Performance of Beef Heifers of Various Genetic Groups, Supplemented or Not, in Coastcross Pastures

The objective of this study was to determine whether the performance of beef heifers of different genetic groups was affected by breed x nutritional environment interactions. Sixty four weaned heifers, 16 per genetic group: ½ Angus + ½ Nellore (AN), ½ Canchim + ½ Nellore (CN), ½ Simmental + ½ Nellore (SN) and pure Nellore (NE), were used with or without 3.0 kg of concentrate.animal-1 .day-1 in a fertilized coastcross pasture under rotational grazing system. There were effects of genetic group and supplementation (P\u3c 0.05) on the weight and age at first estrus, but there was no interaction between them. In a rotational grazing system with 4000 kg of available dry matter per hectare with 13% of crude protein, the crossbred AN, supplemented or not, was more precocious (111 days) than Nellore heifers, showing the first estrus at 356 days of age and 324 kg of live weight

Electrochemical removal of CuII in the presence of humic acid

The removal of CuII (10 mg L-1) complexed by commercial humic acid (100 mg L-1) was studied at different current densities (30-80 mA cm-2) using a filter-press cell, Ti/Ru0.3Ti0.7O2 anode and stainless steel cathode. The electrolyses were performed with and without membrane separating the cell compartments [(homogeneous anionic (Nafion R117), heterogeneous cationic (Ionac MC-3470) and heterogeneous anionic (Ionac MA-3475)]. The influence of the membrane on the rate of CuII removal was bigger for cationic membranes. Current efficiencies up to ca. 55% (Ionac MC-3470) for CuII removal at 30 mA cm-2 were obtained. By combining oxidation and reduction, 100% CuII removal was achieved at current efficiencies of 75%. The main path for the removal is the electrostatic interaction with the anode followed by diffusion through the membrane and deposition at the cathode. Additionally, the oxidative degradation of humic acid contributes to the rate of CuII removal.A remoção de CuII (10 mg L-1) complexado por ácido húmico comercial (100 mg L-1) foi estudada a diferentes densidades de corrente (30-80 mA cm-2) usando uma célula filtro-prensa, ânodo de Ti/Ru0.3Ti0.7O2 e cátodo de aço inoxidável. As eletrólises foram feitas com e sem membrana separando os compartimentos da célula [aniônica homogênea (Nafion R117), catiônica heterogênea (Ionac MC-3470) e aniônica heterogênea (Ionac MA-3475)]. A influência da membrana na velocidade de remoção de CuII foi maior com as catiônicas. Foi obtida eficiência de corrente de ca. 55% (Ionac MC-3470) para remoção de CuII a 30 mA cm-2. Combinando oxidação e redução, foi obtida remoção de 100% de CuII com eficiência de corrente de 75%. A etapa principal para a remoção é interação eletrostática com o ânodo, seguida pela difusão através da membrana e deposição sobre o cátodo. Adicionalmente, a degradação oxidativa de ácido húmico contribui com a velocidade de remoção de CuII.651658Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Glyceraldehyde-3-phosphate dehydrogenase of Paracoccidioides brasiliensis is a cell surface protein involved in fungal adhesion to extracellular matrix proteins and interaction with cells

The pathogenic fungus Paracoccidioides brasiliensis causes paracoccidioidomycosis, a pulmonary mycosis acquired by inhalation of fungal airborne propagules, which may disseminate to several organs and tissues, leading to a severe form of the disease. Adhesion to and invasion of host cells are essential steps involved in the infection and dissemination of pathogens. Furthermore, pathogens use their surface molecules to bind to host extracellular matrix components to establish infection. Here, we report the characterization of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of P. brasiliensis as an adhesin, which can be related to fungus adhesion and invasion. the P. brasiliensis GAPDH was overexpressed in Escherichia coli, and polyclonal antibody against this protein was obtained. By immunoelectron microscopy and Western blot analysis, GAPDH was detected in the cytoplasm and the cell wall of the yeast phase of P. brasiliensis. the recombinant GAPDH was found to bind to fibronectin, laminin, and type I collagen in ligand far-Western blot assays. of special note, the treatment of P. brasiliensis yeast cells with anti-GAPDH polyclonal antibody and the incubation of pneumocytes with the recombinant protein promoted inhibition of adherence and internalization of P. brasiliensis to those in vitro-cultured cells. These observations indicate that the cell wall-associated form of the GAPDH in P. brasiliensis could be involved in mediating binding of fungal cells to fibronectin, type I collagen, and laminin, thus contributing to the adhesion of the microorganism to host tissues and to the dissemination of infection.Univ Fed Goias, Inst Ciencias Biol, Mol Biol Lab, BR-74001970 Goiania, Go, BrazilUniv Brasilia, BR-70910900 Brasilia, DF, BrazilUniv Estadual Julio Mesquita Filho, Araraquara, SP, BrazilUniversidade Federal de São Paulo, São Paulo, BrazilUniversidade Federal de São Paulo, São Paulo, BrazilWeb of Scienc

Do Diabetic Patients with Acute Coronary Syndromes Have a Higher Threshold for Ischemic Pain?

