Effects of dynamic cell culture conditions on 3D spatial reogarnization of MSCS/HUVECS spheroids in the context of bone tissue

We aim to observe the development and reorganization of MSCs and HUVECs in Pullulan/Dextran hydrogels supplied with nHAp under dynamic culture conditions in a perfusion bioreactor.Hydrogel poreux sous perfusion : modélisation et optimisation d'un modèle in vitro de reconstruction de défaut osseu

Bone Spheroid Development Under Flow Conditions with Mesenchymal Stem Cells and Human Umbilical Vein Endothelial Cells in a 3D Porous Hydrogel Supplemented with Hydroxyapatite

International audienceUnderstanding the niche interactions between blood and bone through the in vitro co-culture of osteo-competent cells and endothelial cells is a key factor in unraveling therapeutic potentials in bone regeneration. This can be additionally supported by employing numerical simulation techniques to assess local physical factors, such as oxygen concentration, and mechanical stimuli, such as shear stress, that can mediate cellular communication. In this study, we developed a Mesenchymal Stem Cell line (MSC) and a Human Umbilical Vein Endothelial Cell line (HUVEC), which were co-cultured under flow conditions in a three-dimensional, porous, natural pullulan/dextran scaffold that was supplemented with hydroxyapatite crystals that allowed for the spontaneous formation of spheroids. After 2 weeks, their viability was higher under the dynamic conditions (>94%) than the static conditions (<75%), with dead cells central in the spheroids. Mineralization and collagen IV production increased under the dynamic conditions, correlating with osteogenesis and vasculogenesis. The endothelial cells clustered at the spheroidal core by day 7. Proliferation doubled in the dynamic conditions, especially at the scaffold peripheries. Lattice Boltzmann simulations showed negligible wall shear stress in the hydrogel pores but highlighted highly oxygenated zones coinciding with cell proliferation. A strong oxygen gradient likely influenced endothelial migration and cell distribution. Hypoxia was minimal, explaining high viability and spheroid maturation in the dynamic conditions

Effects of dynamic cell culture conditions on 3D spatial reogarnization of MSCS/HUVECS spheroids in the context of bone tissue

International audienceWe aim to observe the development and reorganization of MSCs and HUVECs in Pullulan/Dextran hydrogels supplied with nHAp under dynamic culture conditions in a perfusion bioreactor

Effects of dynamic cell culture conditions on 3D spatial reogarnization of MSCS/HUVECS spheroids in the context of bone tissue

International audienceWe aim to observe the development and reorganization of MSCs and HUVECs in Pullulan/Dextran hydrogels supplied with nHAp under dynamic culture conditions in a perfusion bioreactor

Co-culture of HUVECs and hMSCs in a Perfusion Bioreactor Ensures the Development of Viable Spheroids

International audienc

Co-culture of HUVECs and hMSCs in a Perfusion Bioreactor Ensures the Development of Viable Spheroids

International audienc

Perfused porous hydrogel scaffold: modeling hydrodynamics and species transport

International audiencePresent approach well suited to predict both (i) fluid flow in the macroporosity of the scaffold stack, (ii) the oxygen state experienced by the cells within the scaffolds Next: Co-culture of human umbilical vein endothelial cells and human mesenchymal stem cells Optical coherence tomography (OCT) to get a better spatial resolution and capture the microporosity of the porous hydrogel scaffolds Context LBM scheme and boundary conditions Custom-made perfusion bioreactor Hydrodynamics and oxygen transport

Perfused porous hydrogel scaffold: modeling hydrodynamics and species transport

Present approach well suited to predict both (i) fluid flow in the macroporosity of the scaffold stack, (ii) the oxygen state experienced by the cells within the scaffolds Next: Co-culture of human umbilical vein endothelial cells and human mesenchymal stem cells Optical coherence tomography (OCT) to get a better spatial resolution and capture the microporosity of the porous hydrogel scaffolds Context LBM scheme and boundary conditions Custom-made perfusion bioreactor Hydrodynamics and oxygen transport.Hydrogel poreux sous perfusion : modélisation et optimisation d'un modèle in vitro de reconstruction de défaut osseuxLABORATORY FOR SYSTEMS AND ENGINEERING OF PARIS SACLAYIdex Paris-Sacla

How hydrodynamics and species transport influence the 3D microenvironment of cells seeded in hydrogel scaffolds

International audienc