radiation, Alcantarea imperialis, A. geniculata, A. glaziouana and A. regina (Bromeliaceae) (2008) Heredity, 101, pp. 285-296Barbará, T., Martinelli, G., Fay, M.F., Mayo, J., Lexer, C., Population differentiation and species cohesion in two closely related plants adapted to neotropical high-altitude 'inselbergs,'Alcantarea imperialis and Alcantarea geniculata (Bromeliaceae) (2007) Molecular Ecology, 16, pp. 1981-1992Barbará, T., Martinelli, G., Palma-Silva, C., Fay, M.F., Mayo, S., Lexer, C., Genetic relationships and variation in reproductive strategies in four closely related bromeliads adapted to neotropical 'inselbergs': Alcantarea glaziouana, A. regina, A. geniculata and A. imperialis (Bromeliaceae) (2009) Annals of Botany, 103, pp. 65-77Barbará, T., Palma-Silva, C., Paggi, G.M., Bered, F., Fay, M.F., Lexer, C., Cross-species transfer of nuclear microsatellites markers: Potential and limitations (2007) Molecular Ecology, 16, pp. 3759-3767Borba, E.L., Funch, R.R., Ribeiro, P.L., Smidt, E.C., Silva-Pereira, V., Demography, and genetic and morphological variability of the endangered Sophronitis sincorana (Orchidaceae) in the Chapada Diamantina, Brazil (2007) Plant Systematics and Evolution, 267, pp. 129-146Brown, G.K., Gilmartin, A.J., Chromosome numbers in Bromeliaceae (1989) American Journal of Botany, 76, pp. 657-665Canela, M.B.F., Sazima, M., The pollination of Bromelia antiacantha (Bromeliaceae) in southeastern Brazil: Ornithophilous versus melittophilous features (2005) Plant Biology, 7, pp. 411-416Carnaval, A.C., Moritz, C., Historical climate modelling predicts patterns of current biodiversity in the Brazilian Atlantic forest (2008) Journal of Biogeography, 35, pp. 1187-1201Cascante-Marín, A., Oostermeijer, J.G.B., Wolf, J.H.D., den Nijs, J.C.M., Reproductive biology of the epiphytic bromeliad Werauhia gladioliflora in a premontane tropical forest (2005) Plant Biology, 7, pp. 203-209Cavallari, M.M., Forzza, R.C., Veasey, E.A., Zucchi, M.I., Oliveira, G.C.X., Genetic variation in three endangered species of Encholirium (Bromeliaceae) from Cadeia do Epinhaço, Brazil, detected using RAPD markers (2006) Biodiversity and Conservation, 15, pp. 4357-4373Ceita, G.O., Assis, J.G.A., Guedes, M.L.S., Oliveira, A.N.P.C., Cytogenetics of Brazilian species of Bromeliaceae (2008) Botanical Journal of Linnean Society, 158, pp. 189-193Chapman, H.M., Parh, D., Oraguzie, N., Genetic structure and colonizing success of a clonal, weedy species, Pilosella officinarum (Asteraceae) (2000) Heredity, 84, pp. 401-409Crawford, D.J., Enzyme electrophoresis and plant systematics (1989) Isozymes in plant biology. Dioscorides Press, , In D. E. Soltis and P. S. Soltis [eds.], Portland, Oregon, USADoyle, J.J., Doyle, J.L., Isolation of plant DNA from fresh tissue (1990) Focus (San Francisco, Calif.), 12, pp. 13-15Duarte, A.S., da Silva, C.V., Puchalski, A., Mantovani, M., Silva, J.Z., Reis, M.S., Estrutura demográfica e produçã o de frutos de Bromelia antiacantha Bertol (2007) Revista Brasileira de Plantas Medicinais, 9, pp. 106-112Estoup, A., Jarne, P., Cornuet, J.M., Homoplasy and mutation model at microsatellite model and their consequences for population genetics analysis (2002) Molecular Ecology, 11, pp. 1591-1604Evanno, G., Regnaut, S., Goudet, J., Detecting the numbers of clusters of individuals using the software Structure: A simulation study (2005) Molecular Ecology, 14, pp. 2611-2620Gitaí, J.S.F., (2006) Contribuiçã o de Características Citogenéticas e MolecularesàSistemática de Bromeliaceae, , Ph.D. dissertation, Universidade Federal de Pernambuco, Recife, BrazilGitaí, J., Horres, R., Benko-Iseppon, A.M., Chromosomal features and evolutions of Bromeliaceae (2005) Plant