Post-mission Viking data anaysis

Three Mars data analysis projects from the Viking Mars program were identified initially, and three more came into being as the work proceeded. All together, these six pertained to: (1) the veritical distribution of scattering particles in the Martian atmosphere at various locations in various seasons, (2) the physical parameters that define photometric properties of the Martian surface and atmosphere, (3) patterns of dust-cloud and global dust-storm development, (4) a direct comparison of near-simultaneous Viking and ground-based observations, (5) the annual formation and dissipation of polar frost caps, and (6) evidence concerning possible present-day volcanism or venting. A list of publications pertaining to the appropriate projects is included

Of gene expression and cell division time: a mathematical framework for advanced differential gene expression and data analysis

Estimating fold changes of average mRNA and protein molecule counts per cell is the most common way to perform differential expression analysis. However, these gene expression data may be affected by cell division, an often-neglected phenomenon. Here, we develop a quantitative framework that links population-based mRNA and protein measurements to rates of gene expression in single cells undergoing cell division. The equations we derive are easy-to-use and widely robust against biological variability. They integrate multiple "omics" data into a coherent, quantitative description of single-cell gene expression and improve analysis when comparing systems or states with different cell division times. We explore these ideas in the context of resting versus activated B cells. Analyzing differences in protein synthesis rates enables to account for differences in cell division times. We demonstrate that this improves the resolution and hit rate of differential gene expression analysis when compared to analyzing population protein abundances alone

Sediment-moss interactions on a temperate glacier: Falljökull, Iceland

Full text of this article can be found at: http://www.igsoc.org/annals/ Copyright IGS. DOI: 10.3189/172756408784700734We present the results of preliminary investigations of globular moss growth on the surface of Falljökull, a temperate outlet glacier of the Vatnajökull ice cap, southern Iceland. Supraglacial debris has provided a basis for moss colonization, and several large (>500 m2) patches of moss growth (Racomitrium spp.) are observed on the surface of the glacier. Each area of moss-colonized supraglacial debris shows a downslope increase in sphericity and moss cushion size and a decrease in percentage surface coverage of moss-colonized and bare clasts. It is suggested that moss growth on supraglacial debris allows preferential downslope movement of clasts through an associated increase in both overall mass and sphericity. Thermal insulation by moss cushions protects the underlying ice surface from melt, and the resulting ice pedestals assist in downslope sliding and toppling of moss cushions. The morphology and life cycle of supraglacial globular mosses is therefore not only closely linked to the presence and distribution of supraglacial debris, but also appears to assist in limited down-glacier transport of this debris. This research highlights both the dynamic nature of the interaction of mosses with supraglacial sedimentary systems and the need for a detailed consideration of their role within the wider glacial ecosystem.Peer reviewe

Bycatch of lined seahorses (Hippocampus erectus) in a Gulf of Mexico shrimp trawl fishery

Bycatch studies have largely ignored population level effects on fish species of little commercial interest. Here we analyze bycatch of the lined seahorse (Hippocampus erectus) in the bait-shrimp trawl fishery in Hernando Beach, Florida, providing the first fisheries data for this species. Based on catch per unit of effort (CPUE), size, sex, and reproductive status of trawled H. erectus, 1) approximately 72,000 seahorses were caught annually by this fleet, from a population of unknown size, 2) trawling affected population cohorts differentially because of temporal and spatial variation in CPUE and population size, and 3) a greater proportion of females than males was removed in trawling. Our findings suggest that trawling may affect seahorse populations through direct mortality, social disruption, and habitat damage. However, the lack of specific abundance or catchability estimates for H. erectus means that the precise impact of trawling on this fish remains uncertain. This paper focuses attention on the need for research and monitoring of small fishes that are caught incidentally in nonselective gear

Spatially resolved spectra of 3C galaxy nuclei

We present and discuss visible-wavelength long-slit spectra of four low redshift 3C galaxies obtained with the STIS instrument on the Hubble Space Telescope. The slit was aligned with near-nuclear jet-like structure seen in HST images of the galaxies, to give unprecedented spatial resolution of the galaxy inner regions. In 3C 135 and 3C 171, the spectra reveal clumpy emission line structures that indicate outward motions of a few hundred km s$^{-1}$ within a centrally illuminated and ionised biconical region. There may also be some low-ionisation high-velocity material associated with 3C 135. In 3C 264 and 3C 78, the jets have blue featureless spectra consistent with their proposed synchrotron origin. There is weak associated line emission in the innermost part of the jets with mild outflow velocity. These jets are bright and highly collimated only within a circumnuclear region of lower galaxy luminosity, which is not dusty. We discuss the origins of these central regions and their connection with relativistic jets.Comment: 15 pages incl Tables, 12 diagrams, To appear in A

Digging for Dark Matter: Spectral Analysis and Discovery Potential of Paleo-Detectors

