Wool sheep and purple snails - Long‐term continuity of animal exploitation in ancient Meninx (Jerba/Tunisia)

Archaeological research at the ancient city of Meninx in Jerba, Tunisia, carried out by the Institut National du Patrimoine Tunisie and Ludwig Maximilian University of Munich (LMU) produced more than 10,000 faunal specimens and shed light on subsistence activities spanning from the fourth century BCE until the seventh century CE. Despite its highly diverse fauna totalling at least 69 species of mammals, birds, reptiles, fish and molluscs, domestic livestock formed the mainstay of the economy at Meninx. Throughout site occupation and compared with contemporaneous sites in coastal Tunisia and Libya, sheep were of prime importance at Meninx. Diachronic demographic profiling illustrates an emphasis on the production of wool for making textiles. Together with the ubiquitous presence of crushed banded dye‐murex (Hexaplex trunculus) shells implying exploitation of purple dyes, we assume that both activities were integrated into a single chaîne opératoire for making purple‐dyed fabrics that were traded across the Mediterranean from Punic until Late Roman times. Zooarchaeological findings also suggest that during the Byzantine Period, this major economic activity came to a standstill, with people returning to more self‐sufficient subsistence strategies. An intersite comparison furthermore revealed that high proportions of ovicaprines are a typical feature of Punic–Roman sites in Jerba. But even at the height of Roman power in the region, autochthonous husbandry traditions continued to exist on the island, as illustrated by the fauna from Henchir Bourgou

Dramatic Shape Sensitivity of Directional Emission Patterns from Similarly Deformed Cylindrical Polymer Lasers

Recent experiments on similarly shaped polymer micro-cavity lasers show a dramatic difference in the far-field emission patterns. We show for different deformations of the ellipse, quadrupole and hexadecapole that the large differences in the far-field emission patterns is explained by the differing ray dynamics corresponding to each shape. Analyzing the differences in the appropriate phase space for ray motion, it is shown that the differing geometries of the unstable manifolds of periodic orbits are the decisive factors in determining the far-field pattern. Surprisingly, we find that strongly chaotic ray dynamics is compatible with highly directional emission in the far-field.Comment: 14 pages, 16 figures (eps), RevTeX 4, submitted to JOSA

A facile method for the preparation of bifunctional Mn:ZnS/ZnS/Fe3O4 magnetic and fluorescent nanocrystals

Bifunctional magnetic and fluorescent core/shell/shell Mn:ZnS/ZnS/Fe3O4 nanocrystals were synthesized in a basic aqueous solution using 3-mercaptopropionic acid (MPA) as a capping ligand. The structural and optical properties of the heterostructures were characterized by X-ray diffraction (XRD), dynamic light scattering (DLS), transmission electron microscopy (TEM), UV–vis spectroscopy and photoluminescence (PL) spectroscopy. The PL spectra of Mn:ZnS/ZnS/Fe3O4 quantum dots (QDs) showed marked visible emission around 584 nm related to the 4T1 → 6A1 Mn2+ transition. The PL quantum yield (QY) and the remnant magnetization can be regulated by varying the thickness of the magnetic shell. The results showed that an increase in the thickness of the Fe3O4 magnetite layer around the Mn:ZnS/ZnS core reduced the PL QY but improved the magnetic properties of the composites. Nevertheless, a good compromise was achieved in order to maintain the dual modality of the nanocrystals, which may be promising candidates for various biological applications

Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function

Porcine Reproductive and Respiratory Syndrome (PRRS) is a panzootic infectious disease of pigs, causing major economic losses to the world-wide pig industry. PRRS manifests differently in pigs of all ages but primarily causes late-term abortions and stillbirths in sows and respiratory disease in piglets. The causative agent of the disease is the positive-strand RNA PRRS virus (PRRSV). PRRSV has a narrow host cell tropism, limited to cells of the monocyte/macrophage lineage. CD163 has been described as a fusion receptor for PRRSV, whereby the scavenger receptor cysteine-rich domain 5 (SRCR5) region was shown to be an interaction site for the virus in vitro. CD163 is expressed at high levels on the surface of macrophages, particularly in the respiratory system. Here we describe the application of CRISPR/Cas9 to pig zygotes, resulting in the generation of pigs with a deletion of Exon 7 of the CD163 gene, encoding SRCR5. Deletion of SRCR5 showed no adverse effects in pigs maintained under standard husbandry conditions with normal growth rates and complete blood counts observed. Pulmonary alveolar macrophages (PAMs) and peripheral blood monocytes (PBMCs) were isolated from the animals and assessed in vitro. Both PAMs and macrophages obtained from PBMCs by CSF1 stimulation (PMMs) show the characteristic differentiation and cell surface marker expression of macrophages of the respective origin. Expression and correct folding of the SRCR5 deletion CD163 on the surface of macrophages and biological activity of the protein as hemoglobin-haptoglobin scavenger was confirmed. Challenge of both PAMs and PMMs with PRRSV genotype 1, subtypes 1, 2, and 3 and PMMs with PRRSV genotype 2 showed complete resistance to viral infections assessed by replication. Confocal microscopy revealed the absence of replication structures in the SRCR5 CD163 deletion macrophages, indicating an inhibition of infection prior to gene expression, i.e. at entry/fusion or unpacking stages

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

Experimental investigation of hot-work tool steels performances under the creep-fatigue regime

In the present research, an innovative testing method, specifically developed to characterize the tool steels under creep-fatigue conditions, was carried out on a TQ1 (X36CrMoV5-2) hot-work tool steel in cooperation with Constellium R&D center. The experimental campaign consisted of different testing conditions, and most of the specimens were nitrided to account for the specific surface state of the tools. Tests were performed on a 100 kN MTS fatigue machine equipped with a heating furnace. A creep-fatigue loading type was applied to the specimens, i.e., a cyclic load with a dwell time, in order to properly reproduce the conditions acting on extrusion tools and dies. Under a constant temperature of 520 °C, the effects of four different load levels and two different values of dwell times were evaluated. In addition, selected test conditions were replicated with specimens not nitrided with the aim to evaluate and quantify the influence of the superficial treatment. Final results were presented in terms of fatigue diagram of the TQ1 steel and compared to the performances of the H11 tool steel tested in a previous research by the same authors

Assessment on the creep-fatigue performances of tool steels

Extrusion dies are typically produced by hot work steels with a great diffusion of AISI H-11 (1.2343) and AISI H-13 (1.2344) steels. Nevertheless different steels for hot working application are available on the market and several new steel grades are proposed every year for this type of application. A main concern for extruder and die maker that are interested into testing new steel grades, is how to evaluate the performances of the new material. If the material is directly applied for manufacturing a die, even if the die is an identical duplication of a previously tested, no reliable data are usually obtained as consequence of process variance. On the other side, standard testing (like tensile testing), even when performed at several high temperatures, offer only a partial view of the mechanical behavior of an extrusion die. Indeed dies are not affected by static loading being fatigue and creep the most damaging mechanisms of an extrusion die properly designed. In the present paper an innovative testing method, (previously proposed by the authors in [1] for investigating separately fatigue, creep and creep-ftigue interaction), was carried out on a TQ1 (X36CrMoV5-2) hot-work tool steel in cooperation with Constellium R&D center. A creep-fatigue loading type was applied to the specimens, i.e. a cyclic load with a dwell-time, in order to properly reproduce the conditions acting on extrusion dies. The specimens were tested at four different loading conditions (from 26 to 45 kN), wirth two different dwell times (160-300 s) and also with different surface conditions (tempered/tempered plus nitriding). Tests were performed on a 100kN MTS fatigue machine equipped with a heating furnace and the temperature was kept constant at 520°C. Final results were presented in terms of fatigue diagram curve of the TQ1 steel and compared to the performances of the H11 tool steel tested in a previous research by the same authors

Stabilization of β-Ca2SiO4

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The βγ transformation of Ca2SiO4

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