Systematics and Evolution, 253, pp. 65-80Givnish, T.J., Barfus, M.H.J., van Ee, B., Riina, R., Schulte, K., Horres, R., Gonsiska, P.A., Phylogeny, adaptive radiation, and historical biogeography in Bromeliaceae: Insights from an eightlocus plastid phylogeny (2011) American Journal of Botany, 98, pp. 872-895Gliddon, C., Belhassen, E., Gouyon, P.H., Genetic neighborhoods in plants with diverse systems of mating and different patterns of growth (1987) Heredity, 59, pp. 29-32González-Astorga, J., Cruz-Angón, A., Flores-Palacios, A., Vovides, A.P., Diversity and genetic structure of the Mexican endemic epiphyte Tillandsia achyrostachys E. Morr. ex Baker var. achyrostachys (Bromeliaceae) (2004) Annals of Botany, 94, pp. 545-551Gower, J.C., A general coefficient of similarity and some of its properties (1971) Biometrics, 27, pp. 857-874Hartl, D.L., Clark, A.G., (1997) Principles of populations genetics, 3rd ed, , Sinauer, Sunderland, Massachusetts, USAHmeljevski, K.V., Reis, A., Montagna, T., Reis, M.S., Genetic diversity, genetic drift and mixed mating system in small subpopulations of Dyckia ibiramensis, a rare endemic bromeliad from Southern Brazil (2011) Conservation Genetics, 12, pp. 761-769Hokanson, K., Hancock, J., Levels of allozymic diversity in diploid and tetraploid Vaccinium sect. Cyanococcus (blueberries) (1998) Canadian Journal of Plant Science, 78, pp. 327-332Jorge, L.I.F., Ferro, V.O., Reconhecimento da espécie Bromelia antiacantha Bertol. Características botânicas e fitoquímicas (1993) Revista de Farmacia y Bioquímica, 29, pp. 69-72López-Pujol, J., Bosch, M., Simon, J., Blanche, C., Allozyme diversity in the tetraploid endemic Thymus loscosii (Lamiaceae) (2004) Annals of Botany, 93, pp. 323-332Lorscheitter, M.L., Contribution to the Holocene history of Atlantic rain forest in the Rio Grande do Sul State, southern Brazil (2003) Revista del Museo Argentino de Ciencia Naturales, 5, pp. 261-271Murawski, A.D., Hamrick, J.L., Local genetic and clonal structure in the tropical terrestrial bromeliad, Aechmea magdalenae (1990) American Journal of Botany, 77, pp. 1201-1208Nei, M., Analysis of gene diversity in subdivided populations (1973) Proceedings of the National Academy of Sciences, USA, 70, pp. 3321-3323Nei, M., F-statistics and analysis of gene diversity in subdivided populations (1977) Annals of Human Genetics, 41, pp. 225-233Norero, N., Malleville, J., Huarte, M., Feingold, S., Cost efficient potato (Solanum tuberosum L.) cultivar identification by microsatellite amplification (2002) Potato Research, 45, pp. 131-138Nybom, H., Comparison on different nuclear DNA markers for estimating intraspecific genetic diversity in plants (2004) Molecular Ecology, 13, pp. 1143-1155Ota, T., (1993) DISPAN: Genetic distance and phylogenetic analysis, , http://homes.bio.psu.edu/people/Faculty/Nei/, Computer program and documentation distributed by the author, website: [accessed 10 December 2009]Otto, S.P., Whitton, J., Polyploid incidence and evolution (2000) Annual Review of Genetics, 34, pp. 401-437Paggi, G.M., Palma-Silva, C., Cidade, F.W., Sousa, A.C.B., Souza, A.P., Wendt, T., Lexer, C., Bered, F., Isolation and characterization of microsatellite loci in Pitcairnia albiflos (Bromeliaceae), an endemic bromeliad from Atlantic Rainforest, and cross-amplification in other species (2008) Molecular Ecology Research, 8, pp. 980-982Palma-Silva, C., Cavallari, M.M., Barbará, T., Lexer, C., Gimenes, A., Bered, F., Bodanese-Zanettini, M.H., A set of polymorphic microsatellite loci for Vreisea gigantea and Alcantarea imperialis (Bromeliaceae) and cross-amplification in other bromeliads species (2007) Molecular Ecology Notes, 7, pp. 