Spatial knowledge management and participatory governance: rethinking the trajectories of urban, socio-economic and environmental change and the politics of 'sustainability' in southern cities

This paper presents the analytical framework developed iteratively by the research team of the Chance2Sustain (C2S) research project between 2010 and July 2014, in order to answer the main research question which was posed at the outset of the research, namely : how can spatial knowledge management (SKM) and participatory governance contribute to sustainable urban development ? To answer this question, the C2S project was designed to undertake comparative empirical research in 10 cities in four fast-growing countries of the South to understand the role of SKM and participatory processes in facing the challenges in a number of strategic domains of urban development ; those of economic growth, social inequality and vulnerability, and environmental governance. In each city, there were researchers from both the North and South working together in the five domains of economic growth through megaprojects ; social mobilisation and social exclusion in sub-standard settlements ; environmental governance with the focus on water-related issues ; spatial knowledge management; and fiscal decentralisation and participatory city budgeting

Generation of precision-cut slice cultures of human placenta.

We present a protocol to generate an advanced ex vivo model of human placenta. We use a vibrating tissue slicer to obtain precision-cut slices representative of the entire thickness of human placenta. This approach delivers standardized cultures with a preserved microstructure and cellular composition comparable to the native tissue. We applied this system to study SARS-CoV-2 infection at the maternal-fetal interface. Moreover, this system can be used to investigate the basic functions of the human placenta in health and disease. For complete details on the use and execution of this protocol, please refer to Fahmi et al. (2021)

Кон'юнктурний аналіз розвитку ринку рекреаційних послуг АР Крим

Метою дослідження є кон’юнктурний аналіз розвитку ринку рекреаційних послуг АР Крим та порівняльна оцінка функціонування конкурентоспроможних рекреаційних районів

A Novel Cellular Model to Study Angiotensin II AT2 Receptor Function in Breast Cancer Cells

Recent studies have highlighted the AT1 receptor as a potential therapeutic target in breast cancer, while the role of the AT2 subtype in this disease has remained largely neglected. The present study describes the generation and characterization of a new cellular model of human invasive breast cancer cells (D3H2LN-AT2) stably expressing high levels of Flag-tagged human AT2 receptor (Flag-hAT2). These cells exhibit high-affinity binding sites for AngII, and total binding can be displaced by the AT2-selective antagonist PD123319 but not by the AT1-selective antagonist losartan. Of interest, high levels of expression of luciferase and green fluorescent protein make these cells suitable for bioluminescence and fluorescence studies in vitro and in vivo. We provide here a novel tool to investigate the AT2 receptor functions in breast cancer cells, independently of AT1 receptor activation