Background: Data from over 4 decades have reported a higher incidence of silent infarction among patients with diabetes mellitus (DM), but recent publications have shown conflicting results regarding the correlation between DM and presence of pain in patients with acute coronary syndromes (ACS). Objective: Our primary objective was to analyze the association between DM and precordial pain at hospital arrival. Secondary analyses evaluated the association between hyperglycemia and precordial pain at presentation, and the subgroup of patients presenting within 6 hours of symptom onset. Methods: We analyzed a prospectively designed registry of 3,544 patients with ACS admitted to a Coronary Care Unit of a tertiary hospital. We developed multivariable models to adjust for potential confounders. Results: Patients with precordial pain were less likely to have DM (30.3%) than those without pain (34.0%; unadjusted p = 0.029), but this difference was not significant after multivariable adjustment, for the global population (p = 0.84), and for subset of patients that presented within 6 hours from symptom onset (p = 0.51). In contrast, precordial pain was more likely among patients with hyperglycemia (41.2% vs 37.0% without hyperglycemia, p = 0.035) in the overall population and also among those who presented within 6 hours (41.6% vs. 32.3%, p = 0.001). Adjusted models showed an independent association between hyperglycemia and pain at presentation, especially among patients who presented within 6 hours (OR = 1.41, p = 0.008). Conclusion: In this non-selected ACS population, there was no correlation between DM and hospital presentation without precordial pain. Moreover, hyperglycemia correlated significantly with pain at presentation, especially in the population that arrived within 6 hours from symptom onset

Results of the MWPC gas gain uniformity tests performed at CERN

We present the results of the gas gain uniformity tests performed as part of the quality control of the multiwire proportional chambers produced at CERN for the LHCb Muon system, along with a description of the last hardware and software upgrades. The test provides a relative gas gain measurement over the whole chamber sensitive area. It is based on the analysis of the spectrum obtained when the chamber is exposed to a $^{241}$Am radioactive source. Since the measurement is normalized to the peak of a precise pulse generator, the gain uniformity can also be evaluated among different gas gaps and different chambers

Main assumptions for energy pathways

© The Author(s) 2019. The aim of this chapter is to make the scenario calculations fully transparent and comprehensible to the scientific community. It provides the scenario narratives for the reference case (5.0 °C) as well as for the 2.0 °C and 1.5 °C on a global and regional basis. Cost projections for all fossil fuels and renewable energy technologies until 2050 are provided. Explanations are given for all relevant base year data for the modelling and the main input parameters such as GDP, population, renewable energy potentials and technology parameters

Exercise training in the aerobic/anaerobic metabolic transition prevents glucose intolerance in alloxan-treated rats

<p>Abstract</p> <p>Background</p> <p>Ninety percent of cases of diabetes are of the slowly evolving non-insulin-dependent type, or Type 2 diabetes. Lack of exercise is regarded as one of the main causes of this disorder. In this study we analyzed the effects of physical exercise on glucose homeostasis in adult rats with type 2 diabetes induced by a neonatal injection of alloxan.</p> <p>Methods</p> <p>Female Wistar rats aged 6 days were injected with either 250 mg/kg of body weight of alloxan or citrate buffer 0.01 M (controls). After weaning, half of the animals in each group were subjected to physical training adjusted to meet the aerobic-anaerobic metabolic transition by swimming 1 h/day for 5 days a week with weight overloads. The necessary overload used was set and periodically readjusted for each rat through effort tests based on the maximal lactate steady state procedure. When aged 28, 60, 90, and 120 days, the rats underwent glucose tolerance tests (GTT) and their peripheral insulin sensitivity was evaluated using the HOMA index.</p> <p>Results</p> <p>The area under the serum glucose curve obtained through GTT was always higher in alloxan-treated animals than in controls. A decrease in this area was observed in trained alloxan-treated rats at 90 and 120 days old compared with non-trained animals. At 90 days old the trained controls showed lower HOMA indices than the non-trained controls.</p> <p>Conclusion</p> <p>Neonatal administration of alloxan induced a persistent glucose intolerance in all injected rats, which was successfully counteracted by physical training in the aerobic/anaerobic metabolic transition.</p