654-657Palma-Silva, C., Lexer, C., Paggi, G.M., Barbará, T., Bered, F., Bodanese-Zanettini, M.H., Range-wide patterns of nuclear and chloroplast DNA diversity in Vriesea gigantea (Bromeliaceae), a neotropical forest species (2009) Heredity, 103, pp. 503-512Palma-Silva, C., Santos, D.G., Kaltchuk-Santos, E., Bodanese-Zanettini, M.H., Chromosome numbers, meiotic behavior, and pollen viability of species Vriesea and Aechmea genera (Bromeliaceae) native to Rio Grande do Sul, Brazil (2004) American Journal of Botany, 91, pp. 804-807Pillar, V.D., (2008) Multiv, software for multivariate exploratory analysis, randomization testing and bootstrap resampling (for Macintosh and Windows OS), , http://ecoqua.ecologia.ufrgs.br, Computer program and documentation distributed by the author, website: [accessed 5 December 2009]Pritchard, J.K., Stephens, M., Donnelly, P., Inference of population structure using multilocus genotype data (2000) Genetics, 155, pp. 945-959Prober, S.M., Spindler, L.H., Brown, A.H.D., Conservation of the grassy white box woodlands: Effects of remnant population size on genetic diversity in the allotetraploid herb Microseris lanceolata (1998) Conservation Biology, 12, pp. 1279-1290Ramsey, J., Schemske, D.W., Pathways, mechanisms, and rates of polyploid formation in flowering plants (1998) Annual Review of Ecology and Systematics, 29, pp. 467-501Rohlf, F.J., (2000) NTSYS-pc numerical taxonomy and multivariate analysis system, version 2.11a, , Exeter Software, Setauket, New York, USASantos, D.S., Puchalski, A., Gomes, G.S., Mantovani, M., Silva, J.Z., Reis, M.S., Variaçã o no período de germinaçã o de sementes em uma populaçã o natural de Bromelia antiacantha Bertol (2004) Revista Brasileira de Plantas Medicinais, 6, pp. 35-41Sarthou, C., Samadi, S., Boisseler-Dubayle, M.C., Genetic structure of the saxicole Pitcairnia geyskesii (Bromeliaceae) on inselbergs in French Guiana (2001) American Journal of Botany, 88, pp. 861-868Sgorbati, S., Labra, M., Gruni, E., Barcaccia, G., Galasso, G., Boni, U., Mucciarelli, M., A survey of genetic diversity and reproductive biology of Puya raimondii (Bromeliaceae), the endangered queen of the Andes (2004) Plant Biology, 6, pp. 222-230Smith, L.B., Downs, R.J., (1979) Bromelioideae (Bromeliaceae), 3. , Flora Neotropica. Monograph 14, Hafner Press, New York, New York, USASoltis, D.E., Gilmartin, J., Rieseberg, L., Gardner, S., Genetic variation in the epiphytes Tillandsia ionantha and T. recurvata (Bromeliaceae) (1987) American Journal of Botany, 74, pp. 531-537Soltis, P.S., Soltis, D.E., The role of genetic and genomic attributes in the success of polyploids. National Academy of Sciences colloquium: Variation and evolution in plants and microorganisms: Toward a new synthesis 50 years after Stebbins (2000) Proceedings of the National Academy of Sciences, 97, pp. 7051-7705. , June 20, 2000, Irvine, California, USA., USAStebbins, G.L., Polyploidy in plants: Unresolved problems and prospects (1980) Polyploidy, pp. 495-520. , In W. H. Lewis [ed.], Plenum Press, New York, New York, USAStebbins Jr., G.L., Types of polyploids: Their classification and significance (1947) Advances in Genetics, 1, pp. 403-429Stiles, F.G., Geographical aspects of bird flower coevolution with particular reference to Central America (1981) Annals of the Missouri Botanical Garden, 68, pp. 323-351Thomé, M.T., Zamudio, K.R., Giovanelli, J.G.R., Haddad, C.F.B., Baldissera, F.A., Alexandrino, J., Phylogeography of endemic toads and post-Pliocene persistence of the Brazilian Atlantic Forest (2010) Molecular Phylogenetics and Evolution, 55, pp. 1018-1031Thrall, P.H., Young, A., AUTOTET: A program for analysis of autotetraploid genotypic data (2000) Journal of Heredity, 91, pp. 348-34