Localization of Na pumps in the tracheal epithelium of the dog.

Binding of [3H]ouabain by the dog's tracheal epithelium shows a nonspecific component depending linearly on ouabain concentration, and a specific saturable component with a Km of 10(-7) M. Control experiments showed that the tracer taken up was not trapped within the extracellular space nor bound to tissue collagen. Inhibition of the saturable uptake by high K, metabolic inhibition, low Na, and low temperature indicated that binding was to Na/K ATPase. One-sided exposures of tissue sheets to tracer showed that the submucosal side took up 10 X as much tracer as the luminal. Autoradiography localized tracer uptake under all conditions to the cells' basolateral membranes

Cientifizando McLuhan: predicados do acoplamento homem-máquina, a hipótese do tríplice isomorfismo e suas consequências estéticas

In Laws of Media, written with his son Eric, Marshall McLuhan aimed to assign scientific status to his ideas, presenting his Tetrad model and offering several examples of its application. Mcluhanism places great emphasis on media ecology specific perceptual bias; authors such as Maurice Merleau-Ponty, Humberto Maturana, Francisco Varela, David Chalmers and Andy Clark allow us to bridge it to the research on consciousness and brain carried through the last decades. If media creates an experience related to its epistemic context, it is possible to approach the forms of human-machine coupling – especially in digital culture –, by examining the hypothesis of a triplex isomorphism between <brain><apparatus><experience> that would turn mcluhanism into hard science. This hypothesis’ aspects are discussed, alongside further problems related to contemporary artwork creation. Na obra Leis da Mídia, escrita com seu filho Eric, Marshall McLuhan pretendeu atribuir um status científico às suas ideias, apresentando seu modelo em Tétrade e oferecendo vários exemplos de sua aplicação. O mcluhanismo enfatiza fortemente o viés perceptual específico da ecologia da mídia; autores como Maurice Merleau-Ponty, Humberto Maturana, Francisco Varela, David Chalmers e Andy Clark nos permitem associá-lo à pesquisa sobre consciência e cérebro das últimas décadas. Se a mídia cria uma experiência relacionada ao seu contexto epistêmico, é possível analisar formas de acoplamento homem-máquina – especialmente na cultura digital – ao examinar a hipótese de um tríplice isomorfismo entre <cérebro><aparato><experiência>, que transformaria o mcluhanismo em ciência dura. Aspectos desta hipótese são discutidos, juntamente a outros problemas relacionados à criação de obras de arte contemporânea.&nbsp

Mispositioned Neurokinin-1 Receptor-Expressing Neurons Underlie Heat Hyperalgesia in Disabled-1 Mutant Mice.

Reelin (Reln) and Disabled-1 (Dab1) participate in the Reln-signaling pathway and when either is deleted, mutant mice have the same spinally mediated behavioral abnormalities, increased sensitivity to noxious heat and a profound loss in mechanical sensitivity. Both Reln and Dab1 are highly expressed in dorsal horn areas that receive and convey nociceptive information, Laminae I-II, lateral Lamina V, and the lateral spinal nucleus (LSN). Lamina I contains both projection neurons and interneurons that express Neurokinin-1 receptors (NK1Rs) and they transmit information about noxious heat both within the dorsal horn and to the brain. Here, we ask whether the increased heat nociception in Reln and dab1 mutants is due to incorrectly positioned dorsal horn neurons that express NK1Rs. We found more NK1R-expressing neurons in Reln-/- and dab1-/- Laminae I-II than in their respective wild-type mice, and some NK1R neurons co-expressed Dab1 and the transcription factor Lmx1b, confirming their excitatory phenotype. Importantly, heat stimulation in dab1-/- mice induced Fos in incorrectly positioned NK1R neurons in Laminae I-II. Next, we asked whether these ectopically placed and noxious-heat responsive NK1R neurons participated in pain behavior. Ablation of the superficial NK1Rs with an intrathecal injection of a substance P analog conjugated to the toxin saporin (SSP-SAP) eliminated the thermal hypersensitivity of dab1-/- mice, without altering their mechanical insensitivity. These results suggest that ectopically positioned NK1R-expressing neurons underlie the heat hyperalgesia of Reelin-signaling pathway mutants, but do not contribute to their profound mechanical insensitivity

Trabalho de arte/Evento curatorial

Neste texto são discutidas algumas questões relacionadas à práticacuratorial, tendo como referência a relação entre o gesto de construção da exposição enquanto evento-obra e o contato com obra de arte como dispositivo singular: na medida em que as fronteiras entre exposição e obra se tornam cada vez menos nítidas, como organizar a ativação da exposição enquanto ativação da obra e vice-versa? Em que medida a construção da exposição, ao colocar em movimento interesses diversos, intensifica ou não o contato do visitante com a obra, quando este é cada vez mais capturado pela intensidade do evento-exposição? Do mesmo modo, serão discutidos os limites entre os papéis do curador e do artista, em proximidade também com o crítico, o historiador e outros agentes do circuito da arte contemporânea