Paleo-detectors are a recently proposed method for the direct detection of Dark Matter (DM). In such detectors, one would search for the persistent damage features left by DM--nucleus interactions in ancient minerals. Initial sensitivity projections have shown that paleo-detectors could probe much of the remaining Weakly Interacting Massive Particle (WIMP) parameter space. In this paper, we improve upon the cut-and-count approach previously used to estimate the sensitivity by performing a full spectral analysis of the background- and DM-induced signal spectra. We consider two scenarios for the systematic errors on the background spectra: i) systematic errors on the normalization only, and ii) systematic errors on the shape of the backgrounds. We find that the projected sensitivity is rather robust to imperfect knowledge of the backgrounds. Finally, we study how well the parameters of the true WIMP model could be reconstructed in the hypothetical case of a WIMP discovery.Comment: 14 pages, 5 figures, code available at https://github.com/tedwards2412/paleo_detectors/ . v2: Added additional analysis theory details, matches version published in PR

Elastic Wave Propagation and Scattering in Austenitic Steel

Ultrasonic nondestructive testing of austenitic steel welds is very difficult, because fundamental wave propagation and scattering effects in such complicated anisotropic environments are only hardly understood [1, 2]. Therefore, a step-by-step evaluation of elastic wave propagation in transversely isotropic media has been initiated. Under the assumption of transverse isotropy the numerical EFIT code (Elastodynamic Finite Integration Technique) [3] - [7] was extended to anisotropic homogeneous media. It allows 3D computation of quasi pressure and quasi shear as well as surface waves in transverse isotropic media. Results for finite aperture transducer radiation and crack scattering in a single crystal austenitic weld are presented; measurements of amplitude dynamics, A-Scans and C-Scans confirm the EFIT simulations [8]

Kinetics of the Multiferroic Switching in MnWO4_4

The time dependence of switching multiferroic domains in MnWO$_4$ has been studied by time-resolved polarized neutron diffraction. Inverting an external electric field inverts the chiral magnetic component within rise times ranging between a few and some tens of milliseconds in perfect agreement with macroscopic techniques. There is no evidence for any faster process in the inversion of the chiral magnetic structure. The time dependence is well described by a temperature-dependent rise time suggesting a well-defined process of domain reversion. As expected, the rise times decrease when heating towards the upper boundary of the ferroelectric phase. However, switching also becomes faster upon cooling towards the lower boundary, which is associated with a first-order phase transition

Large-Scale Outflows in Edge-on Seyfert Galaxies. III. Kiloparsec-Scale Soft X-ray Emission

We present ROSAT PSPC and HRI images of eight galaxies selected from a distance-limited sample of 22 edge-on Seyfert galaxies. Kiloparsec-scale soft X-ray nebulae extend along the galaxy minor axes in three galaxies (NGC 2992, NGC 4388 and NGC 5506). The extended X-ray emission has 0.2-2.4 keV X-ray luminosities of $0.4-3.5 \times 10^{40} erg s^{-1}$. The X-ray nebulae are roughly co-spatial with the large-scale radio emission, suggesting that both are produced by large-scale galactic outflows. Assuming pressure balance between the radio and X-ray plasmas, the X-ray filling factor is \gapprox 10^4 times larger than the radio plasma filling factor, suggesting that large-scale outflows in Seyfert galaxies are predominantly winds of thermal X-ray emitting gas. We favor an interpretation in which large-scale outflows originate as AGN-driven jets that entrain and heat gas on kpc scales as they make their way out of the galaxy. AGN- and starburst-driven winds are also possible explanations in cases where the winds are oriented along the rotation axis of the galaxy disk.Comment: 24 pages, 7 ps figures, AASTEX 4.0, accepted for ApJ April 1, 199

A dual paper-based nucleic acid extraction method from blood in under ten minutes for point-of-care diagnostics.

Nucleic acid extraction (NAE) plays a crucial role for diagnostic testing procedures. For decades, dried blood spots (DBS) have been used for serology, drug monitoring, and molecular studies. However, extracting nucleic acids from DBS remains a significant challenge, especially when attempting to implement these applications to the point-of-care (POC). To address this issue, we have developed a paper-based NAE method using cellulose filter papers (DBSFP) that operates without the need for electricity (at room temperature). Our method allows for NAE in less than 7 min, and it involves grade 3 filter paper pre-treated with 8% (v/v) igepal surfactant, 1 min washing step with 1× PBS, and 5 min incubation at room temperature in 1× TE buffer. The performance of the methodology was assessed with loop-mediated isothermal amplification (LAMP), targeting the human reference gene beta-actin and the kelch 13 gene from P. falciparum. The developed method was evaluated against FTA cards and magnetic bead-based purification, using time-to-positive (min) for comparative analysis. Furthermore, we optimised our approach to take advantage of the dual functionality of the paper-based extraction, allowing for elution (eluted disk) as well as direct placement of the disk in the LAMP reaction (in situ disk). This flexibility extends to eukaryotic cells, bacterial cells, and viral particles. We successfully validated the method for RNA/DNA detection and demonstrated its compatibility with whole blood stored in anticoagulants. Additionally, we studied the compatibility of DBSFP with colorimetric and lateral flow detection, showcasing its potential for POC applications. Across various tested matrices, targets, and experimental conditions, our results were comparable to those obtained using gold standard methods, highlighting the versatility of our methodology. In summary, this manuscript presents a cost-effective solution for NAE from DBS, enabling molecular testing in virtually any POC setting. When combined with LAMP, our approach provides sample-to-result detection in under 35 minutes