Salmonella and tomatoes

Outbreak information linking fresh tomato fruit to illnesses is reviewed in this chapter. While tomato fruit appear to support substantial proliferation of certain serovars of Salmonella enterica, detection of this pathogen in tomato plants prior to harvest is rare, and reports of Salmonella existence in tomato fruit still attached to field-grown plants are virtually non-existent. The bacterium is sensitive to UV and can be outcompeted by the native phytomicrobiota, which may explain its absence in field-grown crops. However, the persistence of certain serovars in fields and ponds of certain production areas is noted. Together with evidence of bacteria becoming internalized in tomato fruit during crop development likely through natural apertures, the presence of S. enterica in and around production fields suggests that an unusual weather event could lead to Salmonella contamination of fruit prior to harvest. The bacterium appears physiologically adaptive toward proliferation in tomato fruit. Once inside tomatoes, Salmonella is capable of sensing the availability of nutrients and physiological state of the fruit and differentially regulates specific genes. However, because Salmonella is an efficient nutrient scavenger, removal of multiple metabolic and regulatory genes was required to reduce its fitness within the fruit. Plants do not appear to recognize human enterics as pathogens, and their defenses treat them as endophytes

Study of the Three Component System Diethyl Ether-Water-Magnesium Bromide at 25 C. (Abstract)

Varying amounts of the three components were brought together in a tube and allowed to come to equilibrium at 25° C. and at atmospheric pressure. The composition of each of the condensed phases, solid and liquid, was determined by analysis and a triangular three component diagram of the phases in equilibrium was made. From the areas, lines and points of the phase diagram, the following information was obtained. Two independent pairs of partly miscible liquids were found: one consisting of a water and an ether layer with a small amount of magnesium bromide dissolved in the ether layer, the other pair consisting essentially of two ether layers, with considerable magnesium bromide in one of the layers. Two solids were found that can exist in equilibrium with liquid phases at 25° C., these being magnesium bromide hexahydrate and magnesium bromide di-ether ate. No evidence was found for the existence of a mixed solvate or the basic magnesium bromide di-etherate as claimed by Menschutkin

Using earthworms as bioindicators of quality in no-till systems.

No Brasil, o plantio direto é o sistema de manejo do solo mais praticado. Dentre os diversos benefícios, o plantio direto promove as funções dos organismos, especialmente as minhocas, sobre a fertilidade do solo. Portanto, o presente estudo teve como objetivo avaliar as populações de minhocas, visando uma classificação da densidade e diversidade de minhocas em áreas sob plantio direto. As coletadas foram realizadas em fevereiro/março de 2010 e 2011, num total de 34 e 25 propriedades respectivamente em seis municípios da Bacia do Paraná 3, parte da bacia do lago de Itaipu. As minhocas foram amostradas no final da estação chuvosa, utilizando uma adaptação do Método para Biologia e Fertilidade em Solos Tropicais (TSBF), consistindo na coleta de 5 monolitos de 20 x 20 por 20 cm de profundidade, espaçados por mínimo 20 m um do outro, em um transecto linear. Foi realizada triagem manual no campo e as minhocas foram estocadas em sacos plásticos contendo solução de formaldeído 5%. As minhocas foram contadas e identificadas em nível de família, gênero e espécies. A simplificação da metodologia de amostragem objetivou possibilitar que os próprios agricultores avaliem as populações de minhocas em suas propriedades. A abundância de minhocas variou de 5 a 600 ind m-2 e 5 a 1150 ind m-2, respectivamente nas épocas de coletas em 2010 e 2011, enquanto o número de espécies variou de uma a seis em 2010 e de uma a sete em 2011 nas áreas sob plantio direto. Baseado neste estudo e em resultados de outros estudos anteriores, uma classificação para abundância e diversidade de minhocas em áreas sob plantio direto é proposta aqui, para Latossolos Vermelhos e regiões de clima quente do Paraná (Cfa Koeppen) : pobre >25 ind m-2 and 1 sp.; moderado ? 25 to 6 sp.Disponível online