Efeito da solarização e biofumigação, durante o outono, na incidência de murcha-bacteriana e produtividade da batata.

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Obtenção de geléia de açaí branco (Euterpe oleracea, Mart Var. Branco).

O açaizeiro (Euterpe oleracea, Mart) é uma palmeira exuberante da flora tropical, que a partir de seus frutos, obtém-se uma bebida substancial e nutritiva. Destacando-se a variedade açaí branco, pouco aproveitado na região Norte, mas que necessita ter a propagação da espécie para gerar aumento em produção e comercialização, pois agora o açaí desperta não só o interesse nacional como internacional. O presente trabalho teve por objetivo a produção de geléia artesanal a partir da bebida açaí branco com adição de componentes naturais, como uma alternativa de conservação agregando assim valor à bebida e proporcionando melhoria de renda para a população rural. Foram realizados ensaios laboratoriais para a obtenção de uma geléia à base de albedo de laranja (fonte de pectina), suco de limão (acidulante) e açúcar (sacarose de cana de açúcar), produto A e outra geléia utilizando pectina comercial (de alto grau de metoxilação), suco de limão e açúcar, produto B. Realizaram-se determinações das características físico-químicas e microbiológicas para a matéria-prima e para o produto final, e utilizou-se análise sensorial nos produtos A e B para verificar a sua aceitação. Os resultados das análises microbiológicas para o produto final atestaram sua produção dentro de condições higiênico-sanitárias satisfatórias. A análise sensorial evidenciou que o produto A obteve maior aceitação que o produto B. A geléia com albedo apresentou 76,16% de aceitação, pH de 4,01 (baixa acidez), teor em proteínas (1,05%) e de alto valor energético (281,80 Kcal/100g). O processamento de geléia de açaí branco usando albedo de laranja, suco de limão e açúcar é viável podendo vir a ser um futuro próximo aproveitado pelas agroindústrias como uma das maneiras de conservar o açaí branco

Detection of mcr-1 Gene in undefined Vibrio species isolated from clams

The increase of antimicrobial resistant strains is leading to an emerging threat to public health. Pathogenic Vibrio are responsible for human and animal illness. The Enterobacteriaceae family includes microorganisms that affect humans, causing several infections. One of the main causes of human infection is related to the ingestion of undercooked seafood. Due to their filterfeeding habit, marine invertebrates, such as clams, are known to be a natural reservoir of specific microbial communities. In the present study, Vibrionaceae and coliforms microorganisms were isolated from clams. A microbial susceptibility test was performed using the disk diffusion method. From 43 presumptive Vibrio spp. and 17 coliforms, three Vibrio spp. with MICs to colistin > 512 mg L−1 were found. From the 23 antimicrobial resistance genes investigated, only the three isolates that showed phenotypic resistance to colistin contained the mcr‐1 gene. Genotypic analysis for virulence genes in EB07V indicated chiA gene presence. The results from the plasmid cure and transformation showed that the resistance is chromosomally mediated. Biochemical analysis and MLSA, on the basis of four protein‐coding gene sequences (recA, rpoB, groEL and dnaJ), grouped the isolates into the genus Vibrio but distinguished them as different from any known Vibrio spp.info:eu-repo/semantics/publishedVersio

A Rare Case of Mediastinal Mass: Thymoma and Thymic Tumor

Thymomas and thymic carcinomas are rare mediastinal neoplasms arising from thymic epithelial cells, and the presence of synchronous or metachronous primary thymic neoplasms in a single patient is an extremely rare event. Thymoma patients appear to have an inherent predisposition toward developing additional neoplasms. This additionally presents a diagnostic challenge, revealing the importance of multidisciplinary expertise to the management of these patients. This is a case report of a patient with a thymoma and thymic carcinoma, submitted to surgical resection and postoperative radiotherapyinfo:eu-repo/semantics/publishedVersio

Colorimetric Assessment of BCR-ABL1 Transcripts in Clinical Samples Via Gold Nanoprobes

Gold nanoparticles functionalized with thiolated oligonucleotides (Au-nanoprobes) have been used in a range of applications for the detection of bioanalytes of interest, from ions to proteins and DNA targets. These detection strategies are based on the unique optical properties of gold nanoparticles, in particular, the intense color that is subject to modulation by modification of the medium dieletric. Au-nanoprobes have been applied for the detection and characterization of specific DNA sequences of interest, namely pathogens and disease biomarkers. Nevertheless, despite its relevance, only a few reports exist on the detection of RNA targets. Among these strategies, the colorimetric detection of DNA has been proven to work for several different targets in controlled samples but demonstration in real clinical bioanalysis has been elusive. Here, we used a colorimetric method based on Au-nanoprobes for the direct detection of the e14a2 BCR-ABL fusion transcript in myeloid leukemia patient samples without the need for retro-transcription. Au-nanoprobes directly assessed total RNA from 38 clinical samples, and results were validated against reverse transcription-nested polymerase chain reaction (RT-nested PCR) and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The colorimetric Au-nanoprobe assay is a simple yet reliable strategy to scrutinize myeloid leukemia patients at diagnosis and evaluate progression, with obvious advantages in terms of time and cost, particularly in low- to medium-income countries where molecular screening is not routinely feasible. Graphical abstract Gold nanoprobe for colorimetric detection of BCR-ABL1 fusion transcripts originating from the Philadelphia chromosome

Solarização do solo e biofumigação no cultivo protegido de tomate.

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General Framework for phase synchronization through localized sets

We present an approach which enables to identify phase synchronization in coupled chaotic oscillators without having to explicitly measure the phase. We show that if one defines a typical event in one oscillator and then observes another one whenever this event occurs, these observations give rise to a localized set. Our result provides a general and easy way to identify PS, which can also be used to oscillators that possess multiple time scales. We illustrate our approach in networks of chemically coupled neurons. We show that clusters of phase synchronous neurons may emerge before the onset of phase synchronization in the whole network, producing a suitable environment for information exchanging. Furthermore, we show the relation between the localized sets and the amount of information that coupled chaotic oscillator can exchange

Avaliação de ferramentas na furação de laminados compósitos

Drilling of polymeric matrix composite structuresThe development of cutting tools becomes a challenge to the industry of composite materials to obtain high quality holes, minimizing damage. This work aims to examine the influence of various parameters such as feed rate and tool geometry on thrust forces developed during drilling operations and on the extent of damage. In this work, a comparative study of tool geometry with the aim of reducing the associated damage during drilling process is presented. In the end a good combination of tool geometry and drilling parameters based on experimental work will be presented.O desenvolvimento de ferramentas de corte torna-se um desafio à indústria de materiais compósitos, na obtenção de furos de elevada qualidade, minimizando o dano. Este trabalho visa analisar a influência de vários parâmetros, tais como o avanço e a geometria de ferramenta, nas forças desenvolvidas durante a furação e na extensão do dano. Neste artigo é apresentado um estudo comparativo de geometrias de broca com o objectivo de reduzir o dano associado à furação, que permitirá determinar qual a melhor selecção de parâmetros de furação, atendendo às diferentes geometrias de ferramenta