Division of Natural Products Chemistry

この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されまし

Department of Natural Products Chemistry

I.天然薬物成分の科学的研究東南アジア(インドネシア, ネパール, ベトナム, タイ, ミャンマー等)の薬用植物II.和漢薬成分の医薬化学1.羅布麻, プロポリスから単離した生理活性成分の合成2.肝臓病や骨粗鬆症に有効な天然薬物成分の開発研究3.マトリックスメタロプロテアーゼ産生阻害を有する天然薬物成分の研究4.紅豆杉の活性成分の研究III.漢方製剤の品質評価1.法通関丸, 桃核承気湯, 当帰飲子など2.基源植物, 修治生薬のLC-MSによる評価IV.和漢薬成分の生物有機化学的研究ベルベリン, ベトナム人参, CAPE類縁体, キナ酸誘導体, カリクシン類, 花楓成分などV.天然薬物調査ベトナム, ミャンマー, ブラジルなどこの論文は国立情報学研究所の学術雑誌公開支援事業により電子化されまし

Department of Products Chemistry

I. 天然、薬物成分の科学的研究東南アジア（インドネシア，ネパール，ベトナム， タイ， ミャンマ一等）の薬用植物II. 和漢薬成分の医薬化学1. 羅布麻，プロポリスから単離した生理活性成分の合成2. 肝臓病や骨組懸症に有効な天然薬物成分の開発研究3. マトリックスメタロプロテアーゼ産生阻害を有する天然薬物成分の研究4. 紅豆杉の活性成分の研究III. 漢方製剤の品質評価法1. 通関丸，桃核承気湯，当帰飲子など2. 基源植物， 修治生薬のLC-MSによる評価IV. 和漢薬成分の生物有機化学的研究ベルベリン，ベトナム人参， CAPE類縁体，キナ酸誘導体，カリクシン類，花椴成分などv. 天然薬物調査ベトナム， ミャンマー，ブラジルなどこの論文は国立情報学研究所の学術雑誌公開支援事業により電子化されまし

Neuroprotective effects of the cultivated Chondrus crispus in a C. elegans model of Parkinson's disease

Parkinson's disease (PD) is the second most common neurodegenerative disorder in the elderly people, currently with no cure. Its mechanisms are not well understood, thus studies targeting cause-directed therapy or prevention are needed. This study uses the transgenic Caenorhabditis elegans PD model. We demonstrated that dietary supplementation of the worms with an extract from the cultivated red seaweed Chondrus crispus decreased the accumulation of \u3b1-synulein and protected the worms from the neuronal toxin-, 6-OHDA, induced dopaminergic neurodegeneration. These effects were associated with a corrected slowness of movement. We also showed that the enhancement of oxidative stress tolerance and an up-regulation of the stress response genes, sod-3 and skn-1, may have served as the molecular mechanism for the C. crispus-extract-mediated protection against PD pathology. Altogether, apart from its potential as a functional food, the tested red seaweed, C. crispus, might find promising pharmaceutical applications for the development of potential novel anti-neurodegenerative drugs for humans.Peer reviewed: YesNRC publication: Ye

Lipids isolated from the cultivated red alga Chondrus crispus inhibit nitric oxide production

A MeOH extract of cultivated Chondrus crispus showed dose-dependent nitric oxide (NO) inhibition of lipopolysaccharide-induced NO production in macrophage RAW264.7 cells. NO inhibition-guided fractionation of the extract led to identification of eicosapentaenoic acid (EPA, 1), arachidonic acid (AA, 2), lutein (3), and eight galactolipids as active components. Based on spectral analysis, the isolated galactolipids were identified as (2S)-1,2-bis-O-eicosapentaenoyl-3-O-\u3b2-d-galactopyranosylglycerol (4), (2S)-1-O-eicosapentaenoyl-2-O-arachidonoyl-3-O-\u3b2-d-galactopyranosylglycerol (5), (2S)-1-O-(6Z,9Z,12Z,15Z-octadecatetranoyl)-2-O-palmitoyl-3-O-\u3b2-d-galactopyranosylglycerol (6), (2S)-1-O-eicosapentaenoyl-2-O-palmitoyl-3-O-\u3b2-d-galactopyranosylglycerol (7), (2S)-1,2-bis-O-arachidonoyl-3-O-\u3b2-d-galactopyranosylglycerol (8), (2S)-1-O-arachidonoyl-2-O-palmitoyl-3-O-\u3b2-d-galactopyranosylglycerol (9), (2S)-1-O-eicosapentaenoyl-2-O-palmitoyl-3-O-(\u3b2-d-galactopyranosyl-6-1\u3b1-d-galactopyranosyl)-glycerol (10), and (2S)-1-O-arachidonoyl-2-O-palmitoyl-3-O-(\u3b2-d-galactopyranosyl-6-1\u3b1-d-galactopyranosyl)-glycerol (11). All the isolated compounds showed significant NO inhibitory activity. This is the first report of the isolation and identification of individual galactolipids from C. crispus. Moreover, (2S)-1,2-bis-O-arachidonoyl -3-O-\u3b2-d-galactopyranosylglycerol (8) is a novel compound.Peer reviewed: YesNRC publication: Ye

Polar lipids from the marine macroalga Palmaria palmata inhibit lipopolysaccharide-induced nitric oxide production in RAW264.7 macrophage cells

The EtOAc soluble fraction of a MeOH/CHCl3 extract of Palmaria palmata showed strong nitric oxide (NO) inhibitory activity against lipopolysaccharide (LPS)-induced NO production in murine RAW264.7 cells. NO inhibition-guided isolation led to identification of three new polar lipids including a sulfoquinovosyl diacylglycerol (SQDG) (2S)-1-O-eicosapentaenoyl-2-O- myristoyl-3-O-(6-sulfo-\u3b1-d-quinovopyranosyl)-glycerol (1) and two phosphatidylglycerols, 1-O-eicosapentaenoyl-2-O-trans-3-hexadecenoyl-3-phospho- (1\u2032-glycerol)-glycerol (3) and 1-O-eicosapentaenoyl-2-O-palmitoyl-3- phospho-(1\u2032-glycerol)-glycerol (4) from the EtOAc fraction. Seven known lipids were also isolated including a SQDG (2), a phospholipid (5) and five galactolipids (6-10). Structures of the isolated lipids were elucidated by spectral analyses. The isolated SQDGs, phosphatidylglycerols and phospholipid possessed strong and dose-dependent NO inhibitory activity compared to N G-methyl-l-arginine acetate salt (l-NMMA), a well-known NO inhibitor used as a positive control. Further study suggested that these polar lipids suppressed NO production through down-regulation of inducible nitric oxide synthase (iNOS).Peer reviewed: YesNRC publication: Ye