Rooting and carbohydrate availability in Vitis 140 Ruggeri stem cuttings

Rooting in Vitis 140 Ruggeri rootstock appears to be correlated to the availability of soluble carbohydrates in the cuttings. This relationship was verified using stem cuttings collected from December to April by comparing those propagated after cold storage (C) with those collected directly in the field (F). The basal end of some of the cuttings of both groups (C and F) were dipped into deionized water before propagation (CW and FW). The mobilization of soluble carbohydrates paralleled with the pattern of the rooting process. In the cold stored material, rooting took place earlier than in the field material. The mobilization of soluble carbohydrates occurred at two stages: during the storage period at 2 °C (50% if compared with the cuttings collected in the field) and during the first 20 d of rooting. On day 20, the carbohydrate content was reduced by 80%. Rooting and carbohydrate availability appear to be associated

Egg quality from nera atriana, a local poultry breed of the Abruzzo region (Italy), and isa brown hens reared under free range conditions

The selection and diffusion in the poultry sector of hybrid breeds able to produce higher amount of meat and eggs, led over time to the erosion of genetic resources. One of the strategies that can be applied in order to stem such phenomenon, concerns the valorization of the animal products, meat or eggs, obtained from indigenous poultry breeds. Therefore, the aim of this study is to characterize the qualitative aspects of eggs obtained from the Nera Atriana hen, an Italian indigenous laying hen characteristic of the Abruzzo region, making a direct comparison with a commercial hybrid reared in the same environment and applying the same feeding protocol. The trial was conducted for a period of 5 months (from March to July 2019), in which 6 egg samplings were performed (one at the beginning and additional 5 on a monthly basis); each sampling involved the collection of 15 eggs per group of animals for a total of 90 eggs per genotype. Eggs were specifically subjected to evaluations of the physical parameters, including the yolk color, and analyses aimed at determining the content of total lipids, cholesterol, and β-carotene. In addition to this, the fatty acids profile and the electrophoretic pattern of the globular proteins of the yolk were characterized. Eggs from hybrid hens were characterized by higher total weight with significantly higher values specifically associated to albumen and yolk weight and to the albumen percentage. In the eggs obtained from the local hen, higher relative percentages were instead found in the quantity of shell and yolk; furthermore, in the same samples was observed a higher yolk redness, a cholesterol concentration tending to be lower although not significant (p = 0.0521), and a greater amount of saturated fatty acids which could justify a lower susceptibility of the food to oxidative processes following transformation with heat treatments. With regard to the fatty acid composition, differences were specifically observed for palmitic (C16:0) and palmitoleic (C16:1 cis-9) acids that showed higher relative percentages in eggs from local breed, and for the oleic acid (C18:1 cis-9) which was more represented in eggs from commercial hens. In conclusion, some interesting nutritional features, in a preliminary way, have been highlighted that could lead the consumer to prefer these “niche” products over those obtained from commercial hybrids

Polymorphisms of seed storage proteins in Olea europaea L. cultivars

The use of Olea europaea globulins as genetic markers for the identification of the cultivars was perfomed in the laboratory of Genetics of Department of Agriculture, Food and Environment, University of Pisa. The major component of Olea europaea L. seed storage proteins is represented by globulins. These fractions were characterised by electrophoresis and compared with the proteins extracted from protein bodies. The biochemical analysis of the olive seed globulins was carried out in sixteen different cultivars coming from several geographical areas of Italy. The electrophoretic patterns in polyacrylamide gels electrophoresis in denaturing conditions (SDS-PAGE) evidenced both qualitative and quantitative differences. It was possible to identify all the cultivars by their electrophoretic spectra. Number and position of the electrophoretic bands allowed the construction of a similarity matrix and of a dendrogram that allowed the separation into groups, according to their phylogenetic relationships. The several clusters seem to be related with agronomic traits such as fruit size or oil production; no relationships were found with the geographical cultivation area

Esca symptoms appearance in Vitis vinifera L.: influence of climate, pedo-climatic conditions and rootstock/cultivar combination

This study investigated the appearance of esca symptoms in relation to environmental factors and the rootstock/cultivar combination in an experimental setting between 2004 and 2009. Among the common genotypes showing susceptibility to the esca disease, four cultivars were considered: ‘Cabernet Sauvignon’, ‘Sangiovese’, ‘Trebbiano Toscano’ and ‘Chardonnay’. These cultivars were studied own-rooted and in combination with two rootstocks: Kober 5BB and 1103 Paulsen. The difference in susceptibility of cultivars to esca appeared negatively related to the graft. No clear relation was found between esca appearance and environmental factors. Moreover, an unexpected discordance between esca incidence percentage and mortality rate was observed.

Reliability analysis of wireless sensor network for smart farming applications

Wireless Sensor Networks are subjected to some design constraints (e.g., processing capability, storage memory, energy consumption, fixed deployment, etc.) and to outdoor harsh conditions that deeply affect the network reliability. The aim of this work is to provide a deeper understanding about the way redundancy and node deployment affect the network reliability. In more detail, the paper analyzes the design and implementation of a wireless sensor network for low-power and low-cost applications and calculates its reliability considering the real environmental conditions and the real arrangement of the nodes deployed in the field. The reliability of the system has been evaluated by looking for both hardware failures and communication errors. A reliability prediction based on different handbooks has been carried out to estimate the failure rate of the nodes self-designed and self-developed to be used under harsh environments. Then, using the Fault Tree Analysis the real deployment of the nodes is taken into account considering the Wi-Fi coverage area and the possible communication link between nearby nodes. The findings show how different node arrangements provide significantly different reliability. The positioning is therefore essential in order to obtain maximum performance from a Wireless sensor network